Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,432,9850AG21.9% 60.8 / 12.1 32intergenic (‑43/+27)folC/accDbifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
Reads supporting (aligned to +/- strand):  ref base A (5/20);  new base G (4/3);  total (9/23)
Fisher's exact test for biased strand distribution p-value = 7.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCG  >  NC_000913_3_hsa_tpiA/2432837‑2433128
                                                                                                                                                    |                                                                                                                                               
tCACGCGCTCAAGGCAGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCagag                                                                                                                                                >  4:227410/1‑150 (MQ=255)
             gCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGAGGCTTGAGGAGTGCGTTTGATACACATGGTATCCGCTGCTTCGCTCCGGTAAGAATAGCAAAAGGGCAGAGCCAGTGgccctgc                                                                                                                                   <  1:61815/150‑1 (MQ=255)
                gagaTCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCaga                                                                                                                                                 <  2:349008/133‑1 (MQ=255)
                gagaTCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCaga                                                                                                                                                 >  1:349008/1‑133 (MQ=255)
                   aTCGATAGTTTTACTGTGCAGGTTTTCCAGACAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttat                                                                                                                             <  2:398894/150‑1 (MQ=255)
                    tCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGGCTCTGCCCttttg                                                                                                                            <  1:35221/150‑4 (MQ=255)
                     cGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                           <  1:188171/150‑1 (MQ=255)
                     cGATAGGTGTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                           >  2:22427/1‑150 (MQ=255)
                      gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcag                                                                                                                          <  3:142312/150‑1 (MQ=255)
                          gTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttat                                                                                                                      <  2:380145/150‑1 (MQ=255)
                           ttttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatc                                                                                                                     >  3:271773/1‑150 (MQ=255)
                            tttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAACAGGGCAGAGCCAGTGGCCCTGCCCgtatcagttatca                                                                                                                    >  3:258872/1‑150 (MQ=255)
                                 tgtgCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACACGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCt                                                                                                               <  4:151406/150‑1 (MQ=255)
                                     cAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAgg                                                                                                           <  2:324630/150‑1 (MQ=255)
                                        gTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATCGGCAAGGGGCAGGCGCCGCGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTc                                                                                                        <  2:18604/150‑1 (MQ=255)
                                         ttttCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                       <  3:27197/150‑1 (MQ=255)
                                         ttttCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                       <  3:43493/150‑1 (MQ=255)
                                                           cACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGt                                                                                     <  2:97535/150‑1 (MQ=255)
                                                            aCGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTa                                                                                    <  2:119232/150‑1 (MQ=255)
                                                                          gACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCa                                                                      >  4:235805/1‑150 (MQ=255)
                                                                               ggCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACgcgg                                                                 <  1:325838/150‑1 (MQ=255)
                                                                                           gCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGAtt                                                     <  1:22427/150‑1 (MQ=255)
                                                                                              tttGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCgg                                                  <  4:214423/150‑1 (MQ=255)
                                                                                              ttagATAATCATTGTATCCGCTGATTCGTTACGGTGAGAATGGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGTCCTCAGTTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCgg                                                  <  2:394461/147‑1 (MQ=255)
                                                                                                 gATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGgcgc                                               <  3:227410/150‑1 (MQ=255)
                                                                                                   tAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCGTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGACCCGGTACCGGGGGTACCACTGCGCCTTCGCGGGGCGCATCAGGATTCGGCGCTg                                             <  2:192140/150‑1 (MQ=255)
                                                                                                              tCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcatc                                  <  4:53231/150‑1 (MQ=255)
                                                                                                                        cGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCa                        <  4:271773/150‑1 (MQ=255)
                                                                                                                         gTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAg                       >  1:283223/1‑150 (MQ=255)
                                                                                                                                        ttataaGGGCAGGGCCACTGGCTCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGttt        >  2:72498/5‑150 (MQ=255)
                                                                                                                                          aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCa      <  3:141333/150‑1 (MQ=255)
                                                                                                                                           taaGGGCAGGGCCACTGGCTCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg     >  1:59805/2‑150 (MQ=255)
                                                                                                                                              gggCAGGGCCACTGGCTCTGCCCTTATCAGTGATCAGGCTTCAGGTTCCTGAGCCGGTACCGGGGGTACCGCTAGTCTTTCGCTCGGCTCGTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcg  >  1:382463/1‑150 (MQ=255)
                                                                                                                                                gCAGGGCCACTGGCTCTGCCCTTTTCAGTTATCAGGCCTCAGGTTCCTGATc                                                                                                  >  2:81926/1‑52 (MQ=18)
                                                                                                                                                gCAGGGCCACTGGCTCTGCCCTTTTCAGTTATCAGGCCTCAGGTTCCTGATc                                                                                                  <  1:81926/52‑1 (MQ=18)
                                                                                                                                                    |                                                                                                                                               
TCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCG  >  NC_000913_3_hsa_tpiA/2432837‑2433128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑