Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,027,740 G→A L345L (CTG→TTG)  fadA ← 3‑ketoacyl‑CoA thiolase (thiolase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,027,7400GA98.5% 210.0 / NA 68L345L (CTG→TTG) fadA3‑ketoacyl‑CoA thiolase (thiolase I)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (32/35);  minor base C (0/1);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAA  >  NC_000913_3_hsa_tpiA/4027599‑4027882
                                                                                                                                             |                                                                                                                                              
cGGCAAGTGGTTAAACCCGATCAAACACCGTCGCAATGCCCTGACCCTGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGa                                                                                                                                         <  2:700460/149‑1 (MQ=255)
  gCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGAcc                                                                                                                                       <  1:760604/149‑1 (MQ=255)
      gTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAgc                                                                                                                                   <  1:283156/149‑1 (MQ=255)
          ttAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGGTGGCCCAGCGCGa                                                                                                                               >  2:171309/1‑149 (MQ=255)
              aCCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGACTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCACCGCGCTccc                                                                                                                           >  2:337763/1‑147 (MQ=255)
               cccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACg                                                                                                                                              <  2:789972/129‑1 (MQ=255)
               cccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACg                                                                                                                                              >  1:789972/1‑129 (MQ=255)
               cccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATcgcg                                                                                                                          >  1:749015/1‑149 (MQ=255)
                ccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGAt                                                                                                                              <  2:689613/144‑1 (MQ=255)
                ccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGAt                                                                                                                              >  1:689613/1‑144 (MQ=255)
                ccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCgcgc                                                                                                                         <  2:802914/149‑1 (MQ=255)
                ccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCgcgc                                                                                                                         >  2:771700/1‑149 (MQ=255)
                      aaaCACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGt                                                                                                                   >  2:687224/1‑149 (MQ=255)
                       aaCACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCCtt                                                                                                                  >  2:104877/1‑149 (MQ=255)
                          acCGTCGTAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAg                                                                                                               <  2:150876/149‑1 (MQ=255)
                           ccctctcAATACCCGCACCCAGACCGATACCCATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCATCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAgg                                                                                                              <  2:655396/143‑1 (MQ=255)
                           ccGTCACAATACCCTGGCCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAgg                                                                                                              <  1:65800/149‑1 (MQ=255)
                             gTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGtt                                                                                                            >  2:554238/1‑149 (MQ=255)
                              tCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTg                                                                                                           <  1:463589/149‑1 (MQ=255)
                               cGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGa                                                                                                          <  2:160167/149‑1 (MQ=255)
                                     ccccTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGAtcttct                                                                                                    <  1:235856/148‑1 (MQ=255)
                                      cccTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCACCGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTtctc                                                                                                   <  1:834414/149‑1 (MQ=255)
                                      cccTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTtctc                                                                                                   <  1:270389/149‑1 (MQ=255)
                                      cccTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGAGGTGCTGATACGCGCCCCCGAACCACCCAACGGAACACCCAGCGCCACCCCCCCACCCTTGAGGCTGaccctccc                                                                                                   >  2:320996/1‑142 (MQ=255)
                                             ccAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGGTGAGGTTGATCTTCTCGTCAATc                                                                                            >  1:216409/1‑149 (MQ=255)
                                              cAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAATCCAACGGATGACCCAGCGCGATCGCGCCACCGTGGAGGTTGATCTTCTCGTCAATCt                                                                                           >  1:189553/1‑149 (MQ=255)
                                               aGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTg                                                                                          >  1:693691/1‑149 (MQ=255)
                                                       acacAGCGTCGCCAGACCAAACTGAACGGCTTTGCGTTCTATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTa                                                                                  <  2:266297/149‑1 (MQ=255)
                                                         acaTCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGt                                                                                >  1:603654/1‑149 (MQ=255)
                                                          caTCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTc                                                                               >  1:769373/1‑149 (MQ=255)
                                                           aTCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCAATCAGATTCAGCAGCGTGGTGATGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCGCCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTcc                                                                              >  1:282835/1‑149 (MQ=255)
                                                                 gCCAGACCGAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGAGACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATc                                                                        <  1:748012/149‑1 (MQ=255)
                                                                 gCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCACACCGGACCAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCCCGTCAATCTGCTCAATTAGTCCCAGTTc                                                                        >  2:377793/1‑149 (MQ=255)
                                                                   cAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCtt                                                                      <  2:705769/149‑1 (MQ=255)
                                                                       ccAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAAt                                                                  <  2:336708/149‑1 (MQ=255)
                                                                         aaaCTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTGCCAGATCTTTAATAc                                                                <  2:693691/149‑1 (MQ=255)
                                                                         aaaCTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGCTGATGGTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATAc                                                                >  1:235293/1‑149 (MQ=255)
                                                                          aaCTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACa                                                               <  1:163834/149‑1 (MQ=255)
                                                                           aCTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACAt                                                              <  2:548225/149‑1 (MQ=255)
                                                                           aCTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACAt                                                              <  1:536336/149‑1 (MQ=255)
                                                                             tGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGa                                                                          <  1:467860/135‑1 (MQ=255)
                                                                             tGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGa                                                                          >  2:467860/1‑135 (MQ=255)
                                                                             tGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTACTCCCAGACCTTTAATACTTgc                                                            >  2:724652/1‑148 (MQ=255)
                                                                               aaCGTCTTTGCGTTCCATCAGATTCAACAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCa                                                          <  1:375606/149‑1 (MQ=255)
                                                                                   tCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGAt                                                      >  2:51009/1‑149 (MQ=255)
                                                                                    cTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATc                                                     >  2:597275/1‑149 (MQ=255)
                                                                                    cTTTGCGTTCCATCAGATTCAGCAGCGTGCTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATc                                                     >  2:764026/1‑149 (MQ=255)
                                                                                         cGTTGCATCAGATTCAGCAGCGTGGTGCTGTTACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTgcgc                                                <  2:282835/149‑1 (MQ=255)
                                                                                          gTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGAt                                                      >  1:333731/1‑142 (MQ=255)
                                                                                          gTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGAt                                                      <  2:333731/142‑1 (MQ=255)
                                                                                           ttCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCgg                                              <  1:144267/149‑1 (MQ=255)
                                                                                           ttCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCgg                                              >  1:249122/1‑149 (MQ=255)
                                                                                               accaaaTTCACCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAACACCTGGCAGGATCTGCGCGGCaaa                                          <  2:316297/144‑1 (MQ=255)
                                                                                               aTCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCaaa                                          <  2:749015/149‑1 (MQ=255)
                                                                                                tCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGAGCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAg                                         >  1:36207/1‑149 (MQ=255)
                                                                                                tCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAg                                         >  1:356187/1‑149 (MQ=255)
                                                                                                tCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAg                                         >  1:424201/1‑149 (MQ=255)
                                                                                                        agcagcGTGGTGCTGATACGCGCACCGGAACAACCCAACGGCTCATGCCTCTCTATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGtt                                 <  1:195042/149‑1 (MQ=255)
                                                                                                         gcagcGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTc                                <  2:672706/149‑1 (MQ=255)
                                                                                                           agctcggtggtGATACGCTGACCTGAACACGCCCACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCACGTCGTCCGGTCCAATCAGGCCCAGACCCTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCAt                              <  2:181652/144‑1 (MQ=255)
                                                                                                           agcGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCATTTCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCAt                              <  1:191067/149‑1 (MQ=255)
                                                                                                             cGTGGTGCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCAttt                            <  2:356187/149‑1 (MQ=255)
                                                                                                                 gtgCTGATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCaaa                        <  2:249122/149‑1 (MQ=255)
                                                                                                                      gATACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGc                   >  1:734192/1‑149 (MQ=255)
                                                                                                                       aTACGCGCACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGcc                  >  2:122546/1‑149 (MQ=255)
                                                                                                                             gcACCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTCGTCCCAGATTGTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATc            <  2:603654/149‑1 (MQ=255)
                                                                                                                               aCCGGAACAACCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGc          >  2:689652/1‑149 (MQ=255)
                                                                                                                                       aacCCAACGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGaa  >  1:102448/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
CGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAA  >  NC_000913_3_hsa_tpiA/4027599‑4027882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑