Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA59,0890AC57.9% 2.2 / 19.9 19pseudogene (38/228 nt)yabPpseudogene, pentapeptide repeats‑containing
Reads supporting (aligned to +/- strand):  ref base A (5/3);  new base C (8/3);  total (13/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGA  >  NC_000913_3_hsa_tpiA/58958‑59238
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gCGTTGACATTAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCcggacggtgctaccccggacggtgctaaccc                                                                                                                                     <  1:202700/150‑2 (MQ=255)
        aTGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGTTg                                                                                                                             >  2:383923/1‑150 (MQ=255)
        aTGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTg                                                                                                                             <  2:221793/150‑1 (MQ=255)
         tGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGGGc                                                                                                                            >  2:125162/1‑150 (MQ=255)
          gAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCt                                                                                                                           >  1:303226/1‑150 (MQ=255)
            gggATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGCCGGTGCTACCCCGGACGGTGCTAATCCGGACCGTGCTaa                                                                                                                         >  1:15425/1‑149 (MQ=255)
               aTTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTaacct                                                                                                                      <  1:223746/150‑5 (MQ=255)
                       cTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTg                                                                                                              >  1:254224/1‑150 (MQ=255)
                            tAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTGcc                                                                                                         >  1:417313/1‑150 (MQ=255)
                                 aaaCAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGCGCTAACCCGGACGGTGCTCACCTGGACGGTGCTAccgtgaa                                                                                                    >  1:207015/1‑145 (MQ=38)
                                  aaCAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTAccgtgaac                                                                                                   >  1:506960/1‑144 (MQ=38)
                                   aCAAGGAAAGGTAGCAAAGATATCGTCTATGAGAGGCCTGGGTGGGATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGAAGGTGCTAccgtgaacg                                                                                                  <  2:279687/150‑8 (MQ=21)
                                                 cAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCcggacggtgctaccccggacggtgctaccc                                                                                                                                                     <  2:122558/85‑1 (MQ=255)
                                                                                     tAACCTGGGCGTGCTACCCcggacggtgctaccccggacggtgctaccc                                                                                                                                                     >  1:491516/1‑49 (MQ=255)
                                                                                     tAACCTGGGCGTGCTACCCcggacggtgctaccccggacggtgctaccc                                                                                                                                                     <  2:491516/49‑1 (MQ=255)
                                                                                         cggacggtgctaccccggacggtgctaccccggacgGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAg                                            >  1:442307/7‑150 (MQ=255)
                                                                                         cggacggtgctaccccggacggtgctaccccggacgGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAg                                            >  1:697481/7‑150 (MQ=255)
                                                                                                   taccccggacggtgctaccccggacggtgcTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGAt                                                   <  1:406550/133‑1 (MQ=255)
                                                                                                   taccccggacggtgctaccccggacggtgcTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGAt                                                   >  2:406550/1‑133 (MQ=255)
                                                                                                          gacggtgctaccccggacggtgctaACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTgaagaa                           <  2:303226/150‑1 (MQ=255)
                                                                                                                  taccccggacggtgctaACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGctac                   >  1:224072/1‑150 (MQ=255)
                                                                                                                    ccccggacggtgctaACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGcta                    <  1:352982/147‑1 (MQ=255)
                                                                                                                    ccccggacggtgctaACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGcta                    >  2:352982/1‑147 (MQ=255)
                                                                                                                     cccggacggtgctaacccggacggtgctaaCCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattattatt                                                                              >  1:331527/1‑88 (MQ=255)
                                                                                                                     cccggacggtgctaacccggacggtgctaaCCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattattatt                                                                              <  2:331517/88‑1 (MQ=255)
                                                                                                                     cccggacggtgctaacccggacggtgctaaCCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattattatt                                                                              <  2:331527/88‑1 (MQ=255)
                                                                                                                     cccggacggtgctaacccggacggtgctaaCCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAattattatt                                                                              >  1:331517/1‑88 (MQ=255)
                                                                                                                     cccggacggtgctaacccggacggtgctaaCCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTaa                >  1:421225/1‑150 (MQ=255)
                                                                                                                              tgctaacccggacggtgctaaacTGGACGGTGCTACAGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTAct       >  2:616008/1‑150 (MQ=255)
                                                                                                                                   acccggacgggtctaatcTGTACGGTGCTACCGTGAACGGTTCTACCTACATATATGATGAGGTAATTATTATTAATAAAATCGCCCGCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGa  <  1:454694/150‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                                     
GCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGA  >  NC_000913_3_hsa_tpiA/58958‑59238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑