Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,817,170	C→A	E112* (GAA→TAA)	yqaB ←	fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,817,170	0	C	A	100.0%	67.6 / NA	23	E112* (GAA→TAA)	yqaB	fructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/14);  total (9/14)

CGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCT  >  NC_000913_3_hsa_tpiA/2817043‑2817316                                                                                                                                |                                                                                                                                                    cGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                               <  1:27980/150‑1 (MQ=255)   cTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTgg                                                                                                                             <  1:496979/150‑1 (MQ=255)    ggcgcgcACAACAAAAAGGTGTCTGGAGCGGGGTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTggg                                                                                                                            <  2:636533/149‑1 (MQ=255)                    aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCCACG‑ACCATGCCAAc                                                                                                           >  2:372837/1‑150 (MQ=255)                        gtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACtttt                                                                                                       <  1:571262/150‑1 (MQ=255)                         tCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTc                                                                                                      <  1:424814/150‑1 (MQ=255)                                cgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACAt                                                                                               >  2:561056/1‑150 (MQ=255)                                 gggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATc                                                                                              <  2:86062/150‑1 (MQ=255)                                        tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACCaga                                                                                       >  1:79294/1‑150 (MQ=255)                                         ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAagag                                                                                      >  1:697246/1‑150 (MQ=255)                                         ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAagag                                                                                      <  1:157264/150‑1 (MQ=255)                                                      aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                         <  2:133470/150‑1 (MQ=255)                                                      aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCA‑TCGTTTACGCGCCGTGCCACCTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                         >  1:159068/1‑150 (MQ=255)                                                                 cgagtgCGTGAAAATAAGGGCGTGAGCCCATGGCCGCCATCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                              <  2:563879/145‑1 (MQ=255)                                                                             aagaatGGCGTAATCCTAGGTGCGCCAGCAATGTCTCAGCGAGGGCGCTTTACCTCCCCGTTCCTACAGCCATTGGGTGACG‑ACCATGCCAACGTTCCACCGCATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtcttc                                                  <  2:497081/147‑2 (MQ=255)                                                                              agaatGGCGAAATCCCAGGGGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTTCTACAGCCATTGGGCGACG‑ACCATGCCAACCTTTCTCCACATCAACAAGAGTAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAc                                                 <  2:289798/148‑1 (MQ=255)                                                                                aatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACACGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg                                               <  1:518408/150‑1 (MQ=255)                                                                                   ggCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCGCTCCGTCGACAAGAGGAAGCGGGTGGACGCTATCCAGCAGCATACTTCTTACTGCtt                                            >  1:512836/1‑150 (MQ=255)                                                                                    gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTCTCCCGCAGCATACTTCTTACTGCTTc                                           >  1:88576/1‑150 (MQ=255)                                                                                           cccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttttt                                    <  1:674052/150‑1 (MQ=255)                                                                                                gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCCCATCAACAAGATGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcg                               <  2:79294/150‑1 (MQ=255)                                                                                                                tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATCCTTCTTACTGCTTCTGTTTTTTCACGCCCTAACCCCTGCggg               >  2:262045/1‑150 (MQ=255)                                                                                                                             tttAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCt  >  1:269344/1‑150 (MQ=255)                                                                                                                                |                                                                                                                                                    CGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCT  >  NC_000913_3_hsa_tpiA/2817043‑2817316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑