New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 4347349 =28 (0.810)4 (0.120) 4/266 4.3 12.7% intergenic (‑41/+37) fumB/dcuB anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
?NC_000913_3_hsa_tpiA 4347377 = 29 (0.900)intergenic (‑69/+9) fumB/dcuB anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGA  >  NC_000913_3_hsa_tpiA/4347242‑4347521
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gATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCCCGGGCGGCACGCAAAGGGCATTtata                                                                                                                                     >  1:405526/1‑149 (MQ=255)
  tAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATGTATAAg                                                                                                                                   >  1:225783/1‑149 (MQ=255)
     tATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAAc                                                                                                                                <  1:171440/149‑1 (MQ=255)
          ggTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTa                                                                                                                           >  2:275166/1‑149 (MQ=255)
                   tttCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGGCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCgcggc                                                                                                                  <  1:390784/149‑1 (MQ=255)
                        ccATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACGCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGAGCATTTATAACAACCCGTACACCGCGGCGAAGa                                                                                                             >  2:235127/1‑149 (MQ=255)
                         cATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGAt                                                                                                            >  2:110574/1‑149 (MQ=255)
                                aaaGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGGGGGCGGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCg                                                                                                     <  2:582452/149‑1 (MQ=255)
                                               aTAAAGGGTTTGTTTGACATAGCTTCCAGACTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCCGCCACGATGGAGACGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGAACGATACg                                                                                      >  1:140384/1‑149 (MQ=255)
                                                tAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCGGTATTTTTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGACCCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGc                                                                                     <  1:104820/149‑1 (MQ=255)
                                                 aaaGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTAGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCt                                                                                    <  2:534219/149‑1 (MQ=255)
                                                   aGGGTTTGTTTGCCATTGTTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCTGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTca                                                                                  <  2:22754/149‑1 (MQ=255)
                                                                  tAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCg                                                                   >  2:380317/1‑149 (MQ=255)
                                                                                 aaCTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtgt                                                    <  2:549708/149‑1 (MQ=255)
                                                                                   ctctGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAg                                                                                                        <  2:262134/95‑1 (MQ=255)
                                                                                   ctctGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAg                                                                                                        >  1:262134/1‑95 (MQ=255)
                                                                                        tagttGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgat                                             <  2:542373/146‑1 (MQ=255)
                                                                                          tttGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatga                                           <  2:201416/149‑1 (MQ=255)
                                                                                              ttATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGaa                                       <  1:275166/149‑1 (MQ=255)
                                                                                                aTTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAGAGCGGGGGTTGGTGACGAAGc                                     >  1:135084/1‑149 (MQ=255)
                                                                                                     aaaaTGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCg                                >  1:142740/1‑149 (MQ=255)
                                                                                                            cGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAAGAAAGCAGTGGTTGATGACGAAGCGCCCGAGgtggg                         >  2:465185/1‑149 (MQ=255)
                                                                                                                 cgcgTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGgtggtg                    >  1:211514/1‑149 (MQ=255)
                                                                                                                        tCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTa                                                                                                                           <  1:541179/39‑1 (MQ=255)
                                                                                                                        tCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTa                                                                                                                           >  2:541179/1‑39 (MQ=255)
                                                                                                                        tCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACGTCGCGGCGAAGATCCAGCGGAAGACGCACGATACGCTCACACAAATCAACCCCGGCAGAATAAAGGTGTGGTTGATGACGAAGCGCACGATGTGGGTGGTGACGGaag             >  2:260996/1‑148 (MQ=255)
                                                                                                                        tCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGCCGCACGGTACGCTCACACCAATCAACCCCGGCAGCATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGGGCCGGAAc             >  1:237026/1‑149 (MQ=255)
                                                                                                                            gggCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTc         <  1:178493/149‑1 (MQ=255)
                                                                                                                            gggCGGCACGCAAAGTGCATATATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACACACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCAGTGGTTGATGACGAAGCGACCAATGTGGGTGGTGCCGGAAAGGTc         >  2:12618/1‑149 (MQ=255)
                                                                                                                               cggcACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGGTCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCaaa      <  2:431605/149‑1 (MQ=255)
                                                                                                                                   aCGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGa  >  2:380583/1‑149 (MQ=255)
                                                                                                                                       |                                                                                                                                                
GATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGA  >  NC_000913_3_hsa_tpiA/4347242‑4347521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.