Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA482,2300GA98.1% 170.4 / NA 54P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/26);  minor base C (1/0);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGC  >  NC_000913_3_hsa_tpiA/482084‑482368
                                                                                                                                                   |                                                                                                                                          
tgaaatAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGGGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCagg                                                                                                                                          <  1:259105/149‑1 (MQ=255)
   catAAACTTTCTTCACACGGCCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCTTGGTCGATATCAATCTTAAACTGCAGGGTa                                                                                                                                       <  2:612542/149‑1 (MQ=255)
       aaCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCtt                                                                                                                                   >  1:397863/1‑149 (MQ=255)
        aCTTTCTTCAGACGTCCGCGGTGGATTAAGTCGTTCCCTTAGCTGCCGCCCCAAGCATCGCCCAGAGTGGCGTTCTTGTCGTCG‑ATATAAACACCCAGCGCCTGCGCTTTGTCCTGGTCGATATCATTCTTGCATTGCTGGGTCTCTTc                                                                                                                                  >  2:678347/1‑149 (MQ=255)
         cTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTcc                                                                                                                                 <  2:397863/149‑1 (MQ=255)
          tttCTTCACACGACCCCGGTACATAATGTTGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTC‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGACTATATCAATCTTTAACTGCAGGGTATCTTCCa                                                                                                                                <  1:86445/149‑1 (MQ=255)
                 acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGGGAGGGGTTCCTGTCGTTG‑ATAGAAACACCCACCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTACCTTCCTGACCGtg                                                                                                                         >  2:411062/1‑148 (MQ=255)
                 acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCCGGGTATCTTCCAGACCGtt                                                                                                                         >  1:354519/1‑149 (MQ=255)
                 acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                         >  1:372809/1‑149 (MQ=255)
                            gTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGAGACCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGc                                                                                                              >  1:444813/1‑149 (MQ=255)
                               gATAAAGTCGTTCACATAGCCGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCGTGGCCTGTGCTTTTTCCTGGTCGATATCAATCTTAAACTCCAGGGTATCTTCCAGACCGTTGGGACGTACCCTgg                                                                                                           >  2:256406/1‑149 (MQ=255)
                                   aaGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCaa                                                                                                       <  2:330616/149‑1 (MQ=255)
                                   aaGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCaa                                                                                                       <  2:332672/149‑1 (MQ=255)
                                    aGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCCGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCCATTTTTCCTGGTCTATATCAATCTTAACCTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                      <  1:233518/149‑1 (MQ=255)
                                      ttgTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACat                                                                                                    <  1:323575/147‑1 (MQ=255)
                                        gTTCACATAGCTGCCGCCCCATGCAGCGCGCAGAGTGGTGTTAATGAAGTAG‑ATAGACACACCCAGCGCCTGCACTTTTTCCTGGTAGATATCAATCTTAAACAGCAGGGTATCTTCCAGACCGTCTGGACGTACGCTGGTCAACatat                                                                                                  >  1:635648/1‑149 (MQ=255)
                                         ttCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATc                                                                                                 >  1:632631/1‑149 (MQ=255)
                                          tCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCa                                                                                                <  2:235233/149‑1 (MQ=255)
                                            acaTAGCTGCCGCCCCATGCAGCGCCCTGATGGCTTTTCATGTCGTTC‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCCATCTTAACCTGCATGGTATCCTTCATGCCGTTTGGTCGTACGCTGGTCAACATTTCCgg                                                                                              <  2:344488/149‑1 (MQ=255)
                                            acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                              <  2:531681/149‑1 (MQ=255)
                                               tAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTg                                                                                           >  1:32782/1‑149 (MQ=255)
                                                  cTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTTGTCAACATATCAGGGTGCtt                                                                                        <  2:225174/149‑1 (MQ=255)
                                                   tGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGATGGTAAACATAACAGGGTGCtgc                                                                                       >  1:216368/1‑147 (MQ=255)
                                                     gccccccATGCAGCGCTCGTATTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgc                                                                                     <  2:401582/146‑1 (MQ=255)
                                                       gccCCATGCAGCGCCCAGAGTGGTGTTAATCTCGTTGTATA‑AAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATAACCGGGTGCTTCgctg                                                                                   >  1:369075/1‑149 (MQ=255)
                                                         cccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGCCCGTTTGGACGTACGCTGATCAACATATCAGGGGGCTTCgctcct                                                                                 >  1:257666/1‑149 (MQ=255)
                                                             tGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTg                                                                             >  2:532463/1‑149 (MQ=255)
                                                               caacgcCCAGTTGGGTGTTAACGTCGTTT‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgca                                                                           <  1:169805/146‑1 (MQ=255)
                                                                cgcgcCCAGTGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                          <  1:697805/148‑1 (MQ=255)
                                                                      cAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCCACt                                                                    >  2:237531/1‑149 (MQ=255)
                                                                      cAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGAACGTCCGCTGGTCAACATATCATGGTGCTTCGCTGCTTCTccacgcactc                                                                    >  2:415525/1‑146 (MQ=255)
                                                                                ttAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGAGCCTAGGGTCGAGCTCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACgcgc                                                          >  1:551768/1‑149 (MQ=255)
                                                                                    tGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                      <  2:365181/149‑1 (MQ=255)
                                                                                        gTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                  <  2:109439/149‑1 (MQ=255)
                                                                                             aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCCGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                              >  1:209380/1‑149 (MQ=255)
                                                                                                gAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                           <  2:372809/149‑1 (MQ=255)
                                                                                                 aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCCAGCAACTGGTTCCGCGCCTGAGTCAGTTTTTCg                                          >  2:164615/1‑149 (MQ=255)
                                                                                                  aaCTCCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                         <  2:235863/149‑1 (MQ=255)
                                                                                                  aaCCCCCCGCGCCTGCGCTTTTTCCGTGTCGATATCAATCTTACACTGCAGTGTATCTCCCAGCCCGTTTGGACGTACGCTGGTCAACATCTCAGGGTGCTTCGCTGCTTCTGCAAGCAACCGGTTACGCGCCTTAGTCAGTTTTTCGt                                         <  1:411062/149‑1 (MQ=255)
                                                                                                            gcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGcc                               >  2:328145/1‑149 (MQ=255)
                                                                                                             ccTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                              >  1:591584/1‑149 (MQ=255)
                                                                                                                  gcTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                           >  1:190501/1‑131 (MQ=255)
                                                                                                                  gcTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                           <  2:190501/131‑1 (MQ=255)
                                                                                                                    tttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCCGCGTCCTTCGCCGCTTCTGCAAGCCACTGCTTACGCCCCTTAGTCAGTTTTTCGTGACCAAGGCCATCCTGGt                       <  2:444813/149‑1 (MQ=255)
                                                                                                                     ttttCCTGGTCGATATCAATCTTCAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTc                      <  2:199823/149‑1 (MQ=255)
                                                                                                                           tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                >  1:11695/1‑149 (MQ=255)
                                                                                                                             gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt              >  2:47588/1‑149 (MQ=255)
                                                                                                                                  tatCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGTCTGGTCAATCAGCtcaaag         <  2:632631/149‑1 (MQ=255)
                                                                                                                                   atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAAGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCCGCtcaaagt        >  1:664689/1‑149 (MQ=255)
                                                                                                                                    tCAATCTTAAACTGCAGGGTATCTTCCAGCACGAAGGCACGGACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc       >  1:743059/1‑149 (MQ=255)
                                                                                                                                    tCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc       >  1:600095/1‑149 (MQ=255)
                                                                                                                                     cAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca      >  1:61775/1‑149 (MQ=255)
                                                                                                                                       aTCTTAAACGGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaa    <  1:699734/149‑1 (MQ=255)
                                                                                                                                        tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag   <  1:117982/149‑1 (MQ=255)
                                                                                                                                         cTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc  <  2:223056/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTG‑ATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGC  >  NC_000913_3_hsa_tpiA/482084‑482368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑