Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA482,2300GA98.0% 164.7 / NA 51P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/27);  minor base C (1/0);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTT  >  NC_000913_3_hsa_tpiA/482084‑482373
                                                                                                                                                  |                                                                                                                                               
tgacatAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCagg                                                                                                                                               <  2:86630/149‑1 (MQ=255)
  acatAAACTTTCTTCGCTTGACCGCGGTCGGTAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGTCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGTAGGGt                                                                                                                                             <  2:744079/149‑1 (MQ=255)
     tAAACTTTCTTCAAACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATc                                                                                                                                          <  1:218603/149‑1 (MQ=255)
        cctgtggtcACACGCGCGCGGGCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGGCGTTGATAGAAACACGCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                       <  2:545065/142‑1 (MQ=255)
        aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                       <  1:803364/149‑1 (MQ=255)
        aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                       >  1:582349/1‑149 (MQ=255)
          tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGGGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                     <  2:321541/149‑1 (MQ=255)
                cacaCGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGt                                                                                                                               >  2:373541/1‑149 (MQ=255)
                 acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGCTATCAATCTTGAACTGCCGGGTAGCTTCCAGACCGtt                                                                                                                              >  1:397746/1‑149 (MQ=255)
                 acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCGGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                              >  1:330060/1‑149 (MQ=255)
                          cgGTCGATAAAGTCGTTCCCATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAc                                                                                                                     <  1:472834/149‑1 (MQ=255)
                                    aGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATGAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                           <  2:230671/149‑1 (MQ=255)
                                          gcgcaTAGCTGCCTCTGCATGTATGGCCAAGATTGGTGTAAATGTCGTTGATAGAAACACCCATCGCCTGCGCTTTTTCCTGGTCTATATCAATCTTAAACTGCAGGGTATCTTCCAGACGGTTTGTACGTACGCTGGTCAACATATCa                                                                                                     <  2:788338/146‑1 (MQ=255)
                                             caTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                  <  2:575772/149‑1 (MQ=255)
                                             caTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                  <  2:234755/149‑1 (MQ=255)
                                                aGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCa                                                                                                     <  2:448665/143‑1 (MQ=255)
                                                aGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGGCAACATATCa                                                                                                     >  1:448665/1‑143 (MQ=255)
                                                                aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                               <  1:877551/149‑1 (MQ=255)
                                                                aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                               <  1:151866/149‑1 (MQ=255)
                                                                             gtgtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgctg                                                                                        >  2:307796/1‑127 (MQ=255)
                                                                             gtgtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgctg                                                                                        <  1:307796/127‑1 (MQ=255)
                                                                                ttAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCCATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAAGTACGCTGGTCAACATATAAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgcga                                                               >  1:682633/1‑148 (MQ=255)
                                                                                    tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                           <  2:493753/149‑1 (MQ=255)
                                                                                      tCGTTGATAGTAATACCCAGCGCCTGCGCTTTTTCCTCGTCGATATCAATCTTAACCTGCAGGGTATCTTCCAGCCCGTTTGGACGTACACTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCACGCAACGGGTTACCCGCCTGact                                                         >  2:885551/1‑147 (MQ=255)
                                                                                        gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                       >  1:542063/1‑149 (MQ=255)
                                                                                         ttGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                 <  2:94441/106‑1 (MQ=255)
                                                                                         ttGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                 >  1:94441/1‑106 (MQ=255)
                                                                                           gATAGAAACACCCAACGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAAGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTTCAATCAACTGGTTACGCGCCTAACTCAGtt                                                    >  2:382188/1‑149 (MQ=255)
                                                                                            aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                   >  2:562762/1‑149 (MQ=255)
                                                                                               gAAACACGCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGCACGCGGGTCAACATATCAGGGTGCTTCGCTGCCTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                <  2:639889/149‑1 (MQ=255)
                                                                                               gAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                <  2:582349/149‑1 (MQ=255)
                                                                                                 aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                              <  1:373541/149‑1 (MQ=255)
                                                                                                   cacCCAGCGCCTGCGCTTTTTCTTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa                                            <  2:682989/149‑1 (MQ=255)
                                                                                                             cTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAg                                  >  1:739391/1‑149 (MQ=255)
                                                                                                              tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                  <  2:76416/84‑1 (MQ=255)
                                                                                                              tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                  >  1:76416/1‑84 (MQ=255)
                                                                                                              tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGc                                 >  2:744401/1‑149 (MQ=255)
                                                                                                               gcgcTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATCTCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAACGCCAGcc                                >  2:518131/1‑149 (MQ=255)
                                                                                                               gcgcTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCTGcc                                >  2:958951/1‑149 (MQ=255)
                                                                                                                 gcTTTTTCCTGGTCGATATCAATCGTAAACTGCAGGGTATCTTCCAGACCGTCTGGACGTACGCTGGTCACCATATCAGGGTGCTTCGCTGCTGCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTGGTGACCAAGGCCAGCCTg                              <  1:885551/149‑1 (MQ=255)
                                                                                                                  cattttCCTGGTCGATATCAATCTTAAACGGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTgg                             >  1:196465/3‑149 (MQ=255)
                                                                                                                  cTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTgg                             <  1:880417/149‑1 (MQ=255)
                                                                                                                          tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAAGCGCGGGTTTCGGTTTGTGTTGACCAAGGCCAGCCTGGTCAATCAg                     >  1:267807/1‑149 (MQ=255)
                                                                                                                            gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                   <  2:739391/149‑1 (MQ=255)
                                                                                                                                atatCAATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                              >  1:792149/1‑38 (MQ=255)
                                                                                                                                atatCAATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                              <  2:792149/38‑1 (MQ=255)
                                                                                                                                   tCAATCTTAAACTGCAGGGTATCTTCCAGACTGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc            >  2:928761/1‑149 (MQ=255)
                                                                                                                                      aTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaa         <  1:562762/149‑1 (MQ=255)
                                                                                                                                       tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTCCGCGCGTGATGCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag        >  1:707215/1‑149 (MQ=255)
                                                                                                                                         ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAGAGcg      >  1:97142/1‑148 (MQ=255)
                                                                                                                                         ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc      >  2:261627/1‑149 (MQ=255)
                                                                                                                                             aatGCAGGGTATCTTCCAGACCGTCTGGACGTACGCTGGTCAACATAACAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACAAAGGCAAGACTGGTAAATCAGCTCAAAGTCAAAGCCGGtt  <  1:286145/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTT  >  NC_000913_3_hsa_tpiA/482084‑482373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑