Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,817,1700CA96.8% 83.9 / NA 32E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (14/16);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAT  >  NC_000913_3_hsa_tpiA/2817024‑2817310
                                                                                                                                                  |                                                                                                                                            
cGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAAc                                                                                                                                            <  1:282568/149‑1 (MQ=255)
 gTCGGTTGCACGCCCATACGCTGCACGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATACTGGCGTAATCCCAGGTGCGCCCGCAATGCCTCAGCGATGCCGCTCTCACt                                                                                                                                           >  2:113512/1‑149 (MQ=255)
         cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                   >  2:364507/1‑149 (MQ=255)
          aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCATAATCCCAGGAGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTccc                                                                                                                                  >  2:411659/1‑149 (MQ=255)
           aggtcATACCCTGCGCCAACACCAAAAGTGTGTCTGGCGAGGGCTTATGGTGTTTGACGTGATAGGCAGCGACGACCGCGTCAACATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCt                                                                                                                                 <  1:257875/145‑1 (MQ=255)
              ccATACGCTGCGCGCACAACAAAAATGTGTCCGGCGGGGGATTGTGGTGTTTAACGTGATCAGCACAGACGACGGCGGCAAATTAATAGCGAAACCCCCGGCGAGTCAGCAATGCCTGGGCGCTGGCGCTTTAACTCCCCGTTCCTACa                                                                                                                              >  2:183/1‑149 (MQ=255)
               cATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAg                                                                                                                             >  1:84292/1‑149 (MQ=255)
                  aCGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGAGCGGCAGCGACGACGGCGTCAAAGTAATGGCGTAATCCCAGATGCGCCAGCAATGCATCAGCGATGGCGATTTAACTCCCCGTTCCTACAGCCa                                                                                                                          >  1:527071/1‑149 (MQ=255)
                    ggtGCGCGCACAACAAAAATGTGTCTGGCGTGGGTTTACTGTGTTTGACGTGAGCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCTCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGTCAtt                                                                                                                        <  2:511571/147‑1 (MQ=255)
                     cTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTg                                                                                                                       <  1:35237/149‑1 (MQ=255)
                       gcgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCATGTTCGCCAGCTATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTggg                                                                                                                     <  1:81001/149‑1 (MQ=255)
                               aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc                                                                                                             >  1:165142/1‑149 (MQ=255)
                                  aaaaaTGTGTCAGGCGCGGGTTTATGGTGTTGGACGAGAGCGGCAGCGACGAGGGCGTCACAATAATGGGGAAAGCCCAGGGGCGCCAGCGATGCCGCAGAGAGGGCGCTTTTAGTCCGCCTTCCTACACCCATTGGGagaagagtaag                                                                                                          >  2:511357/1‑144 (MQ=255)
                                   aaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGc                                                                                                         <  1:279068/149‑1 (MQ=255)
                                        gtgtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACt                                                                                                    <  2:202581/149‑1 (MQ=255)
                                            cTGGCGCCGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGTCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTc                                                                                                <  2:472161/149‑1 (MQ=255)
                                             tGGCGCGGGTTTAGGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                               <  2:296311/149‑1 (MQ=255)
                                              ggCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcac                                                                                              >  1:462397/1‑149 (MQ=255)
                                                          tGGTGTTTGACGTGATCGGCAGCGGCGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAag                                                                                  >  1:493980/1‑149 (MQ=255)
                                                           ggTGTTGGACGTGATCCGCAGCGCCGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCCCAGCTGGCTGCTCAACTCCCCGTTCCTACAGCCATGGGGCGACGACCATGCCAACTTGTCACCACCTCAACAaga                                                                                 <  2:260115/149‑1 (MQ=255)
                                                               tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCAAACTTTTCACCACCTCACCAAGAGGaa                                                                             >  2:20366/1‑149 (MQ=255)
                                                                    cGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGt                                                                        <  1:48914/149‑1 (MQ=255)
                                                                                cgacgaCGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGAAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGAAAGCGGTTCGCCGCTATcc                                                            >  1:363642/1‑149 (MQ=255)
                                                                                              aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATCcttct                                              >  1:409513/1‑149 (MQ=255)
                                                                                              aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtct                                              >  1:75618/1‑149 (MQ=255)
                                                                                              aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtct                                              >  1:422903/1‑149 (MQ=255)
                                                                                                            tCCCCGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttt                                <  1:64634/149‑1 (MQ=255)
                                                                                                                  gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGCCGATAGCGAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAc                          <  1:564513/149‑1 (MQ=255)
                                                                                                                          gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAAc                  <  2:325169/149‑1 (MQ=255)
                                                                                                                                    aGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCGGCATACTTCTTACTGCTTCTGTTTTTTCCCGCGCTAACGCATGCGGGt        >  1:31978/1‑149 (MQ=255)
                                                                                                                                       gATGGCGCTTTAAGGACCCGTTCCTACAGCCATTGGGCGACGACCATGCCAATTTTCCATCAGACTAACAAGGGTATGCGGTTCGTCGCTGTTCAGCAGCATACTTCTTACCGCTTCTGTTTTTACACGCGATAACGCATGCGTGTCga     <  2:62808/149‑1 (MQ=255)
                                                                                                                                          ggCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt  <  1:151750/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAT  >  NC_000913_3_hsa_tpiA/2817024‑2817310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑