Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA482,2300GA100.0% 94.4 / NA 29P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/15);  total (14/15)

ACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTT  >  NC_000913_3_hsa_tpiA/482092‑482373
                                                                                                                                          |                                                                                                                                               
accttcttcACACGACGCCGGTCGATAAAGTCGTTCAGATAGCTGCCGCTCCATGCAGCTCCCGGAGTGGTGTTAATGTCGTTGATCTACACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                       <  2:30464/146‑1 (MQ=255)
aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                       >  2:416530/1‑149 (MQ=255)
   ttcttcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCCCAGCCGGGAGTAGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAg                                                                                                                                    >  1:446975/1‑149 (MQ=255)
      ttcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGAcc                                                                                                                                 >  1:202576/1‑149 (MQ=255)
             gACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                          <  1:330395/149‑1 (MQ=255)
             gACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                          <  1:392666/149‑1 (MQ=255)
                         tAAAGTCGTTCACATAGCTGCCGGCCGATGCAGCGACCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTc                                                                                                              <  2:395434/149‑1 (MQ=255)
                         tAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTc                                                                                                              <  2:455872/149‑1 (MQ=255)
                              tCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAAGACCCGGGGCCTTCGCGTTTTCCTGGGCGATATGAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAAGCTGGTCAACat                                                                                                         >  2:232583/1‑149 (MQ=255)
                                 ttCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACAGATc                                                                                                      >  2:205452/1‑149 (MQ=255)
                                    acaTAGCTGCGGCCCCATGCAGCGGCCAGAGGGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGTCCGTTTGGTGGTACGCTGGTCAACATATCAgg                                                                                                   <  2:493146/149‑1 (MQ=255)
                                       tAGCTCCCGCCCTATGCAGCTCCCAGAGGGGTGTTAATGTCGTTGATAGAAACACCCAGCGGCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGGACGCTGGTCAACATATCAGGGTg                                                                                                <  2:412546/149‑1 (MQ=255)
                                                ccccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgctgc                                                                                       >  1:427050/1‑149 (MQ=255)
                                                     tGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTg                                                                                  <  1:386895/149‑1 (MQ=255)
                                                         gcgcCCAGAGTGGTGTTAATGTCGTTGATAGGAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCACTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcagt                                                                              >  1:163225/1‑147 (MQ=255)
                                                              cAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACt                                                                         <  1:407937/149‑1 (MQ=255)
                                                                            tGTCGTTGATAGAACCACCCAGCGCCTGCGCTTTTTCCTGGTCGCTATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                           <  1:326642/149‑1 (MQ=255)
                                                                            tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                           <  1:251516/149‑1 (MQ=255)
                                                                                        aaaCACCCAGCGCCTGCGCTTTTTCCTGGTAGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACCTATCAGGGTGCTTCGCTGCCTCTCCAAGCCACTGGTTAGCCGCCCGACTCACTTTTCCg                                               >  2:444984/1‑149 (MQ=255)
                                                                                              ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTTCGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCACGCAACTGGTTACGCGCCTGAGCCAGTTTCTCGCGACCa                                         >  2:126688/1‑149 (MQ=255)
                                                                                              ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGCTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                         >  1:158572/1‑149 (MQ=255)
                                                                                                 gcgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAgg                                      >  1:78420/1‑149 (MQ=255)
                                                                                                 gcgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTGCCAGACAGTTTGAACGTACGCTGGTCAAAATATCAGGGTGCTTCGCTGCTTCTGCAAGAAACTGGTTACGCGCCGGAGTCAGGTTTTCGTGCCCAAgg                                      >  1:174916/1‑149 (MQ=255)
                                                                                                  cgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                     <  2:38528/149‑1 (MQ=255)
                                                                                                    cgtGCGCTGTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGGCACTGGTTACGCGGCTGAGTCAGGTTTTCGTGACCAAGGCCa                                   >  2:527244/3‑149 (MQ=255)
                                                                                                           tttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                            <  1:435490/149‑1 (MQ=255)
                                                                                                             tttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGtac                          >  1:371658/1‑147 (MQ=255)
                                                                                                                                   aaaCTGCAGGGTATCTTCGAGACCGTTTGGACGTACGCTCGTCAACACATCAGGGTGCTTCGCTGCTTCTGCAAGCAACGGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGTTCAATCAGCTCAAAGTCAAAGCCgg    <  1:432416/149‑1 (MQ=255)
                                                                                                                                     attGCAGGGTATGTTCAAGACCGTTTGGCCGGAGTGTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGGGTCAGTTGTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGttt  <  2:375657/147‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                               
ACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTT  >  NC_000913_3_hsa_tpiA/482092‑482373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑