Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 197,968 G→A 8.5% S14N (AGC→AAC)  bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA197,9680GA 8.5% 119.9 / 3.8 47S14N (AGC→AAC) bamABamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base A (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

TTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATT  >  NC_000913_3_hsa_tpiA/197823‑198116
                                                                                                                                                 |                                                                                                                                                    
tttttATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGTATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTagcag                                                                                                                                                   <  1:475744/146‑1 (MQ=255)
     aTCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCGCTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGACGGCGATGTAAAAGTTGCTCATAGCGTCGATGCTGTTTAGCAGCGCCa                                                                                                                                              >  1:552658/1‑149 (MQ=255)
      tCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGTGGTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTggcagctgcca                                                                                                                                             >  2:256904/1‑144 (MQ=255)
           atcgttCGATTCGGCTGGTGCGGTGAATGGGGCTTGTACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGGTGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGtat                                                                                                                                        <  2:545814/144‑1 (MQ=255)
                  ggttCTGCTGGTGCTGTTAGTGGTGGTTGCACTTTTCAATGATTTCTCTCGGTTAGGAGAGTTAGTTAGCAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCAGGGCGGCGCTGCTGTTGAGTAGTGCCACCGTATACGGTGc                                                                                                                                 <  2:369402/147‑1 (MQ=255)
                  gATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGc                                                                                                                                 >  1:239875/1‑149 (MQ=255)
                    ttCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGGTAGTTAGGAAGAACGCATAATAACGATGGCGAGGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTg                                                                                                                               <  1:67293/149‑1 (MQ=255)
                       tgctgGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGACCAAGACCCAGCAAGCGGCGCTGCTGTTTAGCAGCGGCACCGAATACGGTGCTGAAg                                                                                                                            >  1:529904/1‑149 (MQ=255)
                       tgctgGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAg                                                                                                                            >  2:639778/1‑149 (MQ=255)
                                     aTGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTGTACGGTGCTGCAGGGTTCGTAGTGaaa                                                                                                              >  2:533967/1‑149 (MQ=255)
                                     aTGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGaaa                                                                                                              <  2:584188/149‑1 (MQ=255)
                                     aTGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGaaa                                                                                                              <  2:287602/149‑1 (MQ=255)
                                            ttGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATATCGATGGCGATGAAAAAGTTGCTCATAGCATCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTc                                                                                                       <  2:634420/149‑1 (MQ=255)
                                                aCTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCAttt                                                                                                   >  1:146176/1‑149 (MQ=255)
                                                    ttCAATGATTTCTGTCGGTTATGAGAGTTAGTTAGGAAGAACGCGTAATGACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGaa                                                                                               <  2:699246/149‑1 (MQ=255)
                                                    ttCAATGATTTCTCTCAGTTATGATAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGaa                                                                                               <  1:232749/149‑1 (MQ=255)
                                                       aaTGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAACAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGc                                                                                            >  2:722761/1‑149 (MQ=255)
                                                       aaTGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAACAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGc                                                                                            >  2:722765/1‑149 (MQ=255)
                                                       aaTGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGACGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGc                                                                                            >  1:476375/1‑149 (MQ=255)
                                                        aTGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGcc                                                                                           >  1:471596/1‑149 (MQ=255)
                                                            tttCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGAATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCa                                                                                       <  1:210882/149‑1 (MQ=255)
                                                                tctcGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCgt                                                                                   >  2:481660/1‑149 (MQ=255)
                                                                tctcGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCgt                                                                                   >  1:30843/1‑149 (MQ=255)
                                                                          gagaGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATATCGAAGGCCTTCAGCGTGTCGCCGTTg                                                                         >  1:520002/1‑149 (MQ=255)
                                                                          gagaGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGGTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTg                                                                         >  1:7148/1‑149 (MQ=255)
                                                                          cagagTTAGTTAGGAAGAACGCATAATCATGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTg                                                                         <  1:359031/148‑1 (MQ=255)
                                                                                tagttagGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCgg                                                                   <  2:146176/149‑1 (MQ=255)
                                                                                   ttagGAAGAACGCATAATAACGATGGCTATTGAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGccc                                                                <  2:226134/149‑1 (MQ=255)
                                                                                       gaagaaCGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCcgtcct                                                            >  1:216551/1‑149 (MQ=255)
                                                                                          gaaAGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAg                                                         >  2:370466/1‑149 (MQ=255)
                                                                                            aCGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAATAACTTCAGCGTGTCGCCGTTGGTGCGGCCATCCTCAgac                                                       >  2:137965/1‑147 (MQ=255)
                                                                                            aCGCACAACAACAAGCGCGATGAAAAAGTTGCTCATAGCGCCGCTGCTGTTTGGCAGCGCCACCGTTGACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATGTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTa                                                       <  2:259555/149‑1 (MQ=255)
                                                                                                 taataaCGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTCCAGAGTGTCGCCGTTGCTGTGGCCCTCCTCCTTATGCCg                                                  <  2:612945/149‑1 (MQ=255)
                                                                                                     aaCGATGGCGATGAAAAGGTTGCTCATAGCGTCGGCGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGATGGTGCGGCGCTCCTCAGTATGCCGGTgc                                              <  2:447221/149‑1 (MQ=255)
                                                                                                           ggCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTTCGGCCCTCCTCAGTATGCCGGTGCGCACAg                                        >  1:27089/1‑149 (MQ=255)
                                                                                                           ggCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAACAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAg                                        <  1:722765/149‑1 (MQ=255)
                                                                                                           ggCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAACAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAg                                        <  1:722761/149‑1 (MQ=255)
                                                                                                            gCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGACGGTGCGCACAgg                                       >  2:438713/1‑149 (MQ=255)
                                                                                                              gATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCg                                     >  1:340049/1‑149 (MQ=255)
                                                                                                                   aaaaGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACg                                <  1:109262/149‑1 (MQ=255)
                                                                                                                   aaaaGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACg                                >  1:359304/1‑149 (MQ=255)
                                                                                                                     atgttgttCGTAGCGGCGGTGCGGTTTAGCAGCGGCACCGTATACGGTGCTGAAGGGTTCGGAGCGAAAGATATTCATCTCGAAGCCCTTCCGCGTGTCGCCGTTGGTCAGGCCCTCCTCAGCATGCCGGTGCGCACAGGCGACACggc                              <  2:473192/147‑2 (MQ=255)
                                                                                                                       gTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGGCACGGTTa                            >  2:481663/1‑149 (MQ=255)
                                                                                                                        ttGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTaa                           >  2:291013/1‑149 (MQ=255)
                                                                                                                           cTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAatga                        <  2:442109/149‑1 (MQ=255)
                                                                                                                                       ctgctgTTTAGCAGCGCCTCCGTTGACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAg            <  1:495572/149‑1 (MQ=255)
                                                                                                                                        tgctgTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGCATACCATTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGt           >  1:681684/1‑149 (MQ=255)
                                                                                                                                         gctgTCTAGCAGCGCTACCGTATACGGTGTTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTa          <  1:308881/149‑1 (MQ=255)
                                                                                                                                             tttAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTTCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATAc      <  2:236126/149‑1 (MQ=255)
                                                                                                                                                 gcagcGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCAtt  >  1:51843/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATT  >  NC_000913_3_hsa_tpiA/197823‑198116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑