Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 4,111,662 G→A 100% L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,111,6620GA100.0% 140.1 / ‑6.7 53L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/22);  minor base C (1/3);  total (28/25)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGC  >  NC_000913_3_hsa_tpiA/4111519‑4111802
                                                                                                                                               |                                                                                                                                            
aGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCt                                                                                                                                         >  1:130512/1‑149 (MQ=255)
  gAAGATGGCCGGCGCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCCCAAAGGCTTTGCTTTTTGTCATTTCTTCCAACCTTCATGCGATTCAGAGCGCGCCAGCTTAAGCGGTTGAACAGCGAAGGTCAACCCCttt                                                                                                                                       <  2:143777/149‑1 (MQ=255)
     gATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCTTCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATCCAGATAGCGCCAGCTTAATCGGTTCACAAGCGAAAGTTACCCCCTTTTCg                                                                                                                                    >  2:120036/1‑149 (MQ=255)
       cggCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACTGCGAAGGTCAACCCCTTTCCGcc                                                                                                                                  <  2:690475/148‑1 (MQ=255)
        ggCCGCCCCGCAGTGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCTGCACAAACGCTTTACTTTGTGTCATTTATTCAAACCTACAACCGATTCAGACAGCGCGTCCTTAATCGGTTCAACATCGTTGGTCAACCCCTTGTCGCCg                                                                                                                                 <  2:234399/149‑1 (MQ=255)
          ccgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGtt                                                                                                                               >  1:91833/1‑149 (MQ=255)
            gccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCACCGTTGt                                                                                                                             >  2:244830/1‑149 (MQ=255)
              cccGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTGCTCATTGATTCACCCTTTCAAGCTATTCAGATAGCGCCAACTTAATCGGTTCAACAGCGAATGTCAACCCCTTTTCACCGTTGTcc                                                                                                                           >  1:717384/1‑149 (MQ=255)
                         gcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATa                                                                                                                <  2:472369/149‑1 (MQ=255)
                           agGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAAc                                                                                                              <  1:19314/149‑1 (MQ=255)
                             gTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCACCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                                                                                                            >  1:241351/1‑149 (MQ=255)
                               cTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCATTTTCGCCGTTGTCCGCGCCAACATAACGCAg                                                                                                          >  1:597806/1‑149 (MQ=255)
                                tgtaAAACAGTATAGAGTTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                         <  1:653782/149‑1 (MQ=255)
                                          tataGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTg                                                                                               <  1:545363/149‑1 (MQ=255)
                                          tataGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAACAAGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACACCATAACGCGGTGCACTTTCTg                                                                                               >  1:534237/1‑149 (MQ=255)
                                                 atgcaacGGTACAAAGGCTTTGCTTTTTAACATTTATTCAATCCTTCAAGCGATTCAGACAGCGCCCGCTTAATCGGGTCAACACCGAAGGTCAACCCCTTCGCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgt                                                                                        <  2:556444/143‑1 (MQ=255)
                                                            caAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgtt                                                                             <  1:496945/149‑1 (MQ=255)
                                                             aaaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGCTGTAATACCgttt                                                                            >  2:312644/1‑149 (MQ=255)
                                                              aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGACGTATTTCTCGACAACAT‑ACGCAGTGCACCTTCTGTCTCGGTGTAATAACgtttgt                                                                          >  1:684316/1‑149 (MQ=255)
                                                               aGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgtttgt                                                                          <  1:85023/149‑1 (MQ=255)
                                                                 gctttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgtttgttt                                                                        <  1:722455/149‑1 (MQ=255)
                                                                   tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGttttt                                                                      <  1:700374/149‑1 (MQ=255)
                                                                    ttcctttTGTTCATTTCTTCATACCTTTAATCCATGCACCTCCCGCCCGCTTACCCGGTTTAACAGCGTAGGTCTACCCCCCTCCCTCGTTGTCTGCGAAAACATACTCCACTGCACCTTCTCTCTCGGTGTAATAACGTTTGTTTTTc                                                                     <  2:57556/146‑1 (MQ=255)
                                                                     tgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcc                                                                    <  1:683808/149‑1 (MQ=255)
                                                                       ctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCTCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcccc                                                                  <  1:26884/149‑1 (MQ=255)
                                                                        tttttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGAAAACATAACGCAGTTCACCTTCTGTCTCGGTCGTCTATCGTTTGTTTTTTCccg                                                                 >  2:357312/1‑149 (MQ=255)
                                                                              tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTta                                                           <  1:252027/149‑1 (MQ=255)
                                                                                aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                         >  1:423620/1‑149 (MQ=255)
                                                                                aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                         >  1:28598/1‑149 (MQ=255)
                                                                                     ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATCACGTTTGTTTTTCCCCGCCGTTAGTAGGGa                                                    >  1:198167/1‑149 (MQ=255)
                                                                                     ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTGGTCCGCGACAACATAACGCAGGGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCgt                                                    >  2:349389/1‑148 (MQ=255)
                                                                                        aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTg                                                 >  1:126763/1‑149 (MQ=255)
                                                                                           ccTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaac                                                                                                                   <  2:154205/80‑1 (MQ=255)
                                                                                           ccTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaac                                                                                                                   >  1:154205/1‑80 (MQ=255)
                                                                                                aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTGGCGACTGCAGgtttt                                         >  1:427113/1‑149 (MQ=255)
                                                                                                  ccGATTCAGAGAGCGCCAGCTTAACCGGTTCACCAGCTAAGGTCTCCCACAAGTCGCGGGTGTCCGCGTCAACATAACGCAGTGCACCGTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                       <  1:683874/148‑1 (MQ=255)
                                                                                                      ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc                                   >  1:145950/1‑149 (MQ=255)
                                                                                                         aGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCACCCCCTTTCTGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCGTTATCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCttt                                <  2:145950/149‑1 (MQ=255)
                                                                                                           aTAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                              <  1:401095/149‑1 (MQ=255)
                                                                                                             aGCGCCAGCTTAATCGGTCAAACAGCGAAGGTCACCCCCTTTTCGCCGTAGGCCGCGACAACATAACGCAGTGCACCTTCTGGCACGGCGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTgtgt                            <  1:244830/149‑1 (MQ=255)
                                                                                                                   tgCTAAAACGGTTCAACTTCAAAGGTAAACCCGTTTTGCCTGTTGTCATCGACAACATACCTCAGTGCACCTTGTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                      <  2:405742/148‑1 (MQ=255)
                                                                                                                     cTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                    >  1:108001/1‑149 (MQ=255)
                                                                                                                      ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACg                                                                                                             <  2:180719/59‑1 (MQ=255)
                                                                                                                      ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACg                                                                                                             >  1:180719/1‑59 (MQ=255)
                                                                                                                      ttAATAGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                   >  2:555663/1‑149 (MQ=255)
                                                                                                                          tCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCACCGTTAGTAGCGAATGCAGTTTTCTCTGGCTTTGTGTTTTGGTCATTAAt               >  1:460501/1‑149 (MQ=255)
                                                                                                                          tCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTGTTTCACCGACGTTAGTAGCGACTGCAGCTATCTCTCGCTTTGTGTTTTGGTCCTTCAt               >  2:115603/1‑149 (MQ=255)
                                                                                                                              ttCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATgggg           >  2:343278/1‑149 (MQ=255)
                                                                                                                               tCAACCGCGAAGCTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt          <  1:67433/149‑1 (MQ=255)
                                                                                                                                cAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTCATGGGGTg         >  2:531106/1‑149 (MQ=255)
                                                                                                                                cAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTAATTAATGGGGTg         >  1:196878/1‑149 (MQ=255)
                                                                                                                                   cAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgt      <  1:404664/149‑1 (MQ=255)
                                                                                                                                       gAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGAGGGGAggtggt  >  1:494962/1‑144 (MQ=255)
                                                                                                                                               |                                                                                                                                            
AGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGC  >  NC_000913_3_hsa_tpiA/4111519‑4111802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑