Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 4,513,088 C→G 9.2% A102P (GCG→CCG)  fecC ← ferric citrate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,513,0880CG 9.2% 112.7 / 2.3 45A102P (GCG→CCG) fecCferric citrate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (16/23);  new base G (2/3);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCT  >  NC_000913_3_hsa_tpiA/4512943‑4513236
                                                                                                                                                 |                                                                                                                                                    
tttCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGTCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAgcgccgc                                                                                                                                                   <  1:558923/149‑1 (MQ=255)
      tCATGTGTATGACGAAATCCGCCTCCTGCCGTCATGACCAGCAGCCCGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCCTCGGACTCAGCGCGCTGGTCAGCGCCATCAACAGCGCCGCGCCGCt                                                                                                                                             >  2:525255/1‑149 (MQ=255)
        aTGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGCTGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCCCACCCCTGt                                                                                                                                           >  2:215554/1‑149 (MQ=255)
         tGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGtt                                                                                                                                          >  1:91964/1‑149 (MQ=255)
         tGGGTATGACGAAATCCGCCTACTGCGGCCTGAACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCATCGCCGCGCCGCTGtt                                                                                                                                          >  1:174534/1‑149 (MQ=255)
             tATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGACAGCGACGCGCCGCGGTTAATg                                                                                                                                      >  1:457576/1‑149 (MQ=255)
              aTGACGAAATCCGCCTCCGGCGGGCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGc                                                                                                                                     <  1:330408/149‑1 (MQ=255)
               tGACGAACTCCGCCTCCTGCAGTCATGCCCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGACTGCCAGAGAATAGCCGGCACTCGGCGTCGGACTCACCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCCCTCTGACTGcc                                                                                                                                    >  2:709425/1‑149 (MQ=255)
                   gAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCCTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCACCTGTTAATGCCCAGc                                                                                                                                >  2:377957/1‑149 (MQ=255)
                               ctGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGGGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGCGCAGTGAAGGagag                                                                                                                    >  1:155999/1‑149 (MQ=255)
                                     tCATGACCAGCAGCCAGCTCACGCCGCCCCGGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGCCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCCCCGCGGTTAATGCCGAGCCGTGACGGAGCGGCCact                                                                                                              >  2:584843/1‑147 (MQ=255)
                                     tCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCCGCGGCGGACTCAGCGCGCTGGTACGCGCCATAGGACGCGCCGCGCCGCTGTTACTGCGGACCAGTGAAGAGGAGGCCAtt                                                                                                              >  2:38736/1‑149 (MQ=255)
                                        tGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTggg                                                                                                           <  1:61604/149‑1 (MQ=255)
                                            cagcagCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTtgt                                                                                                       <  2:300927/149‑1 (MQ=255)
                                             agcagcCAGCTCACCCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTtgtg                                                                                                      <  1:523863/149‑1 (MQ=255)
                                              gcagcCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTgg                                                                                                     <  1:559067/149‑1 (MQ=255)
                                                    agcTCACGCGGCCCCCGCACGACGCAACTCATGACAGTGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTACGCGCCATAGCCAGCGCCGCGCCTCTGTTAATGCCGAGCAGTGAACTAGAGGCCCTTCTGTTGTGGGTCatg                                                                                               <  2:515089/149‑3 (MQ=255)
                                                        cACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGttt                                                                                           <  2:442300/149‑1 (MQ=255)
                                                         aCGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCCTTGGGTTGTGGGTCAGGGTTTg                                                                                          >  1:93340/1‑149 (MQ=255)
                                                                ccccGCATGCCTCGATTCATGACATAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCAATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCagcagc                                                                                   <  1:671059/149‑1 (MQ=255)
                                                                ccccGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCagcagc                                                                                   <  2:91964/149‑1 (MQ=255)
                                                                ccccGAATGCCGGGATGAATGACAGAGAATAGCCTGGAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCagcagc                                                                                   <  1:251161/149‑1 (MQ=255)
                                                                  ccGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAACGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGt                                                                                 <  1:387201/149‑1 (MQ=255)
                                                                    gCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGc                                                                               <  1:13535/149‑1 (MQ=255)
                                                                             gATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAgcg                                                                      >  1:455605/1‑149 (MQ=255)
                                                                                gaatgaCAGGGAATAGGCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAATCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCa                                                                   <  2:92732/149‑1 (MQ=255)
                                                                                 aatgaAAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAg                                                                  <  1:496180/149‑1 (MQ=255)
                                                                                 aacgacagacaATAGCCCGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCGGGGCCGCTGTTAATGCCGAGCAGCGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAg                                                                  <  2:562393/146‑1 (MQ=255)
                                                                                        gagaATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTgcg                                                           <  2:39655/149‑1 (MQ=255)
                                                                                                     caTCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACgg                                              <  1:90903/148‑1 (MQ=255)
                                                                                                      aTCGGCGACGGACTCAGCGGGCTGGCAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAAAGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCGTGCGCCGATCAGAACGGc                                             <  2:713505/149‑1 (MQ=255)
                                                                                                        cGGCGTCGGACCCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTCATGCCGAGCGGTGAAGGGGAGGCCATTTGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGCTCGGAACGGCGa                                           <  1:28616/149‑1 (MQ=255)
                                                                                                                     aGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTgg                              <  1:570404/149‑1 (MQ=255)
                                                                                                                           cTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACg                        >  1:409949/1‑149 (MQ=255)
                                                                                                                             ggTAAGGGCCATAGCGAGCGCCGCGCTGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCTGTGTGAACGGCGACCAGGCTTCGTGGCAAACGaa                      <  2:703236/149‑1 (MQ=255)
                                                                                                                                 aGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGGGAAGGAGAGGCCATTGGGTTGTGGGTCAAGGCCTCCACGAGGATTCTTGTGAGCGCCATGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGCAGGtt                  >  2:442942/1‑149 (MQ=255)
                                                                                                                                  gcgcCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGttt                 <  1:552283/149‑1 (MQ=255)
                                                                                                                                    gcCATAGCCAGCGGCGCGCCGCTGCTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTg               <  2:68380/149‑1 (MQ=255)
                                                                                                                                    gcCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTg               >  1:675792/1‑149 (MQ=255)
                                                                                                                                        ttaacAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAg                     >  1:440591/5‑139 (MQ=255)
                                                                                                                                        ttaacAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAg                     <  2:80281/135‑1 (MQ=255)
                                                                                                                                        ttaacAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAg                     <  2:440591/135‑1 (MQ=255)
                                                                                                                                        ttaacAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAg                     >  1:80281/5‑139 (MQ=255)
                                                                                                                                          gCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAg         <  1:704191/149‑1 (MQ=255)
                                                                                                                                           ccAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATCGGGTTGTGGGTCACGGTTTGCCGCAGCGTGCCCGCGAGCGCGAGGCTTCTGCCCAACAGAACTGCGACCACGCTTCGTTGCAACCGGAGGTTTTGCACCAgc        >  2:151943/1‑149 (MQ=255)
                                                                                                                                            cAGCGCCGCGCCGCTGTTAATGCCGAGCAGTAACACCAAGTCCATTGGGTTGTGGCTCTGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCCGGCTTCGTGGCAAACGACGGTTCTGCACCaccc       >  2:702738/1‑146 (MQ=255)
                                                                                                                                                gccgcgccgcTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGGAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAGCGGCGACCAGGCTTCGTGGCAAACGAGGGTTTTGCGCCAGCGCTTc   >  2:192893/1‑149 (MQ=255)
                                                                                                                                                 ccgcgccgcTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCt  <  1:400161/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCT  >  NC_000913_3_hsa_tpiA/4512943‑4513236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑