| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_hsa_tpiA | 282,138 | A→G | 10.9% | intergenic (‑155/‑140) | yagA ← / → yagE | CP4‑6 prophage; putative DNA‑binding transcriptional regulator/2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_tpiA | 282,138 | 0 | A | G | 10.9% | 75.8 / 3.8 | 38 | intergenic (‑155/‑140) | yagA/yagE | CP4‑6 prophage; putative DNA‑binding transcriptional regulator/2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage |
| Reads supporting (aligned to +/- strand): ref base A (19/14); new base G (2/2); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01 | |||||||||||
TTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCC > NC_000913_3_hsa_tpiA/281994‑282282 | ttATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTAAGGGGCAattt > 1:556727/1‑147 (MQ=255)ttATGACTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAatat < 1:125359/149‑1 (MQ=255) ctgcgTTGTACTTGTCTACCATGTCCCCGAACAAGTGTTGACTATGTCCCCGGACCGTACACCCCAAAGGGGAGATGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCTCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATc < 2:496565/145‑1 (MQ=255) cTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATc < 1:317587/149‑1 (MQ=255) ttAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTAACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCCCACCCCTTTGGGGAGGGGGCCGGGGGGAGGGGCAAAATGTGAAcc > 1:609299/1‑149 (MQ=255) gtgtCTACCATGTCCCCGAACAAGGGTTCACTATGTCACCGGAACGTACACCCCAAAGGGGAGAGGAGACTGCACCGAGCCATCTTTTCCCCCTGGCCACTTCGAGGGGGGGGCTGGGCGGAGGTTCAGTATGCGACCCAATTTATAtt > 2:570643/1‑149 (MQ=255) tgtCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCGATATGTGATCCAGCTTAAAttt > 1:393428/1‑149 (MQ=255) tgtCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCGATATGTGATCCAGCTTAAAttt > 1:34012/1‑149 (MQ=255) tCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCAAATCCACCGCTTCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTcc > 1:167980/1‑149 (MQ=255) ccATATCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAAGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGAGCCGGGGTGAGGGCCAATATGTGCTCCGGCTTCAATTGccggta > 2:75772/1‑145 (MQ=255) gTCCCCGAACAAGTGTTCACTATGGCCCCGGACCGTACAGCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTcc < 2:594195/149‑1 (MQ=255) ccGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGCGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGAACTCCctct > 1:695402/1‑149 (MQ=255) ccGAACAAGTGTTCACTATGTCCCCGGACCGTACACACTAATTTTGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCctct > 1:720460/1‑149 (MQ=255) tgtTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCg > 1:297855/1‑149 (MQ=255) gtTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGAGCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGa > 1:370804/1‑149 (MQ=255) aCTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGCCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTa > 2:718096/1‑149 (MQ=255) cccGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGACGGCCGGGGTGAAGTGCAATATGCAATCCAGCTTAACTTTCCCGCACTCCCTCTTCCCTTCCGCTTTACCACTCCt > 2:40075/1‑149 (MQ=255) ccGGACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCTCTCGCCCCTTCGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCt < 1:300749/149‑1 (MQ=255) tACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCg > 2:607957/1‑149 (MQ=255) ccAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAg > 1:175595/1‑149 (MQ=255) aaaGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTa < 1:502901/149‑1 (MQ=255) aGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGGGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCGTCCGATTTACCTCTCCTTGTTCTCCGTCATAGtcgg > 1:186954/1‑146 (MQ=255) ggAGAGGGGACTGCACCGATCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCTGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATc < 1:80556/149‑1 (MQ=255) ggAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCGATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATc < 2:34012/149‑1 (MQ=255) ggAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCGATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATc < 2:393428/149‑1 (MQ=255) gagGGGACTGCACCGCGCGATCTTTTCCCCCTTGCCCTTTTGGGGAGAGGGCCGGGGTGATGGGGAATATGTGATTCAGTTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGtt < 2:222484/149‑1 (MQ=255) gggACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTtaaa < 2:172363/149‑1 (MQ=255) ccATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGAGATCCAACTTAAATTTCCCCCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGt > 1:19379/1‑149 (MQ=255) aTCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTTCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTGATAGGATGATCGTTTAATAAACGAACGCTCTTc > 2:598722/1‑149 (MQ=255) ttttCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCtat > 1:253579/1‑149 (MQ=255) cccccTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATg < 1:617607/149‑1 (MQ=255) ccccTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTTTATTGt > 1:400127/1‑149 (MQ=255) cccTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTa < 1:534792/149‑1 (MQ=255) ccTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACaaaa < 1:452187/149‑1 (MQ=255) agGGCCGGGCTGAGGGGCCATACGTGATCCAGCTTAAATCTCCCGCCCTCCCTCTTCCATTCCGATTTAACTCTCCTTGCTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCaa < 2:597833/149‑1 (MQ=255) gCCGGGTTGAGGCGCAATATGTGATTCATTTTATATTTCCCGCACTCCCTCTTACCTTCCGTTTTACCGTTCCTTTTTCTGCGTCATAGTATGAGCGTTAAATATACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGga < 2:281449/149‑1 (MQ=255) aaGGGGCAATATGGGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATCACGTAGAACAAAATGATTCAGCAAGGAGATCTCAt < 2:407360/148‑1 (MQ=255) gggCAATATGTCATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCCTCATAGTATGATCGTTAAATAAACGAACCCTGTTCCATAATGTAGAACAAAATGATTCATCAAGCAGACTCCATGcc > 2:701339/1‑149 (MQ=255) | TTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTCGCCCCTTTGGGGAGAGGGCCGGGGTGAGGGGCAATATGTGATCCAGCTTAAATTTCCCGCACTCCCTCTTCCCTTCCGATTTACCTCTCCTTGTTCTGCGTCATAGTATGATCGTTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCC > NC_000913_3_hsa_tpiA/281994‑282282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |