Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 430,866 G→A 10.2% A88T (GCA→ACA)  yajD → HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA430,8660GA10.2% 83.1 / 2.0 39A88T (GCA→ACA) yajDHNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base G (19/14);  new base A (2/2);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

GTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGC  >  NC_000913_3_hsa_tpiA/430723‑431007
                                                                                                                                               |                                                                                                                                             
gTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAggg                                                                                                                                          >  1:95041/1‑149 (MQ=255)
      ttACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGCTGGCAATAACTGGGAATTGTTGTGTCTGTATTGCCACGATCATGAGCATTCGAAATATACCAAAGCGGATCAGAATAGTACGACCGTGATCTCAGGGGAAGAt                                                                                                                                    >  1:727038/1‑149 (MQ=255)
         ccGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATgcg                                                                                                                                 >  2:591531/1‑149 (MQ=255)
               accacATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGaaa                                                                                                                           >  1:8152/1‑149 (MQ=255)
                   caTTGATCACGACCGTACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATg                                                                                                                       >  2:566883/1‑149 (MQ=255)
                        aTCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGt                                                                                                                  <  1:256664/149‑1 (MQ=255)
                           aCGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGGAGATGCGCAGAAAGATGTCGGTaaa                                                                                                               >  1:693961/1‑149 (MQ=255)
                                  tACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGAAGAAAGATGTCGGTGAAGCGAAGt                                                                                                        >  2:99207/1‑149 (MQ=255)
                                      aaTATCCCGGAAGATGGCAGTAACGGGGAAGTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCAAATAAGTATGCGACGAGCGTTAACAAATGGTAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACaa                                                                                                    <  2:446055/149‑1 (MQ=255)
                                      aaTAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACaa                                                                                                    >  1:15835/1‑149 (MQ=255)
                                             cGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCACAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGaa                                                                                                          <  1:54398/136‑1 (MQ=255)
                                             cGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCACAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGaa                                                                                                          >  2:54398/1‑136 (MQ=255)
                                                   aTGGTAGTAACTGGCGATTGTTGGTGCTCTATTGCCAAGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGAc                                                                                       <  2:627369/149‑1 (MQ=255)
                                                     ggCAGTAAATGGGAATGGTTGTGACTCTATTGCCACGATCATGAGCATTCGAAATAGACCGGAGCTGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAATCCATTCGCTGACCt                                                                                     <  2:166184/149‑1 (MQ=255)
                                                             cTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCga                                                                             <  1:566883/149‑1 (MQ=255)
                                                               gggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCgatg                                                                           >  1:566258/1‑149 (MQ=255)
                                                                tgAATTGTTGTGGTTCTATTACCACGATCATGAGAATCAGAAATATACCGAAGCGGGTCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCgatga                                                                          <  2:473225/148‑1 (MQ=255)
                                                                ggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCgatga                                                                          >  1:501212/1‑149 (MQ=255)
                                                                       ttgtGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACaa                                                                   <  1:27924/149‑1 (MQ=255)
                                                                         gtgtCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCTCAGAGGAAGATGCGGAGAAATATGTCGGCTAGTGGAAGTGCACCCCAATCGCTGACCTTACAGCGAcgaagacaacga                                                                 >  2:260861/1‑143 (MQ=255)
                                                                             ctccATTGCCACGATCATGAGGATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGGACAACCCATTCGCTGACCTGAAAGCGATGATGAACaagaagaa                                                             <  2:330698/149‑1 (MQ=255)
                                                                              tctATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACaagaagaag                                                            <  2:566258/149‑1 (MQ=255)
                                                                              tctATTGCCACAATCGTGAGCATTCGAAAGATACTGAAGTGGATCAGTATGGTACGACCGTTCTCGCAGGGGAAGATGCGCAGAAAGCTGTCGGTGAAGCGAAGTATAACCCATTCGCTGACCTGAAAGCGATGATGAACaagaagaag                                                            <  2:486505/149‑1 (MQ=255)
                                                                                  ttGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGAt                                                        <  1:254308/149‑1 (MQ=255)
                                                                                     ccACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTaa                                                     >  1:312430/1‑149 (MQ=255)
                                                                                       aCGAACATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGAGTaaaa                                                   <  2:124304/149‑1 (MQ=255)
                                                                                             aTGAGCATTCGAAATATACCGAAGCGGATCAGTAAGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTaaa                                             >  1:538061/1‑149 (MQ=255)
                                                                                              tGAGCATTCGAAATATACCGAAGCGGTTCAGTATGGTACAACCCTTTAAACAGGTGAAGATCTTTATATATATGTCGGTGAAGCGAAGAACAACCCATTCGCTGACCTGAAAGCGATGATGAACAGGAAGAAGTGATTAAAACGTaaaa                                            >  1:352410/1‑149 (MQ=255)
                                                                                                 gCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTg                                         >  2:134917/1‑149 (MQ=255)
                                                                                                       gAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATAGCAGGGGAAGATGCACAGAAAGATGTCGGTGAAGTGACGTACAACCCATTCGCTGTCCTGAAAGCGATGATGAAAAGGAAGAAGTCATAAAAACGTAAAATTGCATGAt                                   >  2:35357/1‑149 (MQ=255)
                                                                                                        aaaTATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTGATg                                  >  2:409856/1‑149 (MQ=255)
                                                                                                         aaTAAACCTAAGCGGATCAGTATGGTACGACCGTGAACGCAGGGGAAGAGGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTGATgc                                 <  1:234691/149‑1 (MQ=255)
                                                                                                           tataCCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTGATgcgc                               <  2:312430/149‑1 (MQ=255)
                                                                                                                cGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTCAGGCGCTACGc                          >  1:648677/1‑149 (MQ=255)
                                                                                                                  aaGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAAc                                                  >  1:108759/1‑123 (MQ=255)
                                                                                                                  aaGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAAc                                                  <  2:108759/123‑1 (MQ=255)
                                                                                                                       gATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTCAAAGCGATGATGAACAACGAGAAGTGATTAAAACACAAAATTGCCTGATGCCCTACGCTTATCAg                   >  2:78861/1‑149 (MQ=255)
                                                                                                                                  tACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGttct        >  1:306695/1‑147 (MQ=255)
                                                                                                                                        cGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGCAGTACAACCCATGCGCTGACCTGAAAGCAATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTGATGCGCTACGCATATCACGCCTACCTCCTTTCAGc  >  2:300870/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                             
GTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAGTACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGATTAAAACGTAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGC  >  NC_000913_3_hsa_tpiA/430723‑431007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑