Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 482,230 G→A 100% P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA482,2300GA100.0% 148.7 / ‑6.7 51P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/24);  minor base C (1/0);  total (27/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGG  >  NC_000913_3_hsa_tpiA/482086‑482371
                                                                                                                                                |                                                                                                                                             
acatAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGt                                                                                                                                           <  2:558649/149‑1 (MQ=255)
acatAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGt                                                                                                                                           <  2:535535/149‑1 (MQ=255)
  atAAACTTTCTTCACACGACCGCGGTCGCTAAAGTCGTTCACATAGCTGCCCCCCCATGCAGCGCCCAGAGTGGTGTTTATGTCGTTGATATAAACACCCAGCGACTGCGCTTGTTCCTGTTCGATATGAACTGTAAACTGCAGGGtgt                                                                                                                                         >  2:492367/1‑147 (MQ=255)
    aaaCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCt                                                                                                                                       >  1:219484/1‑149 (MQ=255)
     aaCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCtt                                                                                                                                      >  2:492922/1‑149 (MQ=255)
      aCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                     >  1:446204/1‑149 (MQ=255)
        tttCGTCGCACTAGCTCGCTCTATAAAGTCGTTCACATGCCTTCCGCCCCATCTAGCGCCCATAGTCGTGTTAATGTCGTTGATATAAACACCCATCTCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCATGGTATCTTCCa                                                                                                                                   <  2:386059/149‑1 (MQ=255)
            ttcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATGTTAAACTGCAGGGTATCTTccgtacc                                                                                                                               >  1:244433/1‑144 (MQ=255)
                cacGTCCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                           <  2:682752/149‑1 (MQ=255)
                cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                           <  1:400469/149‑1 (MQ=255)
                 acGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTg                                                                                                                          >  1:154556/1‑149 (MQ=255)
                 acGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTg                                                                                                                          >  2:618257/1‑149 (MQ=255)
                 acGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTg                                                                                                                          >  2:531484/1‑149 (MQ=255)
                      cgcgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGt                                                                                                                     >  1:600740/1‑149 (MQ=255)
                        cgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATACATCTTAACCTGCAGGGTAGCTTCCAGACCGTTTGGACGTAc                                                                                                                   >  1:133180/1‑149 (MQ=255)
                           tCGATAAAGTAGTTCACACCGCTGCCGCCCCATGCAGCGCCAAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCt                                                                                                                <  2:58997/149‑1 (MQ=255)
                             gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGCGTGGTGGTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCCATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAACCTgg                                                                                                              >  2:303236/1‑149 (MQ=255)
                                    tCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACat                                                                                                       >  1:18705/1‑149 (MQ=255)
                                        tCACATAGCTGCCGCCCCGTGCAGCACCCAGAGTGGTGTTAATGTCGGTGATAGAAACACCCAGCGGCTGCGGTTTTTCCGGGTCGGTATCAATCTTAAACTGCCGGGCATCTTCAAGACCGATTGGACGTACGCTGGTCAACaggtca                                                                                                   >  2:368292/1‑144 (MQ=255)
                                          acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                 <  2:219484/149‑1 (MQ=255)
                                            aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTATTAATGTCGTTAATAGACACACCCAGCGCCGGCGGTTTTGCCTGGTCTGTATCTATCTTCAGCTTCAGGTTATCCTCCAGACCGTTTGTACGTACGCTGGCACAAATATCAGGGt                                                                                               >  2:362849/1‑149 (MQ=255)
                                                   ccgccCCCTGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgc                                                                                        <  2:210487/149‑1 (MQ=255)
                                                    cgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                     >  1:541170/1‑103 (MQ=255)
                                                    cgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTc                                                                                                                                     <  2:541170/103‑1 (MQ=255)
                                                     gccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgctg                                                                                      >  1:727366/1‑149 (MQ=255)
                                                      ccccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgctgc                                                                                     >  1:339427/1‑149 (MQ=255)
                                                            gCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgc                                                                               <  1:670595/149‑1 (MQ=255)
                                                              aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                             <  2:523050/149‑1 (MQ=255)
                                                                                   gTCCTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGAAGTATGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAg                                                        >  2:671285/1‑149 (MQ=255)
                                                                                    tCGTTGATAGACACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCATTTGAACGTACGCTGGTCAACAAATCAGGGTGCTTCGCTGCTTCTGCAAGCACCTGGTTACGCCCGTGAgc                                                       >  2:207483/1‑148 (MQ=255)
                                                                                    tCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCCCTGCTTCTGCAACCAACTGGTTACGCGCCTGAGt                                                       >  2:28045/1‑149 (MQ=255)
                                                                                      gTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                     <  1:60490/149‑1 (MQ=255)
                                                                                         gATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGGAACTGGTTACGCGCCTGAGTCAGtt                                                  >  1:719197/1‑149 (MQ=255)
                                                                                           tAGAAACAGCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGGTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt                                                <  2:447237/149‑1 (MQ=255)
                                                                                             gAAACACCCAGCGCCTGCGCTTTTTCCTGCTCCTTATAAATCTTAAACTGCAGGCTATCTTCCAGACCGTTTGGACGTACGCTGCTCAACATATCAGGGTGCTTCGCTGCTCCTTCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                              <  1:160397/149‑1 (MQ=255)
                                                                                               aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                            <  1:281032/149‑1 (MQ=255)
                                                                                                  acCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAc                                         >  1:202091/1‑149 (MQ=255)
                                                                                                      aGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCAGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGCGTCAGTTTTTCGTGAcgacg                                     >  2:595821/1‑145 (MQ=255)
                                                                                                       gcgcCTGCGCTTTTGCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAgg                                    <  2:484859/149‑1 (MQ=255)
                                                                                                               tgtttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg                            <  2:545669/147‑1 (MQ=255)
                                                                                                                     tcatGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                      <  1:7999/146‑1 (MQ=255)
                                                                                                                      ccTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc                     <  1:597722/149‑1 (MQ=255)
                                                                                                                       cTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCATCTGCTAATGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCa                    >  1:712187/1‑149 (MQ=255)
                                                                                                                          gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCCGCCTGGTCAATCAGCt                 >  1:686614/1‑149 (MQ=255)
                                                                                                                          gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                 <  1:531484/149‑1 (MQ=255)
                                                                                                                          gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                 >  1:396882/1‑149 (MQ=255)
                                                                                                                              atatCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa             <  1:443808/149‑1 (MQ=255)
                                                                                                                               tatCAATCTTAAACTGCAGGGTATTTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCGTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaag            <  1:607922/149‑1 (MQ=255)
                                                                                                                                atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt           <  1:609673/149‑1 (MQ=255)
                                                                                                                                        tAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg   <  1:374558/149‑1 (MQ=255)
                                                                                                                                         gaaCTACAGGGTATCTTCCAGACCGTTTGGAGGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTGCTCTGCTGAGTCAGTTTTTAGTGACCAAGGCCAGCCTGCTCAATCAGCTCAAAGTCACAGCCgg  >  2:270813/2‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
ACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGG  >  NC_000913_3_hsa_tpiA/482086‑482371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑