New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 4542672	44 (0.740)	7 (0.130)	6/262	7.0	14.7%	intergenic (+57/‑425)	fimE/fimA	tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
	?	NC_000913_3_hsa_tpiA	= 4542968	40 (0.720)					intergenic (+353/‑129)	fimE/fimA	tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTG  >  NC_000913_3_hsa_tpiA/4542820‑4543114                                                                                                                                                     |                                                                                                                                                   ctctggtgCTCGACGCATCTTCCTCATTCTTCTCTCCAAAGACCACCTCATGCAATATAAACAGCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACGGAAAAAGTTTGTCCTCGATGCTTTCCTCTATGAGTCaaaa                                                                                                                                                    <  1:171872/142‑1 (MQ=255)   cTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATg                                                                                                                                                  >  1:255158/1‑149 (MQ=255)     gtgGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGc                                                                                                                                                <  2:244065/149‑1 (MQ=255)                 aTCTTCCTCATTCTTCTCTCCAAAACCCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTc                                                                                                                                    <  1:1016786/149‑1 (MQ=255)                  tCTTCCTCATTCTTCTCTCCAAAATCCTCCTCATGAAATATAAACATCAAAACAAAAAGATAACAATAGAATATTAAGCCAACGAATAATCTGAAAAAGTTTGTCCGCGTTGCTTTCCTCTTTGAGTAAAAATGGACTCAAATGGTTCa                                                                                                                                   <  2:293809/149‑1 (MQ=255)                  tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCa                                                                                                                                   >  2:614881/1‑149 (MQ=255)                    ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATc                                                                                                                                 >  1:66872/1‑149 (MQ=255)                      ccTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCtt                                                                                                                               <  2:250789/149‑1 (MQ=255)                                 tctcCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGaaaa                                                                                                                    >  2:884122/1‑149 (MQ=255)                                     cAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAAt                                                                                                                                         >  2:937217/1‑124 (MQ=255)                                     cAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAAt                                                                                                                                         <  1:937217/124‑1 (MQ=255)                                      aaaaaCCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATACTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACtgtg                                                                                                               >  1:682629/1‑149 (MQ=255)                                           ccaccTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGCAAACTGTGCAgtg                                                                                                          >  1:413627/1‑149 (MQ=255)                                           ccaccTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAgtg                                                                                                          <  2:255158/149‑1 (MQ=255)                                             accTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGtt                                                                                                        >  2:413692/1‑149 (MQ=255)                                              ccTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTg                                                                                                       >  2:87480/1‑149 (MQ=255)                                                   tGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGt                                                                                                  <  2:712602/149‑1 (MQ=255)                                                    gCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTc                                                                                                 >  2:509490/1‑149 (MQ=255)                                                      aaTATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCaa                                                                                               >  2:45878/1‑149 (MQ=255)                                                            aaCATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGt                                                                                         <  1:413692/149‑1 (MQ=255)                                                                  tataAATAAAGATAACAATAGAATATGAAGCCAACACAAATACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGCGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacga                                                                                   >  2:9648/1‑147 (MQ=255)                                                                  tataAAGAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaa                                                                                   >  2:493382/1‑149 (MQ=255)                                                                    taaataaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaa                                                                                 <  1:614881/149‑1 (MQ=255)                                                                       ataaaGATAACAATAGCATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCACATTTTGGCGGCAAACTGTGCAGTGTTGGCAGTCAAACTCGGTTacaaaacaa                                                                              >  2:217698/1‑149 (MQ=255)                                                                           aGATAACAATAGAATATTAAGCCAGCACATAAACTGAAAATGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATTGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACCCGTTTATAAAACAAAatg                                                                          <  2:872810/149‑1 (MQ=255)                                                                            gATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTACTCTATGAGTCAAAAAAGCCAAAGATGTTTCATTTTCTTCTTTAAATCTGTTCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAgtgt                                                                         >  1:198037/1‑149 (MQ=255)                                                                                    tAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAACCAAAGTGTACAGCACg                                                                 >  2:962939/1‑149 (MQ=255)                                                                                      gAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGAc                                                               >  2:331036/1‑149 (MQ=255)                                                                                           ttAAGCCAACAACTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGTTGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGccc                                                          <  1:148326/149‑1 (MQ=255)                                                                                                       aTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAg                                              <  1:726829/149‑1 (MQ=255)                                                                                                          aaCTGAAAAACTTTGTCCGCGATGCTTTCCTCTATGAGCCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAACACTGTGCAGTGTTGGCAGTCAAACTCGCTTACAAAAAAAAGGGTACAGAGCGAAGGCCCATGTCACATTAGaat                                           >  2:501255/1‑148 (MQ=255)                                                                                                            cTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATa                                         >  2:673228/1‑149 (MQ=255)                                                                                                               aaaaaGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGtt                                      <  1:884122/149‑1 (MQ=255)                                                                                                                  cgctttGTCCGCGATGGTTTCCTCTAGGAGTAAAACTGGCTCCCACTGTATCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGCCTGCCCATGTCCATTTAGAAATAGttttt                                   <  1:217698/146‑1 (MQ=255)                                                                                                                           cgggATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTGGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTgaaaggaa                          <  2:836954/146‑1 (MQ=255)                                                                                                                            gcgATGCTTTCCTCTATGAGGCAAAATGGCCCCAAATGTTTCATCTTGTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTgaaaggaaa                         >  1:414943/1‑149 (MQ=255)                                                                                                                                   tttCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCa                                                                                                    >  2:957105/1‑67 (MQ=255)                                                                                                                                   tttCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCa                                                                                                    <  1:957105/67‑1 (MQ=255)                                                                                                                                               gTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTaaaaa      >  1:199866/1‑148 (MQ=255)                                                                                                                                                 cAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAActc    <  1:509490/149‑1 (MQ=255)                                                                                                                                                  aaaaTGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTaaaa       >  1:290152/1‑145 (MQ=255)                                                                                                                                                  aaaaTGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTaaaa       <  2:290152/145‑1 (MQ=255)                                                                                                                                                   aaaTGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTg  <  1:87480/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTG  >  NC_000913_3_hsa_tpiA/4542820‑4543114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.