Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA3,946,1400GT46.5% 51.5 / 58.7 44noncoding (2437/2904 nt)rrlC23S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base G (15/8);  new base T (11/9);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT  >  NC_000913_3_hsa_tpiA/3946083‑3946289
                                                         |                                                                                                                                                     
taggacttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTg                                                           <  1:62033/143‑1 (MQ=2)
 aGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGa                                                          >  1:103442/1‑150 (MQ=32)
 aGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGa                                                          <  3:94145/150‑1 (MQ=32)
   gacttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAg                                                        >  2:394005/5‑150 (MQ=2)
   gTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAg                                                        >  3:152055/1‑150 (MQ=32)
    tCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAGAAAAGGAACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGt                                                       >  1:481104/1‑150 (MQ=17)
      ttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGtcg                                                     >  3:173905/2‑148 (MQ=2)
          tGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGCTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGttc                                                 <  4:80424/150‑3 (MQ=2)
              ccGGTGGTTCTGAATTTAAGGGGAAGCGCTGAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAg                                             >  4:177620/1‑150 (MQ=11)
               cGGTGGTTCTGAATGGAAGGGCCAACGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAgg                                            >  2:212375/1‑150 (MQ=17)
                     ttCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATgg                                      <  1:393403/150‑1 (MQ=32)
                       cTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGCAGGTCCCAAGGGTATGGCt                                    >  2:596027/1‑150 (MQ=17)
                       cTGAATGGAAGGGCCATCGCTCAACGGATAAAAGATACTACGGGGATAAAAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCt                                    >  1:243856/1‑150 (MQ=12)
                        cgtgtGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTg                                   >  2:186098/5‑150 (MQ=2)
                        cgtgtGGAAGGGCCGTCGATCAACGGATAAAAGTTACCCCGGGGATACCAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTg                                   >  2:369432/5‑150 (MQ=2)
                          aaTGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGtt                                 >  1:155097/1‑150 (MQ=32)
                          aaTGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGtt                                 >  1:572422/1‑150 (MQ=32)
                            tGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCg                               >  4:52739/1‑150 (MQ=2)
                              gAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcc                             >  4:175320/1‑150 (MQ=2)
                              gAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcc                             >  2:549591/1‑150 (MQ=2)
                              gAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGcc                             <  4:294683/150‑1 (MQ=32)
                              gAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGcc                             <  1:590549/150‑1 (MQ=32)
                               aaGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGccc                            >  2:470602/1‑149 (MQ=2)
                                  ggCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcccatt                         <  3:137126/150‑5 (MQ=2)
                                   gCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAATAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGCTGTCGGCTCCTCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTa                        >  2:590296/1‑150 (MQ=12)
                                     cATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTaaa                      <  3:18149/150‑1 (MQ=32)
                                      gtCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAg                     <  3:353091/149‑1 (MQ=2)
                                       tCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAgc                    <  2:59153/150‑2 (MQ=2)
                                        cGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAgcg                   <  1:558599/150‑3 (MQ=2)
                                         gCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTgg                  >  1:289513/1‑150 (MQ=32)
                                          cTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGt                 <  1:172524/150‑1 (MQ=2)
                                            cAACGGATAAAAGGTACTCCGGGGATAACAGGATGATACCGCACAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCACAGCACAGCCTGGAGGGGAGGTAGGTCCCAAGGGTATGGGTGTTCGCCATTTAAAGTGGTAc               >  1:323216/1‑150 (MQ=11)
                                             aaCGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTAcg              >  2:365040/1‑150 (MQ=2)
                                             aaCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcg              >  4:369230/1‑150 (MQ=32)
                                              aCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcgc             <  3:82293/150‑1 (MQ=32)
                                               cGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcgcg            >  3:277398/1‑150 (MQ=32)
                                                 gATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAg          >  1:223752/1‑150 (MQ=2)
                                                 gATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAg          >  1:603356/1‑150 (MQ=2)
                                                     aaaGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTgg      <  2:300028/150‑1 (MQ=2)
                                                     aaaGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTgg      <  2:244793/150‑1 (MQ=32)
                                                      aaGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTggg     <  2:457772/150‑1 (MQ=2)
                                                       aGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCCTCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGt    <  3:97863/150‑1 (MQ=17)
                                                       aGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGt    >  2:79765/1‑150 (MQ=32)
                                                       aGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGt    >  4:205678/1‑150 (MQ=32)
                                                         gTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGttt  >  4:340177/1‑150 (MQ=32)
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CAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT  >  NC_000913_3_hsa_tpiA/3946083‑3946289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑