Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_hsa_tpiA 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_hsa_tpiA 257908–258676 258683 8–776 71 [0] [0] 71 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 2579070 (0.000)64 (1.030) 53/262 0.2 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_hsa_tpiA 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_hsa_tpiA/257762‑258055
                                                                                                                                                 |                                                                                                                                                    
ctcgtgttaaAAATTTGATAAATTTTGGCAATTTGGTAGAACGGTTGCATCACAACAGGAGATAGCAATGACGTTATCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                                   <  2:857676‑M1/146‑4 (MQ=255)
  tgtgTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgc                                                                                                                                                 >  2:650815‑M1/1‑144 (MQ=255)
      ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                             >  1:992141‑M1/1‑140 (MQ=255)
      ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                             >  1:725839‑M1/1‑140 (MQ=255)
        aaaaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagat                                                                                                                                           <  2:180058‑M1/149‑12 (MQ=255)
           aaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        >  2:911333‑M1/1‑135 (MQ=255)
             tttGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcg                                                                                                                                      >  1:1051768‑M1/1‑133 (MQ=255)
                 cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                                                                  <  2:351272‑M1/149‑21 (MQ=255)
                  tcagttttgcaaCTGTGGTAAAGGAGGTTCATCACCACAGTAGATAGCGATGACGTTACCGAGTGGACACCCGAAGCGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgctaaggtaatcgattct                                                                                                                                 <  2:527364‑M1/145‑22 (MQ=255)
                  tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTGCCGAGTGGACACCCGGAGAGCAGATTGATCAAAAAATTTACCGCAGTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgcttct                                                                                                                                 >  1:744780‑M1/1‑128 (MQ=255)
                      ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             >  2:1025944‑M1/1‑124 (MQ=255)
                      ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             <  1:911333‑M1/149‑26 (MQ=255)
                           ccAATTTGGTAAAACAGTTGCATAACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCCATAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgctt                                                                                                                        >  2:295524‑M1/1‑119 (MQ=255)
                             aatctagtaaaacAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                      <  1:748374‑M1/143‑33 (MQ=255)
                             aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                      >  2:513141‑M1/1‑117 (MQ=255)
                             aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataaacgattctttttcgattgt                                                                                                                      >  1:450275‑M1/1‑117 (MQ=255)
                              aTTTGGTAAAACAGTTGCGTCAGAACAGGAGATAGCAATGACGTTACCGAGTCGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                                                                     <  1:1050181‑M1/149‑34 (MQ=255)
                               ttgggtaaAACAGGTGCCGTACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtct                                                                                                                    <  2:46532‑M1/146‑35 (MQ=255)
                                 tgttaaaACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  2:926719‑M1/146‑37 (MQ=255)
                                     aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTGCATGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                              >  1:1144841‑M1/1‑109 (MQ=255)
                                       aaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtat                                                                                                            <  1:650815‑M1/149‑43 (MQ=255)
                                        aCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTGagtgcaaagataatcgattctttttcgattgtctggctgtttg                                                                                                           >  1:899381‑M1/1‑106 (MQ=255)
                                               cATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    >  2:541701‑M1/1‑99 (MQ=255)
                                                aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattatttttcgattgtctggctgtatgcgtcaacg                                                                                                   >  1:91948‑M1/1‑98 (MQ=255)
                                                aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATAAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacg                                                                                                   >  2:3204‑M1/1‑98 (MQ=255)
                                                     aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaa                                                                                              <  1:513141‑M1/149‑57 (MQ=255)
                                                        aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctt                                                                                                                                >  1:206525‑M1/1‑90 (MQ=255)
                                                        aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctg                                                                                                                                <  2:206525‑M1/112‑23 (MQ=255)
                                                        aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgaatctttttcgattgtctggctgtatgcgccaacgtgaaaccg                                                                                           >  2:741785‑M1/1‑90 (MQ=255)
                                                         ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                                                                          >  2:260590‑M1/1‑89 (MQ=255)
                                                          gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcga                                                                                                                                     >  1:598298‑M1/1‑88 (MQ=255)
                                                          gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcga                                                                                                                                     <  2:598298‑M1/105‑18 (MQ=255)
                                                              tAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            >  2:267325‑M1/1‑84 (MQ=255)
                                                              tAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            <  1:267325‑M1/110‑27 (MQ=255)
                                                                 cAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                                  >  1:975479‑M1/1‑81 (MQ=255)
                                                                            ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAATAGGCCCGTATATTCGTGAAGGTAcgtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaatt                                                                       >  1:67015‑M1/1‑70 (MQ=255)
                                                                                   gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggc                                                                >  2:976325‑M1/1‑63 (MQ=255)
                                                                                   gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggc                                                                >  1:295161‑M1/1‑63 (MQ=255)
                                                                                      acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                             >  2:779519‑M1/1‑60 (MQ=255)
                                                                                      acCCGAAGAGCAGATTGATCAAAAAATTTACAGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                             >  2:371716‑M1/1‑60 (MQ=255)
                                                                                        ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                           >  1:602312‑M1/1‑58 (MQ=255)
                                                                                        ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                            >  1:1106296‑M1/1‑58 (MQ=255)
                                                                                        ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                            <  2:1106296‑M1/116‑59 (MQ=255)
                                                                                         cGAAGAGCAGATTGATCAAAAAATTTACCGCACGAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgg                                                          >  1:285347‑M1/1‑57 (MQ=255)
                                                                                          gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             >  1:716425‑M1/1‑56 (MQ=255)
                                                                                          gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             <  2:716425‑M1/81‑26 (MQ=255)
                                                                                             gagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaaagtgaaacaggacccggaagtgcgtgcattctcgggctggcgaatgg                                                      >  2:460860‑M1/1‑53 (MQ=255)
                                                                                               gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                                    >  2:51377‑M1/1‑51 (MQ=255)
                                                                                                cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagc                                                   >  1:232248‑M1/1‑50 (MQ=255)
                                                                                                    ttGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                               >  2:636423‑M1/1‑46 (MQ=255)
                                                                                                     tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                              <  1:840112‑M1/149‑105 (MQ=255)
                                                                                                     tGAGCAAAAAATTTACGGCACTAGGCTGGGATATTCGGGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                              <  2:17347‑M1/149‑105 (MQ=255)
                                                                                                      gATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaag                                             <  2:1051768‑M1/149‑106 (MQ=255)
                                                                                                       aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgcattctggggctggtggatggagctggaata                                            >  2:262097‑M1/1‑43 (MQ=255)
                                                                                                                tttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatc                                   <  2:285347‑M1/149‑116 (MQ=255)
                                                                                                                   aCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccg                                <  1:51377‑M1/149‑119 (MQ=255)
                                                                                                                     cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtt                              >  2:596296‑M1/1‑29 (MQ=255)
                                                                                                                     cGCACTAGGCCCGTAGATTCGTGAAGGTAagtgcaaagataatcgatgctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaaccccgcc                              <  2:899381‑M1/149‑121 (MQ=255)
                                                                                                                            ggCCCGTATAGTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttaccta                       <  1:122792‑M1/149‑128 (MQ=255)
                                                                                                                             gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctac                      <  1:596296‑M1/149‑129 (MQ=255)
                                                                                                                               ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattttctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  2:126506‑M1/1‑19 (MQ=255)
                                                                                                                                cGTATATTCGTGAAGGTAagtgcaaaggtaatcgattctttttcgatggtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatccggttttgcttacagt                   >  1:164615‑M1/1‑18 (MQ=255)
                                                                                                                                  tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggcagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagtta                 <  1:692903‑M1/149‑134 (MQ=255)
                                                                                                                                      ttCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccag             >  1:617961‑M1/1‑12 (MQ=255)
                                                                                                                                          tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg         <  1:479782‑M1/149‑142 (MQ=255)
                                                                                                                                            aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt       <  1:1122679‑M1/149‑144 (MQ=255)
                                                                                                                                                tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                                >  2:895984‑M1/1‑2 (MQ=255)
                                                                                                                                                tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                                <  1:895984‑M1/72‑71 (MQ=255)
                                                                                                                                                 aagtgcgaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaacccggcagcggaagtgcgtgaattctgggtctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  2:362337‑M1/149‑149 (MQ=255)
                                                                                                                                                 aagtgcaaagataatcgattcttgttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  1:776857‑M1/149‑149 (MQ=255)
                                                                                                                                                 aagtgcaaagataatcgagtctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgggaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  2:91948‑M1/149‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_hsa_tpiA/257762‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: