Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,183,186	G→T	D654Y (GAC→TAC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,183,186	0	G	T	100.0%	234.3 / NA	73	D654Y (GAC→TAC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/40);  total (33/40)

ACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTA  >  NC_000913_3_hsa_tpiA/4183040‑4183330                                                                                                                                                   |                                                                                                                                                 aCCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCAAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTAc                                                                                                                                                <  2:897750/149‑1 (MQ=255)  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACg                                                                                                                                               <  1:236006/149‑1 (MQ=255)         gcaaTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCAc                                                                                                                                        <  2:864585/146‑1 (MQ=255)         gCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGCCCAGGTTGACTACATGTACGTATCCAc                                                                                                                                        >  2:570670/1‑149 (MQ=255)          cTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTGGAAGACCTGGTAACTTGCCGTAGCAAAGACGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGtcccctac                                                                                                                                       >  2:767302/1‑142 (MQ=255)           tATCGAAGAAGGCAAATACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACATCGTAGAAGACCTGGTAACTGGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCAccc                                                                                                                                      <  1:64856/149‑1 (MQ=255)            aTCGAAGAAGGCAACTACGTGATCGCCCAGGCGAACTCCAACTGGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCca                                                                                                                                     <  2:313502/149‑1 (MQ=255)               gaagcaGCCAACCACCTTACCGCCCAAGCCAACTCCAACTTGGATGAAGAAGGCCACCTAGTAGAAGACCTGCTAACTTGCCGTAGCAAAGACGAAACCAGCTTGATCAACCCCGACACGAACCACCACATGTACGTATCCACccacca                                                                                                                                  >  2:242453/1‑149 (MQ=255)                aagaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCCCCcagcag                                                                                                                                 >  1:138155/1‑149 (MQ=255)                   aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAggtg                                                                                                                              <  1:115112/149‑1 (MQ=255)                     ggCAACTACGGTAGCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCAGCGACCAATGTGACTACATGTACGTATCCACCCAGCAggtggt                                                                                                                            >  2:246008/1‑149 (MQ=255)                      gCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTa                                                                                                                           >  2:1086143/1‑149 (MQ=255)                       cAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTAt                                                                                                                          <  2:820120/149‑1 (MQ=255)                         attACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCTGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATcc                                                                                                                        <  2:178082/147‑1 (MQ=255)                                     cccAGGCGAACTCCAACTTGGATGAAGAATGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTCGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTcc                                                                                                            <  1:444722/149‑1 (MQ=255)                                     cccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTcc                                                                                                            <  2:969143/149‑1 (MQ=255)                                      ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                           <  1:192573/149‑1 (MQ=255)                                        aGGCGAACTCCAACTTAGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGCTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGGCGCTGAGACCCTg                                                                                                         >  2:722541/1‑149 (MQ=255)                                             aaCTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATccc                                                                                                    <  1:979557/149‑1 (MQ=255)                                               cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTCCGTCCCTGATCCCGt                                                                                                  >  2:310208/1‑149 (MQ=255)                                                tCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGtt                                                                                                 >  1:632143/1‑149 (MQ=255)                                                tCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGtc                                                                                                 >  2:319723/1‑148 (MQ=255)                                                 ccAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGCCCAGTTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTc                                                                                                <  1:277321/149‑1 (MQ=255)                                                 ccAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGCGGAATCCGTCGGTGCGTCCCTGATCCCATTc                                                                                                >  2:506972/1‑149 (MQ=255)                                                 ccAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGCTGGTATCCGTCGGTTGGTCCCTGCTCCCGTTc                                                                                                >  2:212355/1‑149 (MQ=255)                                                  cAACTCGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTcc                                                                                               <  1:563048/149‑1 (MQ=255)                                                   aatttGAATGAATAATGCCACTTCGTAGAAGACCTGGTAACGTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATTTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                                                                              <  1:87342/146‑1 (MQ=255)                                                   aaCTTGGATGAAGAAGGCCACTTCGTAGAAGCCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                                                                              <  1:99440/149‑1 (MQ=255)                                                   aaCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                                                                              >  1:452178/1‑149 (MQ=255)                                                   aaCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                                                                              <  2:620573/149‑1 (MQ=255)                                                   aaCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAAGCCAGTTTGTTTAGCCGAGACTATGTTTACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                                                                              <  2:297074/149‑1 (MQ=255)                                                    aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTg                                                                                             <  1:612796/149‑1 (MQ=255)                                                       ctgATTAATAAGGCCACTTCGTAGAAGACCAGATAACGTACCGGAGTAAAGGCGAATCCAGCGTGTTCAGCCGCGACCAGGTTTACTACATTTTCGTATCCACCCAGCAGGTGTTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGaa                                                                                          <  2:103763/147‑1 (MQ=255)                                                          aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTa                                                                                                                                             <  1:1000990/95‑1 (MQ=255)                                                          aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTa                                                                                                                                             >  2:1000990/1‑95 (MQ=255)                                                            gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGa                                                                                     >  2:197344/1‑149 (MQ=255)                                                               gatggCCATTTCGTTGATGATCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGGTCATGTTGACTACTTTTTTGTATCCACCCAGCAGGTGGTATCTGTCGGTGCGTTTCTTTTTCTGTTTCTGGAACACGATGa                                                                                  <  2:200182/146‑1 (MQ=255)                                                                aagGCCACTTCGTAGAAGACCTGGTAATTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTTACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGAc                                                                                 <  1:603665/149‑1 (MQ=255)                                                                   gCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGcc                                                                              >  2:872108/1‑149 (MQ=255)                                                                     cACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                                                                            >  1:287819/1‑149 (MQ=255)                                                                        ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCCACCg                                                                         >  2:673787/1‑149 (MQ=255)                                                                        ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCg                                                                         >  2:313791/1‑149 (MQ=255)                                                                        ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCg                                                                         >  1:520059/1‑149 (MQ=255)                                                                        ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTATCGGATCCCGTTCCTGGAACACGATGACGCCAACCg                                                                         <  1:580789/149‑1 (MQ=255)                                                                        ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTAGGTGTGGCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCg                                                                         <  1:580624/149‑1 (MQ=255)                                                                           gTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGc                                                                      <  2:452178/149‑1 (MQ=255)                                                                            tAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGa                                                                                     >  2:60555/1‑133 (MQ=255)                                                                            tAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGa                                                                                     <  1:60555/133‑1 (MQ=255)                                                                                agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                 <  1:1216/149‑1 (MQ=255)                                                                                   ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGCTGACGCCAACCGTGCATTGATgg                                                              >  2:213972/1‑149 (MQ=255)                                                                                         aaCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                        >  1:1124786/1‑149 (MQ=255)                                                                                             tGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGGGGTATCCGTCGTGGCGTCCCTGACCCCGTCCCGGGAACACGATGACGCCAACCGTGCTTTGCGGGGTGCGAACag                                                    >  1:101333/1‑148 (MQ=255)                                                                                              ggcatAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                   <  2:826591/145‑1 (MQ=255)                                                                                               ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCATTTTGACTTCATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGGCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGc                                                  <  1:156995/149‑1 (MQ=255)                                                                                                  tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCGCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAAc                                               <  2:1163266/149‑1 (MQ=255)                                                                                                  tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCACGTTTCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAAc                                               >  1:569822/1‑149 (MQ=255)                                                                                                      aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                           <  1:316872/149‑1 (MQ=255)                                                                                                         ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGAt                                                                                                       <  1:732373/86‑1 (MQ=255)                                                                                                         ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGAt                                                                                                       >  2:732373/1‑86 (MQ=255)                                                                                                         ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                        <  2:731940/149‑1 (MQ=255)                                                                                                            gAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGt                                     >  2:680223/1‑149 (MQ=255)                                                                                                               tCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGGACATGc                                                  >  1:260758/1‑133 (MQ=255)                                                                                                               tCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGc                                                  <  2:260758/133‑1 (MQ=255)                                                                                                               tCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTcc                                  >  1:55729/1‑149 (MQ=255)                                                                                                               tCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTcc                                  <  2:1121212/149‑1 (MQ=255)                                                                                                                 cAGCTCGTTCAGCCGCGACCAGGTTGACTACATTTACGTATCCACCCAGCATCTGGTATCCGTCGGTGCGTCCCTGACCCCGCTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGCCAGGCCGTTCCGa                                <  2:752753/149‑1 (MQ=255)                                                                                                                   gCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                              <  1:1087039/149‑1 (MQ=255)                                                                                                                         tCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgc                        <  2:917458/149‑1 (MQ=255)                                                                                                                           aGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcgc                      >  1:552570/1‑149 (MQ=255)                                                                                                                               gcgACCAGGTTGACTACATGTACGTATCCAACCCGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGAAACGTCAGGCCGGTCCGACTCTGCCCGCTGAt                  >  2:1127201/1‑149 (MQ=255)                                                                                                                                           aCTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACACTGCGCGCAGATAAGCCGCtggtt      >  2:566756/1‑149 (MQ=255)                                                                                                                                             tACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg    <  1:197344/149‑1 (MQ=255)                                                                                                                                               cATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa  >  1:652534/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTA  >  NC_000913_3_hsa_tpiA/4183040‑4183330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑