Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA11,1550CA71.1% 46.2 / 29.0 45G68* (GGA→TGA) yaaWUPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base C (7/6);  new base A (18/14);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTG  >  NC_000913_3_hsa_tpiA/11014‑11296
                                                                                                                                             |                                                                                                                                             
gCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCCGTTTGTTGTGCATAACATCTCCACCAAAATGCTGTAATTCTCAGGCAATc                                                                                                                                        >  2:1003324/1‑149 (MQ=255)
  tCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCCCCAAAATGCTGTAATTCTCAGGCAATCAg                                                                                                                                      >  2:404683/1‑149 (MQ=255)
      tttCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGc                                                                                                                                  <  1:352602/149‑1 (MQ=255)
          aaaCGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCACCTGCCAGt                                                                                                                              >  1:598532/1‑149 (MQ=255)
            aCGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTg                                                                                                                            >  1:341231/1‑149 (MQ=255)
                aGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTgcggc                                                                                                                        <  2:508784/149‑1 (MQ=255)
                   cATCTCTTGTTAGGCGTTGAGCTTCAATCGCTTTGAAACATCGGGCAAATTTGCCGGATACAATTTACCGTGTCCGCGCAGGTTGTTCGCGATACTATCGCCCCCAAAATGCTGTAATTCCCAGGCAATCAGCTGCCAGTTGCGGCGAt                                                                                                                     <  2:204088/149‑1 (MQ=255)
                             tCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAACTGATGTAATTCTCATACAGTCAGCTGCCACTTGCGCACCTTTTGCTCggg                                                                                                           >  2:486578/1‑149 (MQ=255)
                                  tttCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTATCCGCGCAGTTTGTTGGTGATACTCCAAACTTCTCACTGCTGTAATTTTCAGGCAATCAGCTGCCAGTTGGGGCGTGGTTGCTCGGGAGGcc                                                                                                      >  1:770533/1‑149 (MQ=255)
                                     cAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCtt                                                                                                   <  1:545390/149‑1 (MQ=255)
                                      aGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGCTACTATCGCCACCAGAATGCTGTAATTCTCCGGCAATCAGCTGAAAGTTGCGGCGCTGTTGCTCGTGATGCCCTTc                                                                                                  >  2:295584/1‑149 (MQ=255)
                                       gCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTcc                                                                                                 >  1:346881/1‑149 (MQ=255)
                                       gCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCAGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATACCCTTcc                                                                                                 >  1:261568/1‑149 (MQ=255)
                                        cTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCa                                                                                                >  1:843106/1‑149 (MQ=255)
                                          tCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATc                                                                                              <  2:341231/149‑1 (MQ=255)
                                            aaTAGCATTTAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGAGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGa                                                                                            <  2:840348/149‑1 (MQ=255)
                                                      aaaCATCGAGCAAAATGGCCCGATACAATTGGCCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCAACCAAAATGCTGTAATTCTAAGGCAATCAGCTACCAGTTGCGGCGATGTTGCTCGGGATGCACTTCCATCGGTTTAAACAGt                                                                                  >  2:854346/1‑149 (MQ=255)
                                                              aGCAGAAAGGCCCGATACCATCCATCGGGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTgcg                                                                          >  2:1015956/1‑149 (MQ=255)
                                                               gCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTgcgc                                                                         >  1:836733/1‑149 (MQ=255)
                                                                   aaTGGGCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCa                                                                     <  1:117410/149‑1 (MQ=255)
                                                                           gATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTg                                                             <  1:355309/149‑1 (MQ=255)
                                                                             tACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCAACTCAATGGGGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGga                                                           <  1:295584/149‑1 (MQ=255)
                                                                              aCAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCGACCGAGTGCGGTTATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGgag                                                          <  2:1005954/149‑1 (MQ=255)
                                                                               cAATTTACCGTGTCCGCGCAGGTTGTTGGCTATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTAGCGCAACAGTACGCTGgaga                                                         <  2:151089/149‑1 (MQ=255)
                                                                                aaTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCggg                                                                                                           >  1:636125/1‑98 (MQ=255)
                                                                                aaTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCggg                                                                                                           <  2:636125/98‑1 (MQ=255)
                                                                                 aTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTTCGCTGGGGAgg                                                       >  1:79308/1‑149 (MQ=255)
                                                                                    tACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGa                                                    <  2:346881/149‑1 (MQ=255)
                                                                                       cGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAAGTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGCTTTAAAAAGTTCGTTGCGCATCAGTAGGCTGGAGAGGCGAGtt                                                 >  1:250446/1‑149 (MQ=255)
                                                                                        gcgtCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCACGTAGGTACGCTGGAGAGTCGAGttt                                                <  2:598532/147‑1 (MQ=255)
                                                                                           tCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATAAGTGCGCTGGAGAGGCGAGTTTAGc                                             >  2:631550/1‑149 (MQ=255)
                                                                                           tCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGc                                             <  1:76145/149‑1 (MQ=255)
                                                                                              gcgcAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCtt                                          >  1:703882/1‑149 (MQ=255)
                                                                                                    tttGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGTAGAGGCGAGTTTTGCCTTTTTCAt                                    <  1:1003324/149‑1 (MQ=255)
                                                                                                         tgGCGATACTATCGCCACCAAAATGCTGTCACTCTCCGGCAACCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATgg                               <  2:843106/149‑1 (MQ=255)
                                                                                                           gCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGa                                                                                                          >  2:833142/1‑72 (MQ=255)
                                                                                                           gCGATACTATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGa                                                                                                          <  1:833142/72‑1 (MQ=255)
                                                                                                             gtgattATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGa                           <  2:787246/144‑1 (MQ=255)
                                                                                                                  tATCGCCACCAAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGCGGCGAGTTTTTCCTTTTTCATTATGGGTGAGCAAt                      >  2:456436/1‑149 (MQ=255)
                                                                                                                          ccaAAATGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGaa              >  2:397068/1‑149 (MQ=255)
                                                                                                                                tGCTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCATTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGc        <  1:780031/149‑1 (MQ=255)
                                                                                                                                  cTGTAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCa      >  1:846933/1‑149 (MQ=255)
                                                                                                                                    gTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAAc    >  2:505596/1‑149 (MQ=255)
                                                                                                                                     tAATTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCACCt   >  2:630750/1‑149 (MQ=255)
                                                                                                                                      aaTTCTCAGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTg  >  2:165919/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                             
GCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTG  >  NC_000913_3_hsa_tpiA/11014‑11296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑