Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	111,726	C→T	A41T (GCT→ACT)	yacG ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	111,726	0	C	T	80.0%	106.3 / 22.5	58	A41T (GCT→ACT)	yacG	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base C (6/5);  new base T (14/30);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

ATTTGCACAAGATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCA  >  NC_000913_3_hsa_tpiA/111580‑111869                                                                                                                                                   |                                                                                                                                                aTTTGCACAAGATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGtcc                                                                                                                                               >  2:98490/1‑149 (MQ=255) aTTTGCACAAGATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGccc                                                                                                                                               >  1:29224/1‑149 (MQ=255)     gCACAAGATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATtc                                                                                                                                           <  1:345851/149‑1 (MQ=255)       acaAGATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGATGGGGGTTCGTTTTTCTTCAGCAGTCCATtctc                                                                                                                                         >  1:925437/1‑149 (MQ=255)         aaGATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGCTTCGTTGTTCTTCAGCAGGCCATTCCCCg                                                                                                                                       >  2:461003/1‑149 (MQ=255)           gATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAg                                                                                                                                     <  2:6959/149‑1 (MQ=255)           gATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAg                                                                                                                                     >  2:738161/1‑149 (MQ=255)           gATGCCCGAGGCGCCGCCGTCCGCTGCGGATCATGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAg                                                                                                                                     <  2:741872/149‑1 (MQ=255)             tGCCTGATGCGACGGTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGt                                                                                                                                   <  2:235210/149‑1 (MQ=255)                cTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGa                                                                                                                                >  1:394273/1‑149 (MQ=255)                   aTGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCa                                                                                                                             >  1:805194/1‑149 (MQ=255)                     gCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGTTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGc                                                                                                                           >  2:591108/1‑149 (MQ=255)                      cGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTCCAGCAGTCCATTCTCCGAGGTCGATCAGCt                                                                                                                          <  1:679466/149‑1 (MQ=255)                             gTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACg                                                                                                                   <  2:394273/149‑1 (MQ=255)                                cgcgTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGTTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGttt                                                                                                                <  1:591108/149‑1 (MQ=255)                                  cgTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTgg                                                                                                              <  1:98490/149‑1 (MQ=255)                                           aGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGTATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTTGAGCAAAATg                                                                                                     >  2:571654/1‑149 (MQ=255)                                             gCCTAAGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGc                                                                                                   <  1:493613/149‑1 (MQ=255)                                                   gTGGGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAAc                                                                                             <  2:196792/149‑1 (MQ=255)                                                    tGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACg                                                                                            <  1:283446/149‑1 (MQ=255)                                                      cGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACggg                                                                                          <  1:281692/149‑1 (MQ=255)                                                       gtcATAAGACAAATGTAACTGCATTGATACTTCGCTCCAGTCATCGCTTTCGGGAAAATAGCCACTTATAGGGATTCAATATTATTAAGCAGATCATTCTACAAGGTCACTCATCTGACAAAGTTTCGATCAACATGAACGACACggac                                                                                         >  2:278372/3‑147 (MQ=255)                                                        gaataaGACAAATGTCACTGCTTTGGTTTTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCt                                                                                        <  2:374649/144‑1 (MQ=255)                                                        gCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCt                                                                                        >  1:167210/1‑149 (MQ=255)                                                        gCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCt                                                                                        <  2:548285/149‑1 (MQ=255)                                                              gACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTc                                                                                  >  2:472949/1‑149 (MQ=255)                                                               aCAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTcc                                                                                 <  2:805194/149‑2 (MQ=255)                                                               aCAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCa                                                                                 <  2:167210/149‑1 (MQ=255)                                                                 aaaTGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCAcc                                                                               <  2:854281/149‑1 (MQ=255)                                                                      tCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGAGCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAACATGGCCGCAACGAGCTGGTTTCACCCcaca                                                                          >  1:286695/1‑149 (MQ=255)                                                                       cACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCacac                                                                         >  1:292355/1‑149 (MQ=255)                                                                          tGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCAcaccac                                                                      >  2:294807/1‑149 (MQ=255)                                                                            cTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCt                                                                                        >  1:27063/1‑129 (MQ=255)                                                                            cTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCt                                                                                        <  2:27063/129‑1 (MQ=255)                                                                            cTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCg                                                                    <  1:507551/149‑1 (MQ=255)                                                                               gggtctttcGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGttt                                                                 <  2:280550/143‑1 (MQ=255)                                                                                 gTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTc                                                               <  2:784587/149‑1 (MQ=255)                                                                                 gTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTc                                                               <  1:777273/149‑1 (MQ=255)                                                                                  ttcttcGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCAcca                                                                              <  2:132902/133‑2 (MQ=255)                                                                                  ttcttcGCTCCAGTCATCGCTTTCGGAAAGATCCCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCAcca                                                                              >  1:132902/1‑132 (MQ=255)                                                                                        gCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGGCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAgg                                                        <  2:436731/149‑1 (MQ=255)                                                                                          tCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGc                                                                                                   <  2:449228/104‑1 (MQ=255)                                                                                          tCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGc                                                                                                   >  1:449228/1‑104 (MQ=255)                                                                                           ccAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGTATTCTTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAATGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTg                                                     <  2:196333/149‑1 (MQ=255)                                                                                            cattCATCGCTTTCGTAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCACTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTgg                                                    <  1:352528/146‑1 (MQ=255)                                                                                                  tCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAAtt                                              <  1:606595/149‑1 (MQ=255)                                                                                                    gCTTGCGGAAAGATCGCAACTGATGATGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCa                                            >  2:800801/1‑149 (MQ=255)                                                                                                      tttCGGAAAGATCGCCAGTGCTGGGGATTCGTTTTTTTTCAGGAGTCCATTGTCCGCGGTCGATCAGCTGGCAAATTTTGAATCAAACTGGCCGAAACGGGGTGATTTCACCCCACACCACCGTTGTCCCACAGGTGTGGCAATTCAca                                          >  2:596235/1‑148 (MQ=255)                                                                                                      tttCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCAcc                                          >  1:526261/1‑149 (MQ=255)                                                                                                                   gCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAg                                                                                                            >  2:116082/1‑70 (MQ=255)                                                                                                                   gCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAg                                                                                                            <  1:116082/70‑1 (MQ=255)                                                                                                                         gctgGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGTTGACAACGTTTGGAGCAGAATGGCCGAAACGGGCTGAGTTCACCCCACACCACCGTTTTCCCGCAGGTTGCGCAATTCACCGTTATAGTTTCTGACAttt                       <  2:947865/149‑1 (MQ=255)                                                                                                                         gctgGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACAttt                       <  2:535348/149‑1 (MQ=255)                                                                                                                          ctgGGGATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACAtttt                      <  1:701043/149‑1 (MQ=255)                                                                                                                            ggggATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTa                    <  1:701578/149‑1 (MQ=255)                                                                                                                            ggggATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTa                    <  1:738161/149‑1 (MQ=255)                                                                                                                               gATTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTc                 <  1:294807/149‑1 (MQ=255)                                                                                                                                aTTCGTTTTTCTTCAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTcc                >  2:75567/1‑149 (MQ=255)                                                                                                                                         tcttcAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACa       <  2:292355/149‑1 (MQ=255)                                                                                                                                            tcAGCAGTCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGCGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACTTTTTTACTCCTTCCTAACAGGc    >  2:87729/1‑149 (MQ=255)                                                                                                                                              agcagcCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAACATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCa  <  1:800801/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                ATTTGCACAAGATGCCTGATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACAAATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCA  >  NC_000913_3_hsa_tpiA/111580‑111869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑