New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 2544028 =54 (1.380)4 (0.110) 3/270 5.5 7.2% coding (596/792 nt) ucpA furfural resistance protein, putative short‑chain oxidoreductase
?NC_000913_3_hsa_tpiA 2544050 = 52 (1.390)coding (574/792 nt) ucpA furfural resistance protein, putative short‑chain oxidoreductase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCGATCCTGGCGAAACGGCGTACGCCTTAACGAAAGCGGCGATTGTTGGCCTGACAAAATCGCTGGCGGTGGAGTACGCGCAGTCTGGTATTCGCGTTAACGCCATTTGCCCGGGATACGTGCGCACACCAATGGCGGAAAGCATTGCCCGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_hsa_tpiA/2544182‑2544028
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgccaTTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCA  >  NC_000913_3_hsa_tpiA/2544050‑2544195
                                                                                                                                                                                                                                                                                                              
GCCGATCCTGGCGAAACGGCGTACGCCTTAACGAAAGCGGCGATTGTTGGCCTGACAAAATCGCTGGCGGTGGAGTACGCGCAGTCTGGTATTCGCGTTAACGCCATTTGCCCGGGATACGTGCGCACACCAATGGCGGAAAGCATTGCC                                                                                                                                                          >  2:684084/1‑150
   GATCCTGGCGAAACGGCGTACGCCTTAACGAAAGCGGCGATTGTTGGCCTGACAAAATCGCTGGCGGTGGAGTACGCGCAGTCTGGTATTCGCGTTAACGCCATTTGCCCGGGATACGTGCGCACACCAATGGCGGAAAGCATTGCCCGC                                                                                                                                                       >  1:561158/1‑150
                                                                                                                                    ATGGCGGAAAGCATTGCCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGC                                                                                              <  1:308342/78‑1
                                                                                                                                    ATGGCGGAAAGCATTGCCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGC                                                                                              >  2:308342/1‑78
                                                                                                                                    ATGGCGGAAAGCATTGCCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGC                                                                                                              >  1:519040/1‑62
                                                                                                                                    ATGGCGGAAAGCATTGCCCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGC                                                                                                              <  2:519040/62‑1
                                                                                                                                                     CCGCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTCCGCCGTTTCGCCAGGATCGCCACCACTATCAC    >  2:157119/1‑150
                                                                                                                                                       GCCATTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATCTCACCA  >  2:469819/1‑150
                                                                                                                                                                                                                                                                                                              
GCCGATCCTGGCGAAACGGCGTACGCCTTAACGAAAGCGGCGATTGTTGGCCTGACAAAATCGCTGGCGGTGGAGTACGCGCAGTCTGGTATTCGCGTTAACGCCATTTGCCCGGGATACGTGCGCACACCAATGGCGGAAAGCATTGCCCGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_hsa_tpiA/2544182‑2544028
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgccaTTGGTGTGCGCACGTATCCCGGGCAAATGGCGTTAACGCGAATACCAGACTGCGCGTACTCCACCGCCAGCGATTTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCA  >  NC_000913_3_hsa_tpiA/2544050‑2544195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.