New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	2110440 =	25 (0.640)	3 (0.080)	3/274	5.6	9.2%	coding (580/882 nt)	rfbA	glucose‑1‑phosphate thymidylyltransferase
	?	NC_000913_3_hsa_tpiA	2110468 =	35 (0.920)					coding (552/882 nt)	rfbA	glucose‑1‑phosphate thymidylyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCA  >  NC_000913_3_hsa_tpiA/2110324‑2110612                                                                                                                                                 |                                                                                                                                                 aCTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCAc                                                                                                                                             <  1:402452/150‑1 (MQ=255)   tctGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGGCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGGTTCAGGTTTTTCGCCATCTGAACCAcgt                                                                                                                                           >  2:468035/1‑150 (MQ=255)        tgaGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTa                                                                                                                                      >  2:575496/1‑150 (MQ=255)               ccGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATTTGAACCACGTCGTTATCATaaa                                                                                                                               >  2:636579/1‑150 (MQ=255)                ggtgtGCAGCCACGCGTAGCCACGCGCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATaaaa                                                                                                                              <  2:343029/149‑1 (MQ=255)                cctttCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATaaaa                                                                                                                              <  2:343026/146‑1 (MQ=255)                   gtCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTa                                                                                                                           <  2:311413/150‑1 (MQ=255)                              cgTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTaa                                                                                                                >  1:264347/1‑150 (MQ=255)                                       gCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCATCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATACAAGTACACACCTGTAACgcggaaca                                                                                                       >  2:516685/1‑143 (MQ=255)                                            atcatcGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGGTATCATACAAGTACAGACCTGTAACGGCGTAATTActc                                                                                                  >  1:1104/1‑150 (MQ=255)                                                tcGCGAAAGACCGACGTCCCTGCCCAAGATAAATACGGTCACTAGCTTGAATTTCGAACTCACCACGTGCAGACGGCTTCAAGTTTTGCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGGCCTGTAACGGCGTAATTACTCTTTg                                                                                              <  1:364050/150‑1 (MQ=255)                                                        gacagacGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAAc                                                                                      <  1:559089/150‑1 (MQ=255)                                                          cagacGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACgg                                                                                    >  1:200588/1‑150 (MQ=255)                                                           agacGTCCCTGCTGAAGATAAATACTGTTAATATCTGGAAGTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGAAACGGCGTAATTACTCTTTGGTTCTAACGGt                                                                                   <  2:553509/150‑1 (MQ=255)                                                                tCCCTGCTCAAGATAAATGCGGTTAATATTTGTAATTTCTAACTCACCACGTGGAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTttc                                                                              <  2:188709/150‑1 (MQ=255)                                                                                  aCGGGTATTATCTGGAATTTCTAACTCCCCACGTGCAGACGGCTGCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTGCTAACGGTTTTTCTTCCAGACTGATTGCCGt                                                            <  2:88232/150‑1 (MQ=255)                                                                                                 aaTTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCaaa                                             <  2:200588/150‑1 (MQ=255)                                                                                                         aCTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGa                                     >  1:80937/1‑150 (MQ=255)                                                                                                           tCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTAc                                                                                                                          >  2:332119/1‑63 (MQ=255)                                                                                                           tCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTAc                                                                                                                          <  1:332119/63‑1 (MQ=255)                                                                                                           tCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGaca                                   >  2:285572/1‑150 (MQ=255)                                                                                                             accacGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACAcc                                 >  2:362665/1‑150 (MQ=255)                                                                                                                       gACGGCTTCAAGTTTCTCGCCAGCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCt                       <  1:468035/150‑1 (MQ=255)                                                                                                                        aCGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTg                      <  1:632453/150‑1 (MQ=255)                                                                                                                        aCGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTg                      >  1:489984/1‑150 (MQ=255)                                                                                                                         cGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTCCCGTTTTTATCAAACTCAACGACACCATAGCGTTCTgg                     <  1:217789/150‑1 (MQ=255)                                                                                                                           gCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGAt                   <  1:575496/150‑1 (MQ=255)                                                                                                                           gCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGAt                   <  1:285572/150‑1 (MQ=255)                                                                                                                           gCTTAAAGTTTTTCGTCATCTGAATCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGAt                   <  2:1104/150‑1 (MQ=255)                                                                                                                               gaaGTTTTCCGCCATCTGAACCACGTCGTGATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCAtt               <  2:324028/149‑1 (MQ=255)                                                                                                                                aaGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTa              <  2:514252/150‑1 (MQ=255)                                                                                                                                 aGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTaa                                                                                                                <  1:321102/51‑1 (MQ=255)                                                                                                                                 aGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTaa                                                                                                                >  2:321102/1‑51 (MQ=255)                                                                                                                                  tgttttCGCCATCGGAACCACGTCGGTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAAc            <  1:574932/148‑1 (MQ=255)                                                                                                                                            aTCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTCATTACTCTTTGATTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCATTCTGGATAATTAACGTGAtcagca  >  2:306738/1‑145 (MQ=255)                                                                                                                                                 |                                                                                                                                                 ACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCA  >  NC_000913_3_hsa_tpiA/2110324‑2110612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.