Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	3,440,923	C→A	G36V (GGC→GTC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	3,440,923	0	C	A	100.0%	229.5 / NA	70	G36V (GGC→GTC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/35);  total (35/35)

TTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGA  >  NC_000913_3_hsa_tpiA/3440779‑3441063                                                                                                                                                 |                                                                                                                                             ttGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACAATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGACTACGGCGCAGTCCGTTACCCTGAGAACGGACaac                                                                                                                                          >  2:923950/1‑148 (MQ=255)    aGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATCGACAAAGcc                                                                                                                                       >  1:870140/1‑149 (MQ=255)              ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTaaa                                                                                                                             <  2:808487/149‑1 (MQ=255)              ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTaaa                                                                                                                             <  2:156270/149‑1 (MQ=255)               cAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAg                                                                                                                            >  2:557194/1‑149 (MQ=255)               cAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAg                                                                                                                            <  1:457747/149‑1 (MQ=255)                 ggATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGc                                                                                                                          <  1:521003/149‑1 (MQ=255)                   atatCGTCCTGAACACCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTc                                                                                                                        <  2:4207/149‑1 (MQ=255)                      tCTTCCTGAACGCCTTCCTTGGCGCTGTACTCATGTAGTACACCATCAATTTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAg                                                                                                                     <  2:714620/149‑1 (MQ=255)                       ctgccTGAGCGCCTTCTGTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                    <  1:1028877/146‑1 (MQ=255)                       cTTCCTTAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGCATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                    <  2:949510/149‑1 (MQ=255)                       cTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                    <  1:537612/149‑1 (MQ=255)                        ttCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGACATCAGAATACGGCGCATTGCGTTGCCCGGCGTATGGATGAAGCCACGCTCTAAAGGCTCAAggg                                                                                                                   >  1:601538/1‑149 (MQ=255)                        ttCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCCAAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCATCGCTCTAAAGGATCAAggg                                                                                                                   >  1:109916/1‑149 (MQ=255)                          ccTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                 >  1:100650/1‑149 (MQ=255)                            tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                               >  1:417545/1‑149 (MQ=255)                                 gCCTTCGTTGGTCCCGCTCTCACGTAGCGCACCATCAATCTCATCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTgg                                                                                                          <  2:163395/149‑1 (MQ=255)                                       tttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcg                                                                                                    >  2:286079/1‑149 (MQ=255)                                            tGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                               <  1:814413/149‑1 (MQ=255)                                              cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGACTACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAgg                                                                                                                           >  2:459464/1‑119 (MQ=255)                                              cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAgg                                                                                                                           <  1:459464/119‑1 (MQ=255)                                                 tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                          <  2:549878/149‑1 (MQ=255)                                                    tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTg                                                                                       >  2:516136/1‑149 (MQ=255)                                                    tCATGTAGTACACCATAAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGGATGGACAAAGCCACGCTCGAGAGGCTCAAGGGTCACCTTGGCGTGCCTCGAAGCCACTTg                                                                                       >  2:924926/1‑149 (MQ=255)                                                      aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAAGCTCCAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                     >  2:276925/1‑149 (MQ=255)                                                       tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                    <  1:516136/149‑1 (MQ=255)                                                               acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCaa                                                                            <  1:222719/149‑1 (MQ=255)                                                                 catcatTCTCATGCTCGGTCAGCGTGCAACCCGGCATCGATGCGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                          <  2:313720/149‑1 (MQ=255)                                                                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCACAGTATGGACAAAGCCACACTCTACAGGCTCAGGGGTCACCTTGGCGTGCGTCGAACTCGCTTACTCGATATCAccc                                                                          >  1:201776/1‑149 (MQ=255)                                                                  aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                         >  2:515376/1‑149 (MQ=255)                                                                  aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                         >  1:866470/1‑149 (MQ=255)                                                                  aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                         >  2:1030994/1‑149 (MQ=255)                                                                   tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                    >  2:469575/1‑105 (MQ=255)                                                                   tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                    <  1:469575/105‑1 (MQ=255)                                                                   tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAg                                                                        <  2:686159/149‑1 (MQ=255)                                                                             ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCGCGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTGACTTGCTCGATATCAACCCGGCGCGGtttt                                                              >  2:239825/1‑149 (MQ=255)                                                                               tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAg                                                            <  1:793085/149‑1 (MQ=255)                                                                                   tCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGCTATCAACCAGGCGCGATTTTAGAAAc                                                        >  1:97068/1‑149 (MQ=255)                                                                                     aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                      >  1:544452/1‑149 (MQ=255)                                                                                      ccGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                     <  1:1030994/149‑1 (MQ=255)                                                                                      ccGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                     <  1:515376/149‑1 (MQ=255)                                                                                         cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGACACTCTGTc                                                  >  1:533266/1‑149 (MQ=255)                                                                                          gcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGGAACTCTGTca                                                 >  1:191037/1‑149 (MQ=255)                                                                                            aaCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                               <  2:544452/149‑1 (MQ=255)                                                                                              cccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                               >  2:684008/1‑99 (MQ=255)                                                                                              cccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                               <  1:684008/99‑1 (MQ=255)                                                                                                     tCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                      <  1:557194/149‑1 (MQ=255)                                                                                                        aTGAGAGCAGAATATGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                   <  2:923440/149‑1 (MQ=255)                                                                                                            gagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTgtgt                               >  1:78414/1‑149 (MQ=255)                                                                                                             agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                              >  2:995060/1‑149 (MQ=255)                                                                                                                       ggCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                    <  2:773347/149‑1 (MQ=255)                                                                                                                       ggCGCAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                    >  2:700159/1‑149 (MQ=255)                                                                                                                          gcAGTGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTGGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCACTTTGGTAc                 >  2:536620/1‑149 (MQ=255)                                                                                                                            aGTGCGTTACCCCGGGTATGGACAAAGCCACGCTCTAAAGGCTCAAAGGTCACCTTGGCGTGCGCCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAGCTCTGTCCCAAAACCCTGCATTCTGTCCTCTCTTTGGTACtt               >  2:66107/1‑148 (MQ=255)                                                                                                                              tGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg             >  2:370373/1‑149 (MQ=255)                                                                                                                              tGCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg             <  1:339461/149‑1 (MQ=255)                                                                                                                               gCGTTACTCAGAGGAGGGACAAAGCCACGAGCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGc            <  2:722044/149‑1 (MQ=255)                                                                                                                               gCGTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGc            <  2:724524/149‑1 (MQ=255)                                                                                                                                 gTTACCCAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGCGCGTCGATCTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt          <  1:923950/149‑1 (MQ=255)                                                                                                                                 gTTACCCACACTCTGGACAAAGCCTCGATCTAACGGCTCAAGGGTCACCTTGGCCTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt          <  2:288699/149‑1 (MQ=255)                                                                                                                                     cccAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt      >  1:672688/1‑149 (MQ=255)                                                                                                                                      ccAGTGTATGGACAAAGCCACGCTCTAAAGGCCCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt     <  1:49622/149‑1 (MQ=255)                                                                                                                                      ccAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATCTCACCCAGAAGCGGTGTTGGAAACTCTGTCACAGCACCCTGCATCGTGTCCTCTCTTTCGTACTAACCTTTACtt     >  2:377285/1‑149 (MQ=255)                                                                                                                                      ccAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAg                                                            <  2:656238/94‑1 (MQ=255)                                                                                                                                      ccAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAg                                                            >  1:656238/1‑94 (MQ=255)                                                                                                                                      ccAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt     >  1:476474/1‑149 (MQ=255)                                                                                                                                      ccAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt     >  1:359219/1‑149 (MQ=255)                                                                                                                                      ccAGAGTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt     >  2:883439/1‑149 (MQ=255)                                                                                                                                         gagTATGGACAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa  <  1:710655/149‑1 (MQ=255)                                                                                                                                         gagTATGAACAAAGCCCCGCGATAAAGGCTCAACGGTCACTTCGGACGGCGTCTATTTCACGTGGTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa  <  2:430216/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                             TTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGA  >  NC_000913_3_hsa_tpiA/3440779‑3441063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑