Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,106,741	A→T	S162S (TCT→TCA)	wbbH ←	O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,106,741	0	A	T	97.3%	252.1 / ‑5.8	75	S162S (TCT→TCA)	wbbH	O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (38/35);  minor base C (1/1);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAA  >  NC_000913_3_hsa_tpiA/2106599‑2106881                                                                                                                                                |                                                                                                                                             tATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCaa                                                                                                                                        >  2:898646/1‑149 (MQ=255)   ttAAGACGTACGAAGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAAATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCaaat                                                                                                                                      <  2:28600/149‑1 (MQ=255)    tAACACCTACGAATAATGCATAAGAGATGATAACCATAAAGACAATTTTCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCaaata                                                                                                                                     <  1:786588/149‑1 (MQ=255)                tGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCaaaa                                                                                                                         <  1:132800/149‑1 (MQ=255)                   aTGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAGGCAAAagta                                                                                                                      >  1:384958/1‑149 (MQ=255)                       aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGAc                                                                                                                                           <  1:598227/124‑1 (MQ=255)                       aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGAc                                                                                                                                           >  2:598227/1‑124 (MQ=255)                         aaGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATCATTCCAAACATGCATCAACTTCAGAAATAGCACC‑TTCTTCGTTTACTTTGCTTGTTAGGATTAAATATTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGa                                                                                                                >  1:218613/1‑149 (MQ=255)                           gagaTGATAACCATAAAGACAATTTGATTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAAAAAGTAAAC‑TAGAAATTTGATGTATGGTTGTATGAATAAGTTTTTTTGACAAAATAAATAAAGAAAAAGTAGTTAGact                                                                                                              >  2:396558/1‑147 (MQ=255)                               tgatAACCATAAAGACAATTGGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAtt                                                                                                          <  1:161339/149‑1 (MQ=255)                               tcacaaCCATCAAAACAATGTGCTTACCACTATTCAGTATAAATGAAACTAAGAATACAATAAAAACAAGTAAAG‑AAAATGTTTTAGTTAACTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAtt                                                                                                          <  1:306773/145‑1 (MQ=255)                               agatACCCAAAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAtt                                                                                                          <  2:589147/148‑1 (MQ=255)                                gatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGattgattg                                                                                                         >  2:931667/1‑149 (MQ=255)                                 atAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTCAAACGACCAATACAATACAAACAAGTAAAG‑CAAATCACATATTCAACTTTATCGAATTAAATGTTGACGACCTTATAACTAATTCAAAAGAAGTTAGAATCATTgg                                                                                                        >  2:919794/1‑149 (MQ=255)                                     ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGc                                                                                                    >  2:60094/1‑149 (MQ=255)                                           aGACAATTTGCTTACCAGTATTCAGTATGATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGAATGTAt                                                                                              >  2:673200/1‑149 (MQ=255)                                                    tcTTACCAGTATTCAGTATAATTGCAAAGATTAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGaaa                                                                                     <  1:932863/148‑1 (MQ=255)                                                         ccAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTATTTACCTTTGTATTAGTAAATTTTTTCGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAt                                                                                                           <  2:959178/122‑1 (MQ=255)                                                         ccAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAt                                                                                                           >  1:959178/1‑122 (MQ=255)                                                         ccAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCt                                                                                >  1:333729/1‑149 (MQ=255)                                                         ccAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCt                                                                                <  1:450581/149‑1 (MQ=255)                                                         ccAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATGAGTAAATTTTTTTGACCAAAGAAATAAAGCAAAAGTAGGTAGAATGATTGACTGCATGTATGATGAGAAATTTCt                                                                                >  1:90550/1‑149 (MQ=255)                                                          cAGTATTCAGTATAATTGCAAAGATGGATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                               <  1:494114/149‑1 (MQ=255)                                                          cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                               >  1:344703/1‑149 (MQ=255)                                                            gTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTTATG‑TTACTGATTTACTTACATTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGa                                                                             <  1:208143/149‑1 (MQ=255)                                                            gTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGa                                                                             <  1:269910/149‑1 (MQ=255)                                                            gTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTAGCTGCATGTATGCTAAGAAATTTCTTgt                                                                             >  2:208143/1‑148 (MQ=255)                                                             tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTAt                                                                                              <  2:938987/131‑1 (MQ=255)                                                             tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTAt                                                                                              >  1:938987/1‑131 (MQ=255)                                                             tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                            >  2:1083067/1‑149 (MQ=255)                                                               ttCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                          <  1:40904/149‑1 (MQ=255)                                                                   gTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCt                                                                      >  2:427357/1‑149 (MQ=255)                                                                    tataATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAagtagt                                                                                                                    <  2:190996/102‑1 (MQ=255)                                                                    tataATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAagtagt                                                                                                                    >  1:190996/1‑102 (MQ=255)                                                                    tataATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCtt                                                                     <  2:755589/149‑1 (MQ=255)                                                                       aaTTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATATGTCTTCaa                                                                  >  1:328757/1‑149 (MQ=255)                                                                         ttGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACATTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGACTGATTGGCTGCATGTATGATGAGAAATTTCTTGATATGTATTCAACa                                                                >  1:175074/1‑149 (MQ=255)                                                                            cAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCCTGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                             <  2:963192/149‑1 (MQ=255)                                                                            cAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                             <  1:898646/149‑1 (MQ=255)                                                                             aaaGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAg                                                            >  1:886604/1‑149 (MQ=255)                                                                             aaaGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAg                                                            >  2:21561/1‑149 (MQ=255)                                                                              aaGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGc                                                           <  1:469760/149‑1 (MQ=255)                                                                                gATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAt                                                         <  1:154785/149‑1 (MQ=255)                                                                                      tACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                   >  1:504838/1‑149 (MQ=255)                                                                                       aCAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATc                                                              <  1:338377/137‑1 (MQ=255)                                                                                       aCAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATc                                                              >  2:338377/1‑137 (MQ=255)                                                                                         aaTAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCa                                                >  1:188465/1‑149 (MQ=255)                                                                                         aaTAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCa                                                >  1:989941/1‑149 (MQ=255)                                                                                         aaTAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGAATCTAATCGGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCa                                                >  1:85586/1‑149 (MQ=255)                                                                                           tAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaaa                                              <  2:504838/149‑1 (MQ=255)                                                                                              aaaCAAGTAAAGCAAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAACTAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGTATCTCTTATCAAAtt                                            <  1:671442/149‑1 (MQ=255)                                                                                              aaaCAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTgg                                                                                                        <  1:434177/88‑1 (MQ=255)                                                                                              aaaCAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTgg                                                                                                        >  2:434177/1‑88 (MQ=255)                                                                                              aaaCAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTc                                           >  1:600688/1‑149 (MQ=255)                                                                                               aaCAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCa                                          >  2:736843/1‑149 (MQ=255)                                                                                                 cAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCata                                        <  1:60094/149‑1 (MQ=255)                                                                                                     taaag‑taaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGc                                    <  2:886604/149‑1 (MQ=255)                                                                                                        ag‑taaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa                                 <  1:676854/149‑1 (MQ=255)                                                                                                        ag‑taaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa                                 <  1:427357/149‑1 (MQ=255)                                                                                                              aTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCCAAAGTAGTTATAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATACCtaattaag                            >  2:43816/1‑149 (MQ=255)                                                                                                                   ttacttacCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGCGAAATTTATTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGATAGt                       >  1:9122/1‑149 (MQ=255)                                                                                                                       ttacCTTTGTATTAGTAAATTTTTTTTTTTTAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCg                   >  2:607538/1‑149 (MQ=255)                                                                                                                           ctatttATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATATCTAAGTAAGCTACTCCCGAACt               >  2:469215/6‑149 (MQ=255)                                                                                                                           cTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAAAAGCAGCATGTATTCTGAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt               >  1:697221/1‑149 (MQ=255)                                                                                                                            ttttTATTAGTAAATTTTTATTACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGTCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg              <  2:175074/149‑1 (MQ=255)                                                                                                                             ttGTATTAGTAAATTTTTTTGACTAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg             <  2:306683/149‑1 (MQ=255)                                                                                                                             ttGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg             <  1:736843/149‑1 (MQ=255)                                                                                                                             ttGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg             <  2:1077324/149‑1 (MQ=255)                                                                                                                                  ttAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAACTTCGTATAGCTAAGTAAGCTAGTCCCGAACTGGTAGtt        >  1:302087/1‑149 (MQ=255)                                                                                                                                     gTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCt                                                                                >  2:24418/1‑74 (MQ=255)                                                                                                                                     gTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCt                                                                                <  1:24418/74‑1 (MQ=255)                                                                                                                                     gTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa           >  1:643206/1‑143 (MQ=255)                                                                                                                                     gTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa           <  2:643206/143‑1 (MQ=255)                                                                                                                                      tAAATTTTTTTGACCAAATAAATAAAGCAACCGTCGGCAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACtt    >  1:815782/1‑149 (MQ=255)                                                                                                                                        aaTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTaa  <  2:344703/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                             TATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAG‑TAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAA  >  NC_000913_3_hsa_tpiA/2106599‑2106881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑