Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,197,502	C→T	G11R (GGA→AGA)	ymfD ←	e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,197,502	0	C	T	100.0%	105.0 / NA	33	G11R (GGA→AGA)	ymfD	e14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/15);  total (18/15)

ACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCA  >  NC_000913_3_hsa_tpiA/1197356‑1197638                                                                                                                                                   |                                                                                                                                         aCTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGAGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                                                                                                        <  2:740106/149‑1 (MQ=255)           tcatcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTTGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATtata                                                                                                                              <  2:77160/149‑1 (MQ=255)             atcaGAATATCTAAGTTTTCCGCAGCCAAAATCACGGGCGTGGCCATTTTTTTTTTTGTTTCTAATATACTCGCGAAGATATCTAGAGGGCATCGTATGGGGTTATGCTGGATTCTCTGTGCGAATATTAACTCTGTGCTtattttttt                                                                                                                            >  2:898552/1‑144 (MQ=255)              tcaGAATATTGAAGTTTTCCGCAGCCAAAAGCAAGGGCGTGGCCATTTTTGTAAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGtt                                                                                                                           <  2:306220/149‑1 (MQ=255)               caGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTc                                                                                                                          >  1:797188/1‑149 (MQ=255)                       cTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAg                                                                                                                  >  1:405833/1‑149 (MQ=255)                        tAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGt                                                                                                                 >  2:568408/1‑149 (MQ=255)                        tAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGt                                                                                                                 <  1:568408/149‑1 (MQ=255)                          aGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTAc                                                                                                               <  1:621230/149‑1 (MQ=255)                            ttttCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTATGTGAATATTATAGTTCAAAGCAAGTACCa                                                                                                             >  1:701439/1‑149 (MQ=255)                              ttCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTGTCAATGCTTCTAATATACTCTCAAAGATCTCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCAtt                                                                                                           <  1:1009409/149‑1 (MQ=255)                                 cGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTTAGCGAATATTAACTCTGTGCATATTATAGTTCACAGCAAGTACCATTCaa                                                                                                        >  2:107714/1‑149 (MQ=255)                                 cGCAGCCAAAATCAAGGGCGTGGCCATTTTCCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa                                                                                                        <  1:168737/149‑1 (MQ=255)                                               aGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTCTGAGGTTTTGCTGCATTCTCTGACCGAATATTAACTCTCTCCATAGTATGGTTCAAAGCAAGTACCATTCAATTAATTTTAttttt                                                                                          >  2:239434/1‑149 (MQ=255)                                                        ccATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGaaaaa                                                                                 >  1:919158/1‑149 (MQ=255)                                                           tttttCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAATGAAAAATTc                                                                              >  1:418886/1‑149 (MQ=255)                                                                   aaTGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttttattt                                                                      >  1:248055/1‑149 (MQ=255)                                                                       cTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATc                                                                  >  1:602216/1‑149 (MQ=255)                                                                                 aCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGAtt                                                        <  1:983608/149‑1 (MQ=255)                                                                                 aCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGAtt                                                        >  1:522447/1‑149 (MQ=255)                                                                                                tAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTACTCTTACTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCgacttgactt                                         <  1:893144/149‑1 (MQ=255)                                                                                                      gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGtt                                   <  1:368486/149‑1 (MQ=255)                                                                                                       cATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCaa                                                                                                                   >  1:549135/1‑68 (MQ=255)                                                                                                       cATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCaa                                                                                                                   <  2:549135/68‑1 (MQ=255)                                                                                                        aTCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCt                                 >  2:281082/1‑149 (MQ=255)                                                                                                                tggTTTTGCTGCATTCTCTGAGTGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTAGCATTCAATTAATGTTTTTTTTAGTGATAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTaa                         <  2:243093/148‑1 (MQ=255)                                                                                                                aGGTTTTGTTGCATTCGCTGAGCGAATATTAACTCTGTGGATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGGTTTTTCCGACTTGACTTTCAGTTCTGGGCTTaa                         <  2:418886/149‑1 (MQ=255)                                                                                                                aGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTTTGCATATTATAGTTTAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTaa                         <  1:261205/149‑1 (MQ=255)                                                                                                                        ctgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaa                 >  1:796073/1‑149 (MQ=255)                                                                                                                         tgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaag                >  1:617804/1‑149 (MQ=255)                                                                                                                            aTTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaa             >  1:184193/1‑149 (MQ=255)                                                                                                                                      cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATAcc   >  1:402317/1‑149 (MQ=255)                                                                                                                                       gAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTCGACTTTCTTTGCTGGGCTTAACTGTCAAAGCAAAGATAATACca  <  1:558874/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                         ACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCA  >  NC_000913_3_hsa_tpiA/1197356‑1197638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑