Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,111,506	T→G	intergenic (+821/+91)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,111,506	0	T	G	100.0%	271.4 / NA	76	intergenic (+821/+91)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/37);  total (39/37)

GAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTC  >  NC_000913_3_hsa_tpiA/4111360‑4111647                                                                                                                                                   |                                                                                                                                              gAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTgata                                                                                                                                            >  4:1668/1‑150 (MQ=255)    cacaAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGc                                                                                                                                         <  2:651813/150‑1 (MQ=255)       aaaCCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGaa                                                                                                                                      <  1:840719/150‑1 (MQ=255)        aaCCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAAt                                                                                                                                     >  2:226966/1‑150 (MQ=255)           caccTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATaaa                                                                                                                                  <  1:226966/150‑1 (MQ=255)                   tgtCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATg                                                                                                                          >  3:257634/1‑150 (MQ=255)                     tCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgc                                                                                                                        >  1:84706/1‑150 (MQ=255)                        gccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgccgc                                                                                                                     >  4:292949/1‑150 (MQ=255)                                 ttGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                            <  1:207555/150‑1 (MQ=255)                                          ttCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCGTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTc                                                                                                   <  1:481514/150‑1 (MQ=255)                                          ttCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTc                                                                                                   >  4:213031/1‑150 (MQ=255)                                          ttCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTc                                                                                                   >  1:723018/1‑150 (MQ=255)                                          ttCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTc                                                                                                   >  4:124166/1‑150 (MQ=255)                                          ttCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGCTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTc                                                                                                   >  4:218474/1‑150 (MQ=255)                                           tCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCt                                                                                                  <  3:1668/150‑1 (MQ=255)                                            cAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgcc                                                                                                                       <  1:364019/128‑1 (MQ=255)                                            cAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgcc                                                                                                                       >  2:364019/1‑128 (MQ=255)                                             agagTGCCGATGAGCTCCCCCAGACACTGCTTCCGCCCGTTCATCTGCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAGGGAAGATGGCCGCCCCGCAGGGCAGCGGGTCtgt                                                                                                >  2:445246/1‑150 (MQ=255)                                                   ccGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                                          <  4:601785/150‑1 (MQ=255)                                                           cTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                                                  >  4:367040/1‑150 (MQ=255)                                                           cTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                                                  >  4:162474/1‑150 (MQ=255)                                                               cccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTc                                                                              >  3:94674/1‑150 (MQ=255)                                                               cccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTc                                                                              >  1:680005/1‑150 (MQ=255)                                                                 cAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCAt                                                                            >  4:2305/1‑150 (MQ=255)                                                                  aGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATc                                                                           <  3:2305/150‑1 (MQ=255)                                                                    aCTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCgg                                                                         <  2:929177/150‑1 (MQ=255)                                                                          cTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                                          >  4:255560/1‑127 (MQ=255)                                                                          cTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                                          <  3:255560/127‑1 (MQ=255)                                                                          cTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaaa                                                                   <  4:187828/150‑1 (MQ=255)                                                                           ttCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCt                                                                                                  >  3:225667/1‑118 (MQ=255)                                                                           ttCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCt                                                                                                  <  4:225667/118‑1 (MQ=255)                                                                                    ttCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgctt                                                         >  1:640110/1‑150 (MQ=255)                                                                                     tCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagca                                                                                                       >  2:22935/1‑103 (MQ=255)                                                                                     tCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagca                                                                                                       <  1:22935/103‑1 (MQ=255)                                                                                     tCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgcttt                                                        <  2:84706/150‑1 (MQ=255)                                                                                       aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                                      <  3:367040/150‑1 (MQ=255)                                                                                          ttCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCAt                                                                            >  2:487679/1‑125 (MQ=255)                                                                                          ttCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCAt                                                                            <  1:487679/125‑1 (MQ=255)                                                                                           tCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGGAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                                                  <  3:439972/118‑1 (MQ=255)                                                                                           tCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGGAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                                                  >  4:439972/1‑118 (MQ=255)                                                                                           tCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATaga                                                                                   <  3:5256/117‑1 (MQ=255)                                                                                           tCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATaga                                                                                   >  4:5256/1‑117 (MQ=255)                                                                                            ccAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACATAGGCTTTGCTTTTTGTCAt                                                 >  2:994760/1‑150 (MQ=255)                                                                                             cAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAtt                                                <  2:723018/150‑1 (MQ=255)                                                                                             cAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAtt                                                <  3:213031/150‑1 (MQ=255)                                                                                                tttCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTAt                                             <  2:640110/150‑1 (MQ=255)                                                                                                     cccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttg                                                            <  1:525313/130‑1 (MQ=255)                                                                                                     cccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttg                                                            >  2:525313/1‑130 (MQ=255)                                                                                                     cccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaaa                                        >  2:706845/1‑150 (MQ=255)                                                                                                      ccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGta                                                                                       >  2:764790/1‑102 (MQ=255)                                                                                                      ccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGta                                                                                       <  1:764790/102‑1 (MQ=255)                                                                                                        aaCGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCt                                     >  3:208341/1‑150 (MQ=255)                                                                                                           gaagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCa                                  >  2:575991/1‑150 (MQ=255)                                                                                                                aCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGa                             <  4:257634/150‑1 (MQ=255)                                                                                                                 cTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGCCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAt                            >  4:373808/1‑150 (MQ=255)                                                                                                                  ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGTCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAtt                           >  4:328836/1‑150 (MQ=255)                                                                                                                    ccTGGAGGGCGCCATTTTCATGCTCCACGCTGATACGCGAATAAGGGAAGATGGCAGTCCCGTAGGGCGGGGGGTTTGTGGAGCTGTATAGAGACTCATCCGGTCAAAGTTTTTGCTTTTTGTCAGTGATTCGAACCGTCAAGCGACTCa                         <  2:92145/150‑1 (MQ=255)                                                                                                                    ccTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTTTTTTTTTTTCAAACCTTCAAGCGATTCa                         >  2:190073/1‑150 (MQ=255)                                                                                                                    ccTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCa                         >  4:181343/1‑150 (MQ=255)                                                                                                                      tGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGcac                                                                      <  4:67525/103‑1 (MQ=255)                                                                                                                      tGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGcac                                                                      >  3:67525/1‑103 (MQ=255)                                                                                                                      tGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGa                       <  3:439045/150‑1 (MQ=255)                                                                                                                       ggAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAt                      <  3:124166/150‑1 (MQ=255)                                                                                                                       ggAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAt                      >  1:713572/1‑150 (MQ=255)                                                                                                                           ggCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGta                                                                                       <  3:21098/81‑1 (MQ=255)                                                                                                                           ggCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGta                                                                                       >  4:21098/1‑81 (MQ=255)                                                                                                                                cATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                            <  2:422047/55‑1 (MQ=255)                                                                                                                                cATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                            >  1:422047/1‑55 (MQ=255)                                                                                                                                  ttttAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCtt           >  1:278644/1‑150 (MQ=255)                                                                                                                                   tttAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTa          >  2:705430/1‑150 (MQ=255)                                                                                                                                      aaTTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATc       <  1:575991/150‑1 (MQ=255)                                                                                                                                        ttCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCgg     >  1:882786/1‑150 (MQ=255)                                                                                                                                         tctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGt    <  2:882786/150‑1 (MQ=255)                                                                                                                                         tctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGt    <  1:713342/150‑1 (MQ=255)                                                                                                                                          ctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGtt   <  1:445246/150‑1 (MQ=255)                                                                                                                                           tcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGTCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTc  <  3:328836/150‑1 (MQ=255)                                                                                                                                           gccACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTc  <  1:705430/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                              GAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTC  >  NC_000913_3_hsa_tpiA/4111360‑4111647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑