Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,158,965 T→G M165R (ATG→AGG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,158,9650TG100.0% 248.3 / NA 69M165R (ATG→AGG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/33);  total (36/33)

TTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGG  >  NC_000913_3_hsa_tpiA/4158821‑4159108
                                                                                                                                                |                                                                                                                                               
ttCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGc                                                                                                                                            >  1:158999/1‑150 (MQ=255)
   cAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTgg                                                                                                                                         <  2:910356/150‑1 (MQ=255)
       gcgggAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGctat                                                                                                                                     <  2:971321/147‑1 (MQ=255)
            aaaTGTATCGGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTTGACAGCGGCAAACTCGATGGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATg                                                                                                                                <  2:595243/150‑1 (MQ=255)
                   tCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCa                                                                                                                         >  1:383817/1‑150 (MQ=255)
                      gCATGAAGCACAGACCCACCAGTTACTGGCGCATCTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTg                                                                                                                                          <  2:628578/130‑1 (MQ=255)
                      gCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTg                                                                                                                                          >  1:628578/1‑130 (MQ=255)
                               acaGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGaa                                                                                                             <  2:158999/150‑1 (MQ=255)
                                 aGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAAcc                                                                                                           >  2:818507/1‑150 (MQ=255)
                                   aCCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACcgc                                                                                                         >  3:272364/1‑150 (MQ=255)
                                     ccaccaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGa                                                                                                       >  1:628646/1‑150 (MQ=255)
                                       accaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGaa                                                                                                                              >  2:344452/1‑125 (MQ=255)
                                       accaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGaa                                                                                                                              <  1:344452/125‑1 (MQ=255)
                                         caGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGc                                                                                                   <  1:473464/150‑1 (MQ=255)
                                                  gcgcAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATg                                                                                          >  2:417787/1‑150 (MQ=255)
                                                           gACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTATTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTg                                                                                 <  2:750936/150‑1 (MQ=255)
                                                                  gCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGt                                                                                                                                               <  1:262528/81‑1 (MQ=255)
                                                                          gATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAActg                                                                  <  2:628646/150‑1 (MQ=255)
                                                                               cGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGaa                                                                                                      >  2:594458/1‑109 (MQ=255)
                                                                               cGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGaa                                                                                                      <  1:594458/109‑1 (MQ=255)
                                                                                 tGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTg                                                                                                                                          <  1:65155/71‑1 (MQ=255)
                                                                                 tGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTg                                                                                                                                          >  2:65155/1‑71 (MQ=255)
                                                                                   aTCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTg                                                         <  3:42526/150‑1 (MQ=255)
                                                                                      cTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGaa                                                      >  4:134403/1‑150 (MQ=255)
                                                                                          cgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATg                                                            >  3:351130/1‑140 (MQ=255)
                                                                                          cgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATg                                                            <  4:351130/140‑1 (MQ=255)
                                                                                          cgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATg                                                  <  2:383817/150‑1 (MQ=255)
                                                                                             tGGTGAAAGAGAGCGAAGCATTCATTGAGGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTc                                               >  1:405860/1‑150 (MQ=255)
                                                                                             tGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTc                                               >  1:411861/1‑150 (MQ=255)
                                                                                               gTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAActgc                                                                 >  3:46864/1‑130 (MQ=255)
                                                                                               gTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAActgc                                                                 <  4:46864/130‑1 (MQ=255)
                                                                                                tGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGaa                                                                                                                              <  1:198317/68‑1 (MQ=255)
                                                                                                tGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGaa                                                                                                                              >  2:198317/1‑68 (MQ=255)
                                                                                                tGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCATGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaa                                                                       >  4:445663/1‑123 (MQ=255)
                                                                                                tGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCATGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaa                                                                       <  3:445663/123‑1 (MQ=255)
                                                                                                    agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTggg                                                                                                                 >  4:367460/1‑77 (MQ=255)
                                                                                                    agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTggg                                                                                                                 <  3:367460/77‑1 (MQ=255)
                                                                                                       gagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                     >  2:714187/1‑150 (MQ=255)
                                                                                                       gagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                     >  1:48745/1‑150 (MQ=255)
                                                                                                          cGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCa                                                                                                                         <  2:560154/63‑1 (MQ=255)
                                                                                                          cGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCa                                                                                                                         >  1:560154/1‑63 (MQ=255)
                                                                                                          cGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTg                                                         <  4:120333/127‑1 (MQ=255)
                                                                                                          cGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTg                                                         >  3:120333/1‑127 (MQ=255)
                                                                                                            aaGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc                                >  2:407603/1‑150 (MQ=255)
                                                                                                            aaGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc                                >  2:913661/1‑150 (MQ=255)
                                                                                                                    attGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGa                                                                                            <  2:335850/82‑1 (MQ=255)
                                                                                                                    attGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGa                                                                                            >  1:335850/1‑82 (MQ=255)
                                                                                                                    attGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATg                        >  1:941999/1‑150 (MQ=255)
                                                                                                                    atcgAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATg                        >  2:719848/4‑150 (MQ=255)
                                                                                                                             ccGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGt               >  1:202853/1‑150 (MQ=255)
                                                                                                                             ccGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATATGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGt               <  4:315031/150‑1 (MQ=255)
                                                                                                                              cGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt              <  4:272364/150‑1 (MQ=255)
                                                                                                                              cGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt              <  2:202853/150‑1 (MQ=255)
                                                                                                                                   tttGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTc                                               <  4:578853/112‑1 (MQ=255)
                                                                                                                                   tttGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTc                                               >  3:578853/1‑112 (MQ=255)
                                                                                                                                   tttGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa         >  2:852274/1‑150 (MQ=255)
                                                                                                                                   tttGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTAGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa         <  1:907308/150‑1 (MQ=255)
                                                                                                                                     tgatgaGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGc       >  3:335090/1‑150 (MQ=255)
                                                                                                                                      gatgaGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                   <  2:334179/137‑1 (MQ=255)
                                                                                                                                      gatgaGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                   >  1:334179/1‑137 (MQ=255)
                                                                                                                                       atgaGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg     <  2:405860/150‑1 (MQ=255)
                                                                                                                                        tgaGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgg    <  1:913661/150‑1 (MQ=255)
                                                                                                                                         gaGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                     <  3:578342/116‑1 (MQ=255)
                                                                                                                                         gaGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                     >  4:578342/1‑116 (MQ=255)
                                                                                                                                          aGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg  >  3:79245/1‑150 (MQ=255)
                                                                                                                                          aGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg  <  1:852274/150‑1 (MQ=255)
                                                                                                                                            ccAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCAc                                             >  4:29461/1‑105 (MQ=255)
                                                                                                                                            ccAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCAc                                             <  3:29461/105‑1 (MQ=255)
                                                                                                                                             cAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATg                                                  >  3:579835/1‑99 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGG  >  NC_000913_3_hsa_tpiA/4158821‑4159108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑