Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 1,158,559 2 bp→TC coding (691‑692/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,158,5590CT98.9% 300.5 / NA 87Q231* (CAG→TAG) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (48/38);  total (49/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913_3_hsa_tpiA1,158,5600AC98.9% 297.3 / ‑3.8 87coding (692/1434 nt)ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (48/38);  minor base . (1/0);  total (49/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCG  >  NC_000913_3_hsa_tpiA/1158417‑1158702
                                                                                                                                               ||                                                                                                                                              
tcAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTTGTCTGCACCACAGCTGGAACGTACCATTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGtttt                                                                                                                                          >  3:181844/1‑150 (MQ=255)
    tGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCAc                                                                                                                                      >  3:619142/1‑150 (MQ=255)
       gctgctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGc                                                                                                                                   <  1:679619/150‑1 (MQ=255)
           ctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACa                                                                                                                               >  1:552486/1‑150 (MQ=255)
               ccAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTcc                                                                                                                           <  2:552486/150‑1 (MQ=255)
                cAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACGGTTTGGTTTGCACCAGATCTGAAACGTACATTTCCAGATGCAGTTTGGTGAATACACCAAGGAAGCAGTGTC‑GGTTTTCTACGGCGAGAtccga                                                                                                                          >  4:575859/1‑146 (MQ=255)
                 aGAACCCGTTATTTGCGGTTGGCAGTGACGGGTCGAGTGAACTTCGGCTATTAGCGGTTGGTGTTCACGACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCcgc                                                                                                                         <  1:601094/150‑1 (MQ=255)
                 aGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACgg                                                                                                                                    >  2:992876/1‑139 (MQ=255)
                 aGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACgg                                                                                                                                    <  1:992876/139‑1 (MQ=255)
                    aCCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAGCGCAGCAG‑GTTCGGTTTTCAACGGCGACATTCCGAGtt                                                                                                                      <  2:399315/150‑1 (MQ=255)
                      ccGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTtat                                                                                                                    >  2:732456/1‑150 (MQ=255)
                       cGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTtata                                                                                                                   >  4:384564/1‑150 (MQ=255)
                                      gCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGa                                                                                                    >  1:203826/1‑150 (MQ=255)
                                        aTTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCg                                                                                                              <  2:147684/138‑1 (MQ=255)
                                        aTTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCg                                                                                                              >  1:147684/1‑138 (MQ=255)
                                        aTTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACt                                                                                                  >  2:685594/1‑150 (MQ=255)
                                         tttACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTg                                                                                                 >  3:502094/1‑150 (MQ=255)
                                            aCGGTTTCATCGAACGTTGCCTGGTACCGTTTGTTTTGTATTACATCTGGACCGTACCTTTCCAGATGCACATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGTAGGGTGTCCCGACTGCgg                                                                                              <  2:398783/150‑1 (MQ=255)
                                            aCGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                              <  4:322121/150‑1 (MQ=255)
                                                     tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGt                                                                                     >  1:310715/1‑150 (MQ=255)
                                                     tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGt                                                                                     >  1:847046/1‑150 (MQ=255)
                                                       gAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCt                                                                                   >  1:652019/1‑150 (MQ=255)
                                                           gTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggtg                                                                               <  3:388532/150‑1 (MQ=255)
                                                                 tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTg                                                                                                          <  2:2071/117‑1 (MQ=255)
                                                                 tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTg                                                                                                          >  1:2071/1‑117 (MQ=255)
                                                                 tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTa                                                                                           <  3:444642/132‑1 (MQ=255)
                                                                 tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTa                                                                                           >  4:444642/1‑132 (MQ=255)
                                                                 tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTcc                                                                         >  2:385960/1‑150 (MQ=255)
                                                                      ccGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTc                                                                    >  1:163707/1‑150 (MQ=255)
                                                                      ccGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTc                                                                    <  4:619142/150‑1 (MQ=255)
                                                                       cGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCGTCCTGTTCa                                                                   <  2:203826/150‑1 (MQ=255)
                                                                         tttGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggt                                                                                <  2:931686/135‑1 (MQ=255)
                                                                         tttGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggt                                                                                >  1:931686/1‑135 (MQ=255)
                                                                              tCTGCACCACATCTGGCACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTa                                                            >  2:786091/1‑150 (MQ=255)
                                                                              tCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTa                                                            >  2:628709/1‑150 (MQ=255)
                                                                              tCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTa                                                            >  1:127222/1‑150 (MQ=255)
                                                                                  caccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGt                                                        >  2:547562/1‑150 (MQ=255)
                                                                                      acaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGc                                                    >  4:189507/1‑150 (MQ=255)
                                                                                      acaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAAGACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGc                                                    <  2:775248/150‑1 (MQ=255)
                                                                                           tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaaaa                                                                <  2:166095/133‑1 (MQ=255)
                                                                                           tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaaaa                                                                >  1:166095/1‑133 (MQ=255)
                                                                                           tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCt                                               >  1:375060/1‑150 (MQ=255)
                                                                                              aaCGTACCTTTCCAGATGCAGACTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCg                                                                                                              <  4:373647/84‑1 (MQ=255)
                                                                                              aaCGTACCTTTCCAGATGCAGACTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCg                                                                                                              >  3:373647/1‑84 (MQ=255)
                                                                                               aCGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCg                                           <  1:685594/150‑1 (MQ=255)
                                                                                                 gTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCa                                         >  1:646815/1‑150 (MQ=255)
                                                                                                   aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                              >  1:904921/1‑95 (MQ=255)
                                                                                                   aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                              <  2:904921/95‑1 (MQ=255)
                                                                                                   aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACTGTCTGc                                                    <  2:554915/137‑1 (MQ=255)
                                                                                                   aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGc                                                    >  1:554915/1‑137 (MQ=255)
                                                                                                     cTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                     >  3:200690/1‑150 (MQ=255)
                                                                                                      tttCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGATGCCGCAATTGc                                    >  4:501323/1‑150 (MQ=255)
                                                                                                       ttCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCt                                   <  1:732456/150‑1 (MQ=255)
                                                                                                          cAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTAt                                 <  1:421200/149‑1 (MQ=255)
                                                                                                          cAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTAt                                 >  2:421200/1‑149 (MQ=255)
                                                                                                           aGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCa                                                  <  1:146156/131‑1 (MQ=255)
                                                                                                           aGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCa                                                  >  2:146156/1‑131 (MQ=255)
                                                                                                            gATGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTg                              <  3:399554/150‑1 (MQ=255)
                                                                                                             aTGCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTgg                             <  2:310715/150‑1 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGtt                                                                                                                      >  3:298598/1‑59 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGtt                                                                                                                      <  4:298598/59‑1 (MQ=255)
                                                                                                                 aGATTGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCAct                         <  1:786091/150‑1 (MQ=255)
                                                                                                                 aGATGGGTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACGCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCAct                         <  2:685680/150‑1 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGt                                                                                            >  3:381015/1‑77 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGt                                                                                            <  4:381015/77‑1 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAt                                       <  1:437719/130‑1 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAt                                       >  2:437719/1‑130 (MQ=255)
                                                                                                                         gAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTaaa                 >  3:289186/1‑150 (MQ=255)
                                                                                                                         gAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTaaa                 <  1:445465/150‑1 (MQ=255)
                                                                                                                         gAATACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTaaa                 >  2:42130/1‑150 (MQ=255)
                                                                                                                          aaTACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAc                <  3:501323/150‑1 (MQ=255)
                                                                                                                          aaTACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAc                >  1:278397/1‑150 (MQ=255)
                                                                                                                          aaTACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCGAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAc                >  1:278456/1‑150 (MQ=255)
                                                                                                                           aTACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAcc               >  2:973794/1‑150 (MQ=255)
                                                                                                                           aTACACCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAcc               <  2:847046/150‑1 (MQ=255)
                                                                                                                              cacCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGa            <  2:127222/150‑1 (MQ=255)
                                                                                                                               acCAACGCAGCTG‑GTTCGGTTTTCGCCTGCCACAGGCCACGCGATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGCCTGGCATCTGCCGCGATTGCTATCTGGCACTCTGCTAAACCAGaa           >  2:1012039/1‑150 (MQ=255)
                                                                                                                               acCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTgg                             >  3:615725/1‑132 (MQ=255)
                                                                                                                               acCAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTgg                             <  4:615725/132‑1 (MQ=255)
                                                                                                                                ccAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGCCCCGACTGCGGGTAAACTGTCTGGGGGCTTCCTTTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaaa          >  3:437588/1‑150 (MQ=255)
                                                                                                                                 cAACGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaaaa         >  4:116233/1‑150 (MQ=255)
                                                                                                                                  aaCGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAAc        <  2:375060/150‑1 (MQ=255)
                                                                                                                                  aaCGCAGCAG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAAc        >  1:891590/1‑150 (MQ=255)
                                                                                                                                     gcagcaG‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgc     <  4:181844/150‑1 (MQ=255)
                                                                                                                                      cagcag‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcg    <  2:652019/150‑1 (MQ=255)
                                                                                                                                       agcag‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcgc   >  1:1010968/1‑150 (MQ=255)
                                                                                                                                        gcag‑GTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcgcg  >  2:796679/1‑150 (MQ=255)
                                                                                                                                               ||                                                                                                                                              
TCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAG‑GTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCG  >  NC_000913_3_hsa_tpiA/1158417‑1158702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑