New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	4615228 =	46 (1.250)	3 (0.090)	3/262	NT	6.5%	coding (298/864 nt)	yjjW	putative pyruvate formate lyase activating enzyme
	?	NC_000913_3_hsa_tpiA	4615258 =	43 (1.220)					coding (268/864 nt)	yjjW	putative pyruvate formate lyase activating enzyme

TTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCC  >  NC_000913_3_hsa_tpiA/4615110‑4615401                                                                                                                                                     |                                                                                                                                                tttttGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTGGTCGGACATGGCTACGCACTTCGTCCACGCTCATGGCTTGCGCCATGGGCGTGGc                                                                                                                                                 >  1:403976/1‑149 (MQ=255)      gATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGtt                                                                                                                                            >  1:346089/1‑149 (MQ=255)       aTAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGATCATGGAGTGCGCCATGGGCGTGGCctgctg                                                                                                                                           >  2:551911/1‑149 (MQ=255)           cAGCAAACAGCACCACCACACATGGCAGCTGGGGCTTGGCTTCACGGTCACTCACCGTTATCCCTTCGATAAACAGGACTGCTTTGCGGACAGGGCTAAGCACTTCGTCCACGCTCATTGATTGCGCCATGGGCGTGGCATGTTGCGGa                                                                                                                                       <  2:395774/149‑1 (MQ=255)                 aCAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACctc                                                                                                                                 >  1:273636/1‑149 (MQ=255)                     cgcCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTca                                                                                                                             <  1:671232/149‑1 (MQ=255)                      gcCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTcag                                                                                                                            >  1:534094/1‑149 (MQ=255)                       ccaccaCAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTcagg                                                                                                                           >  1:312051/1‑149 (MQ=255)                        caccacAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCAACGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTcaggc                                                                                                                          <  2:451066/149‑1 (MQ=255)                          ccacAAACGGCAGCTGGGTCGTGGCTTCACGGCCACTCACAGTTAACACTTCGACAAACAGAACTGCTTTGAGAACATAGCTAGGAAATTCGTCCACGATCGTAGTTTGTGCATTGGGCTGGGCATGTTGCGGGCACATCTTcaggcag                                                                                                                        >  2:484121/1‑149 (MQ=255)                             caAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTa                                                                                                                     <  1:674826/149‑1 (MQ=255)                                aCGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATca                                                                                                                  <  1:38124/149‑1 (MQ=255)                                  ggCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATcaca                                                                                                                <  2:694989/149‑1 (MQ=255)                                       gattgttgacggcaaagtggtgtggaacgctgtggtttgcgagcagtgtgatacctgcctgaagaggtgtccgcaacatgCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTg                                                                                                                                           <  2:635457‑M1/37‑1 (MQ=255)                                       gattgttgacggcaaagtggtgtggaacgctgtggtttgcgagcagtgtgatacctgcctgaagaggtgtccgcaacatgCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTg                                                                                                                                           >  1:635457‑M1/81‑117 (MQ=255)                                       cTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCAGGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTCCt                                                                                                           <  2:55424/149‑1 (MQ=255)                                             cctGGCTTCACCGCCACTCACCGGTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCaaa                                                                                                     <  2:572112/147‑1 (MQ=255)                                              gTGGCTTCAACGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAAc                                                                                                    <  1:60083/149‑1 (MQ=255)                                               tGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGTCAAGCACGTCGTCCACGCTCATAGATTGCGCCATGGGCGTGGCATGTTGCGCACAACTCTTCCGGCCAGTATCACACCGCCCACaacca                                                                                                   >  2:332320/1‑146 (MQ=255)                                                ggCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACca                                                                                                  <  1:212146/149‑1 (MQ=255)                                                  cTTCACCGGCACTACCGGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACcaca                                                                                                <  2:527391/149‑1 (MQ=255)                                                   ttCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAg                                                                                               >  2:314751/1‑149 (MQ=255)                                                     cACCGGCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCg                                                                                             <  2:632085/149‑1 (MQ=255)                                                      aCCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGt                                                                                            >  1:662986/1‑149 (MQ=255)                                                             cTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGTGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCCGGTATCACACTGCTCGCAAACCACAGCGTTacacac                                                                                     >  1:5505/1‑149 (MQ=255)                                                             cTCACCGTTATCCCTGCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCacac                                                                                     <  1:314996/149‑1 (MQ=255)                                                                     tATCCCTACGAGAAACAGCCCTCCTTTGCTGACACGCCTAAGCCCTACACCACCCCTTATCGATTACGCCATGGGCATCGCACGTTGCGGACACCTCTTCAGGCAGGTATCACTCTGCTCCCACACCACAGCGTTCCACACCACTTTGc                                                                             <  2:523911/149‑1 (MQ=255)                                                                      aTCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCGCTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATGACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGcc                                                                            >  2:398837/1‑149 (MQ=255)                                                                          cTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACCCCACTTTGCCGTcc                                                                        >  1:680282/1‑148 (MQ=255)                                                                           ttCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTcaa                                                                       <  1:170159/149‑1 (MQ=255)                                                                           ttCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCAAACTGATCGCAAACCCCAGCGTTCCACACCCCCTTCCCgcaca                                                                       >  2:522220/1‑145 (MQ=255)                                                                               aTAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAAt                                                                   <  1:57794/149‑1 (MQ=255)                                                                               aTAAACAGCACCGCGTCGCGGACATGGCTCAGCACTTCGTCCACGCTCACGGATTGCGCCATGGGCGTGGCATGTTGCGGACCCCGCTTCAGGCAGGTATGACACTGCTCGCACACCACAGCGTTCCACACCACACCTCCGTCAACAAt                                                                   >  2:629988/1‑149 (MQ=255)                                                                                  aaCAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTg                                                                <  1:248721/149‑1 (MQ=255)                                                                                         cTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGcc                                                         >  1:622509/1‑149 (MQ=255)                                                                                          tGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCt                                                        <  1:170388/149‑1 (MQ=255)                                                                                          tGCTTTGCGGACATGGCTAAGCACTCCATCGGCGTGCGTGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCAAGCTGCTCGCAACCCACAGCGTTACACACCCCTTTCCCGTCAACAATCTGCCACGccc                                                        >  2:672200/1‑148 (MQ=255)                                                                                                gCGGACCTGGCAATGCATGTCGTCAACGCTCATCAATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGTGTTCCACATCACTTTGCCTTCAACAATCTGCAACGCCTGATGCg                                                  <  2:173574/149‑1 (MQ=255)                                                                                                  ggACCTGGCTAAGCACTTCGTCCGCGCTCAGGGATTGCGCCATGGGAGTGGCATTTTGCGGACACCTCTTCATGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCGCTTTGCCGTCAACAATCTGCAACGCCTGATGCGGa                                                <  2:459063/149‑1 (MQ=255)                                                                                                           tAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGgc                                       <  2:470278/149‑1 (MQ=255)                                                                                                              gCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTTAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGACAACAATCTGCAACGCCTGATGCGGCCACTGTGgcacg                                    >  1:557197/1‑149 (MQ=255)                                                                                                                caacatgccacgcccatggcgcaatccatgagcgtggCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATc                                                                  <  2:635457‑M2/80‑1 (MQ=255)                                                                                                                caacatgccacgcccatggcgcaatccatgagcgtggCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATc                                                                  >  1:635457‑M2/38‑117 (MQ=255)                                                                                                                      tCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTcccc                            <  1:69386/149‑1 (MQ=255)                                                                                                                       ccacgcccatggcgcaatccatgagcgtggCATGTTGCGCACACCTCTTCAGGCACGGATCACACTGCTCGCAAACCCCAGCGTTCCACACCACTTTGCCGTCCCCAATCTCCCACGCCTGATGCGGACCCTGTGGCACGCACtccccc                           >  2:67248‑M2/31‑148 (MQ=255)                                                                                                                        CACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCcac                          <  1:669584‑M1/149‑1 (MQ=255)                                                                                                                        CACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCcac                          >  1:427721‑M1/1‑149 (MQ=255)                                                                                                                         ACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCcaca                         >  1:667389‑M1/1‑149 (MQ=255)                                                                                                                         ACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTACAACGCCTGATGCGGACACTGTAGCACGCACTCCCcaca                         <  1:139231‑M1/149‑1 (MQ=255)                                                                                                                           GCTCATGGCTTGGGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGt                       >  2:156343‑M1/1‑149 (MQ=255)                                                                                                                               aTGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCGGATGCGGACACTGTGGCACGCACTCCCCACAGTCAtt                   <  1:123166/149‑1 (MQ=255)                                                                                                                               aTGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAACCTGCAACACCTGATGCGGCCCCTGTGGCACGCACTCCCCCCAGCCAtt                   >  2:416565/1‑149 (MQ=255)                                                                                                                               aTGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAAGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGACCGCACTCCCCCCAGTCAtt                   >  2:20975/1‑149 (MQ=255)                                                                                                                                    ttGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGGATCAAGCTGCTCGCAAACCACAGCGTTCGACACCACTTTGCCGTCAACAATCTGCAACGCCTGATACGCACACTGTGGCACCCACTCCCCACCTTCTTTGCAAc              >  2:563334/1‑149 (MQ=255)                                                                                                                                            tGGGCCTGGCATGTTGCGGACATCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATc      <  2:191187/149‑1 (MQ=255)                                                                                                                                             gggCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCCTCg     >  2:677120/1‑149 (MQ=255)                                                                                                                                                cgtggCATGTTGCTGACACCTCTTCCGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTcc  <  1:63147‑M2/144‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                TTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCC  >  NC_000913_3_hsa_tpiA/4615110‑4615401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.