Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 1,973,561 G→T 11.4% G588G (GGC→GGA)  cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,973,5610GT11.4% 74.8 / 4.5 36G588G (GGC→GGA) cheAfused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
Reads supporting (aligned to +/- strand):  ref base G (15/16);  new base T (2/2);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

TCAACAATCAGTGCCACGCTGCCGTCGCCAAGAATGGTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCGGCCAGTGGATGGAGATCGGCT  >  NC_000913_3_hsa_tpiA/1973418‑1973708
                                                                                                                                               |                                                                                                                                                   
tCAACAATCAGTGCCACGCTGCCGTCGCCAAGAATGGTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGGGGCGACCGCCACt                                                                                                                                                >  1:221143/1‑149 (MQ=255)
 caacaaTCAGTGCCACGCTGCCGTCGCCAAGAATGGTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCCCCACtt                                                                                                                                               >  2:270304/1‑149 (MQ=255)
  aacaaTCAGTGCCACGCTGCCGTCGCCAAGAATGGTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACttt                                                                                                                                              <  1:119557/149‑1 (MQ=255)
            gctaCGCTGCCGTCGCCAAGGTTGGTCGCAGCAGAATTGCCGGGGACTTTGTGATAGTTACTTTCAAGGTTTCTAACCACATCCTTGTGTTGACCATTTAATTGATCCACCAGCAAGGCGTAGCGGCGCCAGCTACTTTGTAAGATcac                                                                                                                                    <  2:258021/146‑1 (MQ=255)
            gCCCCGCTGCCGTCGCCAAGAATGGTCGCAGCAGAAATGCCGGCGACTATGCGAAAGTTACTTTCAAGCTTTTTAACCACAACCTGGTGTTGACCAATTAATGCATCCCCCAGCAAGGCGTAGCGGCGAACGCCAGTTTGTAAGATcac                                                                                                                                    <  2:258212/149‑1 (MQ=255)
              cACGCTGCCATCGCCAAGAATGGTCGCAGCGGAAATACCGGGGACTTTGCGATAGTTAGTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTAATCCACCAGCCAGGCGACGCGGCGACCGCCACTTTGTTAGATcacca                                                                                                                                  >  2:263958/1‑149 (MQ=255)
                        tCGCCAAGAATGGTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCTCCACTTTGTAAGATCACCACAATTCCCTg                                                                                                                        >  1:342518/1‑149 (MQ=255)
                        tCGCCAAGAATGGTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCTCCACTTTGTAAGATCACCACAATTCCCTg                                                                                                                        >  1:342543/1‑149 (MQ=255)
                                   ggTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAAGTAATTGATCCACCAGCATGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCgg                                                                                                             <  2:99983/149‑1 (MQ=255)
                                                    cGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCTCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACg                                                                                            <  2:342543/149‑1 (MQ=255)
                                                    cGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCTCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACg                                                                                            <  2:342518/149‑1 (MQ=255)
                                                     ggggACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGt                                                                                           <  2:592427/149‑1 (MQ=255)
                                                         aCTTTGCGATAGTTACGTTCAAGGTTTTTAACCACAACCTGGTGTGGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGaa                                                                                       <  1:511330/149‑1 (MQ=255)
                                                           tttGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTCGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTGGCGCCCGCGACGTTGAaca                                                                                     >  2:333222/1‑149 (MQ=255)
                                                                gATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTCATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTc                                                                                <  1:117138/149‑1 (MQ=255)
                                                                        cTTTCTAAGTTTCTAACCAGAACCTGGTTATGACCAATTAATTGCTCCACCAGCACGGCGTAGCGGCAACCGCCACTTTGTAAGATCATAACAATTCCCTGTGTGGCATCGGTTTTCGCGCCCGCGACGTTTAACACTTTCTACAGTTc                                                                        <  2:224617/149‑1 (MQ=255)
                                                                         tttCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTcg                                                                       >  1:359420/1‑149 (MQ=255)
                                                                             aaGGTTTTTAACGACAACCTGGTGTTGACCAATTAATTGATCAACCAGCAAGGCGTCGCGGCGGCCGCCACTTTGTAAGATCACGACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTGCGCAGTGcgacgg                                                                   >  2:406308/1‑148 (MQ=255)
                                                                             aaGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCAAAGTTcgacga                                                                   >  1:553995/1‑149 (MQ=255)
                                                                             aaGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGAGACGTTGAACACTTTCCACAGTTcgacga                                                                   >  2:546324/1‑149 (MQ=255)
                                                                                       aacacaACCTGGTGTTGACCAATTCATTCATCAAACAGCATCGGGGAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGata                                                         <  1:540002/147‑1 (MQ=255)
                                                                                              ccTGGTGTTGACCAATTATTTGCTCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCTCAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCAccc                                                  <  2:284995/149‑1 (MQ=255)
                                                                                                tGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCg                                                >  1:202952/1‑149 (MQ=255)
                                                                                                        gccAATTAAGTGGTCAATCAGCAAGGCGTAGCGGCGCCCGCCACTTTGTAAGTTCCCCACAATTCCCTGGGGGGCTTCGGTTTTCGCACCCGATACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCa                                        <  2:426053/148‑1 (MQ=255)
                                                                                                           aattaattGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCTCAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACATTCCACAGTTCGACGATGGGCAAATATCCACCCCGCATTTCCAACa                                     >  2:88015/1‑149 (MQ=255)
                                                                                                            attcattGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCAc                                    <  1:299495/149‑1 (MQ=255)
                                                                                                             ttaattGATCCACCAGCCAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACAACAATTCCAGGGGTGGCTTCGGTTTTCGCGCCAGCGACGTTGAACACTTTCCCCAGTTCGACGATGGGCTGATATTCCGCCCGCCATTCAAGCAcc                                   >  2:568352/1‑149 (MQ=255)
                                                                                                                       caccaGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTcgacga                                                                   >  2:562800/1‑107 (MQ=255)
                                                                                                                       caccaGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTcgacga                                                                   <  1:562800/107‑1 (MQ=255)
                                                                                                                          caGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCgg                      >  2:487647/1‑149 (MQ=255)
                                                                                                                          caGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCgg                      >  1:382573/1‑149 (MQ=255)
                                                                                                                             cAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCGGCCa                   >  1:200971/1‑149 (MQ=255)
                                                                                                                                         gcgACCGCCACTTTGGAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTCCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCGGCCAGTGGATGGAGAt       <  2:213347/149‑1 (MQ=255)
                                                                                                                                          cgACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCGGCCAGTGGATGGAGATc      <  1:623068/149‑1 (MQ=255)
                                                                                                                                          cgACCGCCACTTGGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGCCGTTGAACACTTTCCACAGTGCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCGGCCAGTGGATGGAGATc      <  1:232903/149‑1 (MQ=255)
                                                                                                                                              cGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGGTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCGGCCAGTGGAAGGAAATCGGCt  >  2:62470/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TCAACAATCAGTGCCACGCTGCCGTCGCCAAGAATGGTCGCAGCAGAAATGCCGGGGACTTTGCGATAGTTACTTTCAAGGTTTTTAACCACAACCTGGTGTTGACCAATTAATTGATCCACCAGCAAGGCGTAGCGGCGACCGCCACTTTGTAAGATCACCACAATTCCCTGGGTGGCTTCGGTTTTCGCGCCCGCGACGTTGAACACTTTCCACAGTTCGACGATGGGCAGATATTCACCCCGCACTTCCAGCACCCGCTCGCCGCCGGCCAGTGGATGGAGATCGGCT  >  NC_000913_3_hsa_tpiA/1973418‑1973708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑