Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,183,136	G→T	69.8%	R637L (CGT→CTT)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,183,136	0	G	T	69.8%	83.0 / 37.4	70	R637L (CGT→CTT)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (11/10);  new base T (29/19);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 6.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAT  >  NC_000913_3_hsa_tpiA/4182990‑4183280                                                                                                                                                   |                                                                                                                                                 gccTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTa                                                                                                                                                <  1:66679/147‑1 (MQ=255) gaCTCCGTATCGTAAAGTGACCGACTGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTa                                                                                                                                                <  2:667026/149‑1 (MQ=255)    tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCa                                                                                                                                             >  1:77583/1‑149 (MQ=255)    tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCAACTTCGTAGAAGACCTGGGCACTTGCCTTAGcc                                                                                                                                             >  1:77171/1‑148 (MQ=255)    tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAAAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCa                                                                                                                                             >  2:418439/1‑149 (MQ=255)    tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATAAAGAAGGCCACTTCGTAGAAGACCTGGTACCTTGCCTTAGCa                                                                                                                                             >  1:149983/1‑149 (MQ=255)    tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACGCCAACTTGGATGAAGAAGGCCACTACGTAGAAGACCTGGTAACTTGCCTTAGCa                                                                                                                                             >  1:598251/1‑149 (MQ=255)        aagcGTAAAGTGACCGACGGTGTTGTAACTGACGAAACTCACCACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAgg                                                                                                                                         <  2:519481/146‑1 (MQ=255)             tAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAGCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCAACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAAt                                                                                                                                    >  1:314264/1‑149 (MQ=255)             tAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGGAACTTGCCTTGGCAAAGGCGAAt                                                                                                                                    >  1:408233/1‑149 (MQ=255)             tAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCCGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGGCACTTTGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAAt                                                                                                                                    >  1:128821/1‑149 (MQ=255)              aaaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATc                                                                                                                                   <  1:105889/149‑1 (MQ=255)                aGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCa                                                                                                                                 >  1:465484/1‑149 (MQ=255)                  tGAGCGATGGGGGTGTAACTGACGAAATTCACGACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGGGAACTCCACCTTCGATGAAGAACGAGATGGCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAACCCAGc                                                                                                                               <  2:115505/149‑1 (MQ=255)                      cGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGt                                                                                                                           >  1:244256/1‑149 (MQ=255)                      cGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGt                                                                                                                           <  1:38303/149‑1 (MQ=255)                        aCGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGACGACCTGGTAACTTGCCTTAGCATAGGCGAATCCAGCTTGTTc                                                                                                                         >  2:200066/1‑149 (MQ=255)                           gtgtTGTAACTGACGGAATCCACTACCTGTCTGCTATCGAAGAAGGCCACTACGGTATCACCCAGGCGAACTCCAACTCGCATCAACAAGCCCACTTCGTCAACGACCTTATAACTTCCCTTAGCAACGCCGAACCCCGCATGCTCacc                                                                                                                      >  2:241841/1‑147 (MQ=255)                                         caagtgcACGACCTCGCTGCTACTGAAGAGGGCATCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                                                                        <  2:27711/143‑1 (MQ=255)                                            aTTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTa                                                                                                     >  1:108335/1‑149 (MQ=255)                                              tCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTCAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGAGTACa                                                                                                   >  1:511069/1‑149 (MQ=255)                                               cACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCCACTTGGATGAAGAAGGCCACTTCTTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTATTCAACCGCGACCAGGTTGACTACAt                                                                                                  >  1:181615/1‑149 (MQ=255)                                                aCTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGACGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATg                                                                                                 >  1:230388/1‑149 (MQ=255)                                                  ttttcgTCTTCTAGCGAGGATGGCAACTACTTTGTCGCCCAGGCGGATGCTAACGTGGATGAAGAAGGCTGGTTCGTAGAAGTGCTGGTGAGTTGTCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGTCCAGGTTGGCTACATggg                                                                                               <  2:300355/144‑2 (MQ=255)                                                  tACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGa                                                                                               <  1:219382/149‑1 (MQ=255)                                                   aCCAGTCTACTATCGAAGAGGCCAACTACGTTATCGAACAGGCGAAAAAGTTTTTGATTTAATGAGGCCAAATCGTAGAATTCCTGGTTAGGGGCCGTAGCAAAGGCGAATCCAGTTTGTTCAGCCGCGACCAGGTTGACGACATGGAc                                                                                              <  2:320389/149‑1 (MQ=255)                                                       gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAAAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTTTTCAGCCGCGACCAGGTTGACTCCATGGACGTAt                                                                                          >  2:168334/1‑149 (MQ=255)                                                          tGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGCCTACATGGACGTATCCa                                                                                       >  1:162851/1‑149 (MQ=255)                                                          tGCTATCGAAGAAGGCAACTACGTTATCACCCCGGAGAGCTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCACGACCAGGTTGACTACATGGACGTATCCa                                                                                       >  2:323504/1‑149 (MQ=255)                                                               tCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGCATCATGATTGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCAACCcag                                                                                  >  1:135751/1‑149 (MQ=255)                                                                  aagaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTCGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCAACCAGGTTGACTACATGGACGTATCCACccaccac                                                                               >  2:32335/1‑148 (MQ=255)                                                                   agaagGCAACTACGTTATCGCCCAGGCGAACTCCCACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCTACCCGGTTAACTACATGGACGTAGGCACCCAGCAgg                                                                              <  2:238506/149‑1 (MQ=255)                                                                     aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAggtg                                                                            >  1:62233/1‑149 (MQ=255)                                                                     aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAggtg                                                                            >  1:138670/1‑149 (MQ=255)                                                                     aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAggtg                                                                            <  2:82055/149‑1 (MQ=255)                                                                     aagGCAACTACGTTATCGCCCAGGCGAACTCAACCCATGGTGACGAAGCCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTAAATGGACGTAACCCCCcaggacggg                                                                            >  2:671407/1‑143 (MQ=255)                                                                          aaCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATc                                                                       <  2:607152/149‑1 (MQ=255)                                                                          aaCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATc                                                                       <  1:556605/149‑1 (MQ=255)                                                                            cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTGCATGGACGTATCCACCAAGCAGGTGGTATCCg                                                                     >  1:137589/1‑149 (MQ=255)                                                                            cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCg                                                                     >  2:588628/1‑149 (MQ=255)                                                                             tACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGt                                                                    >  1:566815/1‑149 (MQ=255)                                                                              aCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCCTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATCGACGTATCCACCCAGCAGGTCGTATCCGTc                                                                   >  2:522302/1‑149 (MQ=255)                                                                                           gccGAACTCGAGCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGa                                                      <  2:621889/147‑1 (MQ=255)                                                                                            gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCAAGCTTGTTCAGCCGCGCCCAGGTTGACTACATGCACGTATCCACCCAGCAGCGCGTATCAGCCCGTGCGTccgtcct                                                     >  2:446676/1‑144 (MQ=255)                                                                                             cGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATc                                                    <  2:330098/149‑1 (MQ=255)                                                                                                 ttCCAACTGGGATGAAGCAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGCGGTATCCGTCCTTGCGTCCCTGATCCCGt                                                <  2:256662/148‑1 (MQ=255)                                                                                                 cttcAACTTGGATGAACGAGGCCACTTCGTAGAAGTCTTGGTAACTTGCCTTAGCAAAGCCGATTCCAGCTTGTTCATCCGCGCCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGACCACGt                                                <  2:643042/146‑1 (MQ=255)                                                                                                 cTCCAACCTGGATGGAGAAGGCCACTTGGTAGAAGATCTGGTAACCTGGTTCAGCAAAGGCGAATCCAGCTTGTCCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGt                                                <  2:90909/149‑1 (MQ=255)                                                                                                  tCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCATGGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAACCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGtt                                               >  1:657922/1‑149 (MQ=255)                                                                                                   ccAACTTGGATGAAGAAGGCCACTTCGAACGCGACCTTTTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCGCGttt                                              <  2:492370/149‑2 (MQ=255)                                                                                                     accTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                            <  1:534321/147‑1 (MQ=255)                                                                                                           gATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCACCCGCGACCAGGTTGACTACATGGACGTATCCACCCACCAGGTGGTATCCGTCGGTGCGCCCCTGATCCCGTTCATgggcac                                      >  2:165047/1‑145 (MQ=255)                                                                                                            gggaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGTACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAacac                                     <  2:519639/147‑1 (MQ=255)                                                                                                            aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAacac                                     >  1:286657/1‑149 (MQ=255)                                                                                                             tGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACg                                    >  1:28883/1‑149 (MQ=255)                                                                                                               aaggagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAt                                  <  1:397678/149‑1 (MQ=255)                                                                                                               aagaagGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAAGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAt                                  <  2:408233/149‑1 (MQ=255)                                                                                                                 gaagGCCACTTCGTAGAAGTCCTGGTCACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGa                                <  2:577476/149‑1 (MQ=255)                                                                                                                 gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGa                                >  1:632302/1‑149 (MQ=255)                                                                                                                 gaagGCCACTTCGTAGAAGACCTGGTAACTTCGGGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGa                                >  2:498739/1‑149 (MQ=255)                                                                                                                   agGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGAGCGTCCCTGATCCCGTTCCTGGAACTCGATGAca                              >  1:617702/1‑148 (MQ=255)                                                                                                                    ggCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGCTCCGCCGCGACCGCGTTTACGACATGCACGTACCCACCCAGCAGGTGGTCGCAGTCGGTGGGTCCCTGAGCCCGATCCTAAAACAACATGActc                             >  2:408939/1‑147 (MQ=255)                                                                                                                      ccACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGCAACACGATGACCCCa                           >  2:230365/1‑149 (MQ=255)                                                                                                                       cACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                          >  1:622833/1‑149 (MQ=255)                                                                                                                            cGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTg                     <  1:665177/149‑1 (MQ=255)                                                                                                                             gTAGAAGACCTGGTAACTTGACGTAGCAAAGGCGCATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGc                    <  2:473674/149‑1 (MQ=255)                                                                                                                               agaagaCCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCAt                  >  1:178679/1‑149 (MQ=255)                                                                                                                               agaagaCCTGGTAACTTGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCAt                  >  2:422938/1‑149 (MQ=255)                                                                                                                                              ttGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa   <  1:574624/149‑1 (MQ=255)                                                                                                                                               tGCCTTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt  <  1:590143/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAT  >  NC_000913_3_hsa_tpiA/4182990‑4183280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑