Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 1,224,239 A→T 11.5% intergenic (+332/+40) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,224,2390AT11.5% 83.3 / 3.5 34intergenic (+332/+40)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (15/14);  new base T (2/2);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTG  >  NC_000913_3_hsa_tpiA/1224091‑1224383
                                                                                                                                                    |                                                                                                                                                
cgcAACCAGGCTTAAACATTTTTGTTTGTGCCTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                                                                                                                                                  <  2:17185/147‑1 (MQ=255)
   aaCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                               >  2:512335/1‑149 (MQ=255)
         gCATAAAGTGTTTGGTGTGGGGGTGGGAACAGCCTAAAGGTGAAGAGGGAGAGGAGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGTCGCTTTACCACAACGTGGATCAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc                                                                                                                                         <  2:300695/149‑1 (MQ=255)
            tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCGGAGATAACTCTGCCtt                                                                                                                                      >  2:232033/1‑149 (MQ=255)
              aagatgttgtgtggtGCGTGGGAAGAGCCTTAAGGTGTAGAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAACCTCAAGGCAGCGTTATCTCTGCCTTGa                                                                                                                                    <  2:352348/143‑1 (MQ=255)
               aCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATCGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCCGAACTAACTCTGCCTTGaa                                                                                                                                   >  2:288028/1‑149 (MQ=255)
                  ttttgttttgTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTGGAAGAt                                                                                                                                >  1:559001/1‑149 (MQ=255)
                       ttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGCTAAATg                                                                                                                           >  1:269407/1‑149 (MQ=255)
                             gctggggAACAGCCTTAGGGTGTAAAGGGGGAGGGGGAAAGAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGTATGAGAGGGATGAAAAACTCAAGGCAGCGATAATTCTGCCTTGAAGCTCAATGCGCttt                                                                                                                     <  2:61778/145‑1 (MQ=255)
                                 gggAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACa                                                                                                                 <  1:309850/149‑1 (MQ=255)
                                   gAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCGGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGc                                                                                                               >  1:205805/1‑149 (MQ=255)
                                    aaCAGCGGTAAGGTGTAAAGGGGGAGGGGGAAATAGCGTTGAGGGGGATCAGCGAGAATATTCGCCGCTTTACCACAACTGGGATGTGAGGGATGAAACACTCAAGGCAGAGATAACTCGGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                                                              <  2:193202/149‑1 (MQ=255)
                                     aCAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg                                                                                                             >  2:198059/1‑149 (MQ=255)
                                          cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                                                                        <  1:210304/149‑1 (MQ=255)
                                          cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                                                                        >  2:382618/1‑149 (MQ=255)
                                           ttAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt                                                                                                       <  1:272728/149‑1 (MQ=255)
                                                gtgtAAAGGGGGAGGTGGAAAGAGCAAGGAGGAGTACCAGCAAGAATTCGCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAg                                                                                                  <  1:213466/149‑1 (MQ=255)
                                                                                  tATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCCCGTTCAGCTCa                                                                >  2:12268/1‑149 (MQ=255)
                                                                                       gCAAGAATACTCGCCGCTTTACGACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt                                                           >  1:280509/1‑149 (MQ=255)
                                                                                         aaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg                                                         >  2:575364/1‑149 (MQ=255)
                                                                                             aTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCAGGCTTATTTCAGCACTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATCGAAat                                                     >  2:582259/1‑149 (MQ=255)
                                                                                                   gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGcc                                               >  1:642593/1‑149 (MQ=255)
                                                                                                    ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGCGCTTATTTCAGCTCATCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCa                                              <  1:581900/149‑1 (MQ=255)
                                                                                                              acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCt                                    >  1:512100/1‑149 (MQ=255)
                                                                                                                  ggTGGTTGACAGGGATGAAAAACGCAAGGCAGACATAACTCTGCCTTGAATATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAATGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAg                                <  2:369438/148‑1 (MQ=255)
                                                                                                                          agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAGGCTCTACgg                        >  2:354399/1‑149 (MQ=255)
                                                                                                                          aaggggATGAAAAACTCAGTGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCATCCGGTAGGGTCTCGGGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACgg                        <  2:239731/146‑1 (MQ=255)
                                                                                                                                        ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCCCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGa          >  1:584372/2‑149 (MQ=255)
                                                                                                                                        cTCAAGGCAGATATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGa          <  1:202055/149‑1 (MQ=255)
                                                                                                                                         tCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTCCGGTTACCATCTCAGGAt         >  1:96708/1‑149 (MQ=255)
                                                                                                                                            aGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa      <  1:408337/149‑1 (MQ=255)
                                                                                                                                             ggCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACAATCTCAGGATCAAt     >  2:111267/1‑149 (MQ=255)
                                                                                                                                             ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAt     <  2:371155/149‑1 (MQ=255)
                                                                                                                                               cAGAGTTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAttt   <  1:281695/149‑1 (MQ=255)
                                                                                                                                               cAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCCGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTATTTTTACCCTCTCAGGATCCAttt   <  2:570746/149‑1 (MQ=255)
                                                                                                                                                agagagAACTATGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTg  <  2:39977/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTG  >  NC_000913_3_hsa_tpiA/1224091‑1224383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑