| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_hsa_tpiA | 1,734,464 | G→C | 20.3% | T10T (ACG→ACC) | mepH → | murein DD‑endopeptidase, space‑maker hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_tpiA | 1,734,464 | 0 | G | C | 20.3% | 42.5 / 5.2 | 24 | T10T (ACG→ACC) | mepH | murein DD‑endopeptidase, space‑maker hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (10/9); new base C (3/2); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
ATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAA > NC_000913_3_hsa_tpiA/1734335‑1734607 | aTAAAATCTATTAACTTTCTCTTCTTTGATGCGTTCGAATGCTCAAGTTTTGTAAATCAGACGCAGGCACGATAGACCTGTCTTTACAGAGGGACGCTTAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTa < 2:315931/149‑1 (MQ=255) aaGCTATTAACTTTCTCTTCTTCTATGAATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTaccac < 2:361343/149‑1 (MQ=255) aaGCTATCAACTTACTCTTCTTCTATGTATGAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTaccac < 2:361177/149‑1 (MQ=255) tATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTg < 1:3688/149‑1 (MQ=255) tATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGAACCCTCAGTAGCGCGGATAAACCGAATTTCGATCACCCTCTGTGCTTTACTTTTTACCCCCCTg > 2:527175/1‑149 (MQ=255) ttttcttcttctATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAAc < 2:648850/146‑1 (MQ=255) tcttcttctATGCATTAGAATCATCAAGTTTTGTAAACCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGcc > 1:363235/1‑149 (MQ=255) tattcttctAAGCATTAGAATCATCAAGTTTTGTAAAACAGAAGCAGGCATGATAGACCTGACTTTACAGATGGACGCTCAGTGGCGCGGATAAAACGTATTTCAATCACGCCCTGTACTTTACTTTTTCCCACCCCTCCTTTAAcccc > 2:230080/3‑149 (MQ=255) ttctATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTCTTTCGATCACGCTCTGTGCTTTACATTTTACCACCCTGCCTTTAACGCCTATgg > 1:567319/1‑149 (MQ=255) tctATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGc > 1:396818/1‑149 (MQ=255) atcatcAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAaag < 1:178517/149‑1 (MQ=255) cAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACCCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTg < 2:118861/149‑1 (MQ=255) gATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCatat < 2:478162/149‑1 (MQ=255) aCCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGAGAGAGTTCTGCTACCACTCATATCACCa > 2:438882/1‑149 (MQ=255) aCCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAAACGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCAATCATATCCCCa > 1:387335/1‑149 (MQ=255) tttACAGAGGGACGCTCAGTGGCGCGGATAAACCGTGTTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGc > 1:388338/1‑149 (MQ=255) acatacggACGCTCAGTGGGGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTAATACCCTGCCTTTATCGTTTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTATCATTCATCTCACCAAAAAAGCAGa < 2:515962/143‑1 (MQ=255) aCAGAGTGACGCTCAGTGGCGCGGATCATCCCTATTTCGATCACCCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGa < 1:168674/149‑1 (MQ=255) aCAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGGTCTGCTACCCCTCATATCACCAACAAAGCAGa > 2:424768/1‑149 (MQ=255) cgcgGATAAACCGTATTTCGATCACCCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAATCAAAAacacacca > 2:657885/1‑145 (MQ=255) gcgGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCaa > 1:208488/1‑149 (MQ=255) tttCGATCCCGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGAcc < 1:370946/149‑1 (MQ=255) tttCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGAGAAAAAGAAAAGCACGGCAACCACCAAGAAGAcc > 1:107812/1‑149 (MQ=255) aTCACCCTCTGTGCTTTACTTTTTACAACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGCGAGTTCTGCTACCACTCATATCACCAAAAAAACAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGaa > 1:626469/1‑149 (MQ=255) | ATAAAAGCTATTAACTTTCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAA > NC_000913_3_hsa_tpiA/1734335‑1734607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |