Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 2,976,684 G→T 19.0% S29I (AGC→ATC)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,976,6840GT19.0% 63.5 / 7.7 36S29I (AGC→ATC) galRgalactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (7/22);  new base T (3/4);  total (10/26)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

AACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGC  >  NC_000913_3_hsa_tpiA/2976537‑2976825
                                                                                                                                                   |                                                                                                                                             
acccAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGc                                                                                                                                              <  2:158952/147‑1 (MQ=255)
   cagcGGGCGTTTTCCGTAACACTGATAGCACGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGaa                                                                                                                                           <  2:511528/146‑1 (MQ=255)
             attCCGGAAACCTGAAAGAAGGTAAGCGTTTACCCACTAAGGTATTTGCATGGCGACCACAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCggc                                                                                                                                 <  2:279764/148‑1 (MQ=255)
                 cGTAACACGTAAAGAATGTAAGCGTTGACGCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCggctggc                                                                                                                             <  1:508949/149‑1 (MQ=255)
                   tAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGgctggctg                                                                                                                           >  1:192242/1‑149 (MQ=255)
                    aaCCCTGAAAGATTGTAAGCGTTTGCGCACTGTGGTATTTGCAGTGGGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCtgt                                                                                                                          <  2:69883/149‑1 (MQ=255)
                        cTGAAAGAATGCAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCAt                                                                                                                      <  1:42673/149‑1 (MQ=255)
                         tGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAACCAGCGAAGCTTCCCGGCTGGCTGTGCATa                                                                                                                     <  2:98388/149‑1 (MQ=255)
                                 tGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGTTGTAGCCCGACTGGCAGGCGTTGGAGGCACCGCTTTTTCCCGCGTCATTAATAAGTCACCCAGAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATg                                                                                                             <  1:255295/149‑1 (MQ=255)
                                   tatgCGTTTACCCACTAGGGTATTTTCAGGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGGTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGa                                                                                                           <  2:66967/146‑1 (MQ=255)
                                     aGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGt                                                                                                         <  1:602240/149‑1 (MQ=255)
                                     aGCGTTTACCACCTAAGGTATTTGCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTTCCGGCTGGCTGTGCATAGTGCAATGGAGt                                                                                                         <  1:171373/149‑1 (MQ=255)
                                      gCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGtc                                                                                                        <  1:462251/149‑1 (MQ=255)
                                              ccACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCt                                                                                                >  2:619103/1‑149 (MQ=255)
                                                                 gcggctgtAAAGGATGTAGCCCGACTGACAGGCTTTGCAGGCTCCACCGTTTCCCGCGTCATTAATAATTCACCCAATGCCAGCGAATCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGaacgccaacgc                                                                             <  2:190751/142‑1 (MQ=255)
                                                                   gACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGACTCGCTGTGCAGCGTGCAACGGGGTCCCTTAGCTATCACCCGcgcgccaacgcac                                                                           >  1:313464/1‑147 (MQ=255)
                                                                    aCCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCCTACCTAGCACCCGAACGCCAACGCCCg                                                                          >  1:51437/1‑149 (MQ=255)
                                                                       aTAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTgc                                                                       <  1:605263/149‑1 (MQ=255)
                                                                                 tAGCGCGACTGGCAGGCGTTGCATTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCgcag                                                             <  1:268360/149‑1 (MQ=255)
                                                                                   ggccGGCTTGCAGGTGGTGCAGGCGCCGCCGTTACGCGCGGAATTAATAAGTCAGCAAGAGCCAGCGCGGCTGCGCGGCTGGCTGTGCAGAGGGCAATGGAGTGTGTTAGCTATCGCCCGAACGCCAACGCCCGTGCGCTGGCgcagca                                                           <  2:568075/147‑1 (MQ=255)
                                                                                              aGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACACCTGaaa                                                >  2:225526/1‑149 (MQ=255)
                                                                                               gtcggttCAGTCGCCACCGTTGCCCGCGTCATTAAGAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGTCTGTGCATAGGTCAATGGAGTCTCTTAGCTATCACCCGAACGCCCACGCCCGTGCGCTGGCGCAGCAGACCACTGAAAc                                               <  2:359788/144‑1 (MQ=255)
                                                                                               ggCGTTGCTCTCTCCACCCTTTCCCGCGTCATTAATAATTCCCCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAAc                                               <  2:265484/149‑1 (MQ=255)
                                                                                                gCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAAcg                                              >  2:267549/1‑149 (MQ=255)
                                                                                                       aGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACggtcggtc                                       <  1:358954/149‑1 (MQ=255)
                                                                                                         tCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTg                                     >  1:380235/1‑149 (MQ=255)
                                                                                                          cGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTgg                                    <  1:553724/149‑1 (MQ=255)
                                                                                                            ccACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCGGCAGACCACTGAAACGGTCGGTCTGGTc                                  >  2:405632/1‑149 (MQ=255)
                                                                                                                cGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTg                              <  1:591320/149‑1 (MQ=255)
                                                                                                                     cccGAGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGAt                         <  2:625069/149‑1 (MQ=255)
                                                                                                                           tCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATCGTGCAATGGAGACTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTcc                   <  1:353122/149‑1 (MQ=255)
                                                                                                                                aataatTCTCCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATcc              <  1:412487/149‑1 (MQ=255)
                                                                                                                                  taatTCACCCACAGCCAGCGAAGCTTCCCGGCCGGCTGTTCATAGTGCAATGGAGTCTCTAAGCTACCACCCGAACCCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGt            <  1:143360/149‑1 (MQ=255)
                                                                                                                                          ccAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGt    >  2:185113/1‑149 (MQ=255)
                                                                                                                                          ccAAAACCAGCGAAGCTCCCCCGCTGGCTGTGCATAGGGCAATGGAGTCTCGTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAATCGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGt    <  2:219076/149‑1 (MQ=255)
                                                                                                                                            aaaGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGGGTGTTAGCTATCCCCCGAACGCCAAGGCACGTGCGCTGGGGCAGCAGACCACTACAACGGTCGGTCTGGGCCGGGGTGAGGGTACCGATCCGCTGTTAGGtag  >  2:238887/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
AACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGC  >  NC_000913_3_hsa_tpiA/2976537‑2976825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑