Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,755,806	C→A	H37N (CAT→AAT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,755,806	0	C	A	100.0%	265.6 / NA	73	H37N (CAT→AAT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/30);  total (43/30)

CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTT  >  NC_000913_3_hsa_tpiA/1755665‑1755946                                                                                                                                              |                                                                                                                                             cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGAc                                                                                                                                      >  1:446950/1‑150 (MQ=255)  gcATCGTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAAAATCGGACCGAAAACGGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACt                                                                                                                                     >  2:984242/2‑150 (MQ=255)   cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTa                                                                                                                                    >  3:119925/1‑150 (MQ=255)        ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAg                                                                                                                               <  2:259653/150‑1 (MQ=255)         tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGa                                                                                                                              >  1:1215158/1‑150 (MQ=255)             aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAacac                                                                                                                          >  2:302347/1‑150 (MQ=255)              aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACg                                                                                                                         <  1:1164019/150‑1 (MQ=255)                ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGAc                                                                                                                                      >  2:659868/1‑135 (MQ=255)                ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGAc                                                                                                                                      <  1:659868/135‑1 (MQ=255)                ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGt                                                                                                                       >  1:1045859/1‑150 (MQ=255)                  aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCa                                                                                                                     >  2:927566/1‑150 (MQ=255)                  aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCa                                                                                                                     >  2:927573/1‑150 (MQ=255)                      actaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAgcgc                                                                                                                 <  4:637838/150‑1 (MQ=255)                        taagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCAt                                                                                                               >  2:402712/1‑150 (MQ=255)                         aagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCAtt                                                                                                              <  4:632278/150‑1 (MQ=255)                          agactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTc                                                                                                             <  1:849776/150‑1 (MQ=255)                          agactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTc                                                                                                             >  2:849776/1‑150 (MQ=255)                                  aTGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTg                                                                                                     <  1:234376/150‑1 (MQ=255)                                    gAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgcg                                                                                                   >  3:508580/1‑150 (MQ=255)                                     aaaaaGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgcgc                                                                                                  >  3:798868/1‑150 (MQ=255)                                      aaaaGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCa                                                                                                 >  1:1310077/1‑150 (MQ=255)                                      aaaaGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCa                                                                                                 >  2:1229020/1‑150 (MQ=255)                                        aaGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAAc                                                                                               <  3:292909/150‑1 (MQ=255)                                            ccAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtga                                                                                           >  1:367511/1‑150 (MQ=255)                                              aaaaTTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatg                                                                                         >  2:1222814/1‑150 (MQ=255)                                                     tttGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGt                                                                                             <  2:1236822/139‑1 (MQ=255)                                                     tttGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGt                                                                                             >  1:1236822/1‑139 (MQ=255)                                                     tttGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa                                                                                  <  1:927566/150‑1 (MQ=255)                                                     tttGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa                                                                                  <  1:927573/150‑1 (MQ=255)                                                        gCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTg                                                                               <  2:1310077/150‑1 (MQ=255)                                                         cACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAAc                                                                                 >  3:159562/1‑147 (MQ=255)                                                         cACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAAc                                                                                 <  4:159562/147‑1 (MQ=255)                                                         cACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGAGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTgg                                                                              >  4:773829/1‑150 (MQ=255)                                                          aCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGt                                                                             >  2:639463/1‑150 (MQ=255)                                                          aCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGt                                                                             <  1:312853/150‑1 (MQ=255)                                                             aTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaaa                                                                          <  3:773829/150‑1 (MQ=255)                                                                  aCCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccgc                                                                     >  3:397622/1‑150 (MQ=255)                                                                      aaaaCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCt                                                                 >  3:461928/1‑150 (MQ=255)                                                                      aaaaCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCt                                                                 >  2:947539/1‑150 (MQ=255)                                                                          ccGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATcc                                                             >  2:326848/1‑150 (MQ=255)                                                                          ccGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATcc                                                             >  4:486103/1‑150 (MQ=255)                                                                             aaTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCa                                                                                                                                >  4:357153/1‑80 (MQ=255)                                                                             aaTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCa                                                                                                                                <  3:357153/80‑1 (MQ=255)                                                                              aTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCt                                                         <  4:397622/150‑1 (MQ=255)                                                                                  gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTg                                                                                                                                        >  4:171415/1‑67 (MQ=255)                                                                                  gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTg                                                                                                                                        <  3:171415/67‑1 (MQ=255)                                                                                  gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGt                                                                                                                       >  3:437203/1‑84 (MQ=255)                                                                                  gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGt                                                                                                                       <  4:437203/84‑1 (MQ=255)                                                                                  gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGAt                                                     >  4:568900/1‑150 (MQ=255)                                                                                  gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGAt                                                     >  4:178001/1‑150 (MQ=255)                                                                                        aTGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATgg                                                                                                                                          >  2:851109/1‑59 (MQ=255)                                                                                        aTGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATgg                                                                                                                                          <  1:851109/59‑1 (MQ=255)                                                                                         tGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAg                                              <  1:1222814/150‑1 (MQ=255)                                                                                             aGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACt                                                                                                                                     <  4:452106/59‑1 (MQ=255)                                                                                             aGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACt                                                                                                                                     >  3:452106/1‑59 (MQ=255)                                                                                             aGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTcc                                          >  1:183529/1‑150 (MQ=255)                                                                                              gCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCg                                         <  2:1045859/150‑1 (MQ=255)                                                                                               cTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCgg                                        <  1:984242/150‑1 (MQ=255)                                                                                                tAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                                       >  1:1198032/1‑150 (MQ=255)                                                                                                 aaaaTGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                                       >  1:1281533/1‑149 (MQ=255)                                                                                                 aaaaTGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                                       <  2:1281533/149‑1 (MQ=255)                                                                                                        tGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCa                               >  3:380188/1‑150 (MQ=255)                                                                                                             gCTGGCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATg                          <  4:119925/150‑1 (MQ=255)                                                                                                                 gCATGAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAAc                      >  1:226862/1‑150 (MQ=255)                                                                                                                     gAACGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGa                  >  1:646105/1‑150 (MQ=255)                                                                                                                      aaCGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGaa                 >  1:810984/1‑150 (MQ=255)                                                                                                                       aCGTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAg                <  1:211049/150‑1 (MQ=255)                                                                                                                         gTTATGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGc              <  2:226862/150‑1 (MQ=255)                                                                                                                            aTGCGTCTGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTCAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGt           <  2:810984/150‑1 (MQ=255)                                                                                                                                   tGAACTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAacgacg    >  1:1083438/1‑150 (MQ=255)                                                                                                                                     aaCTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGaaa                       <  3:727942/129‑1 (MQ=255)                                                                                                                                     aaCTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGaaa                       >  4:727942/1‑129 (MQ=255)                                                                                                                                     aaCTTCTCTAATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGtt  >  3:343172/1‑150 (MQ=255)                                                                                                                                           tctaATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGa                                                      >  1:988322/1‑92 (MQ=255)                                                                                                                                              |                                                                                                                                             CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTT  >  NC_000913_3_hsa_tpiA/1755665‑1755946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑