Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 1,719 T→A N461K (AAT→AAA)  thrA → Bifunctional aspartokinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,7190TA98.8% 285.1 / ‑4.1 80N461K (AAT→AAA) thrABifunctional aspartokinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (36/43);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTG  >  NC_000913_3_hsa_tpiA/1578‑1863
                                                                                                                                             |                                                                                                                                                
gAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCa                                                                                                                                          >  2:1177917/1‑150 (MQ=255)
 aaaTTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAg                                                                                                                                         <  1:1141950/150‑1 (MQ=255)
  aaTTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAgg                                                                                                                                        <  2:291283/150‑1 (MQ=255)
  aaTTCTTTGCCGCACCGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGACCGATCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAgg                                                                                                                                        <  3:813985/150‑1 (MQ=255)
         tGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATc                                                                                                                                           >  2:227789/1‑140 (MQ=255)
         tGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATc                                                                                                                                           <  1:227789/140‑1 (MQ=255)
          gCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTTCTCTGGGTTCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGaa                                                                                                                                <  1:356735/150‑1 (MQ=255)
          gCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGaa                                                                                                                                <  2:736778/150‑1 (MQ=255)
          gCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGaa                                                                                                                                <  2:736779/150‑1 (MQ=255)
           ccGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAg                                                                                                                               >  3:343105/1‑150 (MQ=255)
            cGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCCCTGTCGTGGTAAATAACGATGATGCGACCACTAGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCCGGTTATCGAAgt                                                                                                                              >  2:953951/1‑150 (MQ=255)
              cACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAgtgt                                                                                                                            <  1:704051/150‑1 (MQ=255)
                cTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGttt                                                                                                                          <  4:302018/150‑1 (MQ=255)
                   gCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTtgtg                                                                                                                       <  4:343105/150‑1 (MQ=255)
                   gCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTtgtg                                                                                                                       >  2:335673/1‑150 (MQ=255)
                   gCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTtgtg                                                                                                                       <  1:335673/150‑1 (MQ=255)
                    cccGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGa                                                                                                                      >  4:796239/1‑150 (MQ=255)
                      cgcgCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGAtt                                                                                                                    <  1:150290/150‑1 (MQ=255)
                           cAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGt                                                                                                               >  3:612052/1‑150 (MQ=255)
                            aaTATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTc                                                                                                              <  2:111136/150‑1 (MQ=255)
                              tatCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCgg                                                                                                            <  1:47785/150‑1 (MQ=255)
                               atCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGt                                                                                                           <  1:1267277/150‑1 (MQ=255)
                                tCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTg                                                                                                          >  1:623118/1‑150 (MQ=255)
                                  aaCATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGc                                                                                                        >  3:197990/1‑150 (MQ=255)
                                  aaCATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGc                                                                                                        <  3:340915/150‑1 (MQ=255)
                                      ttGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTg                                                                                                    <  1:170096/150‑1 (MQ=255)
                                            ccATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATTAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTg                                                                                              <  3:100762/150‑1 (MQ=255)
                                              aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTgcg                                                                                            >  1:1286056/1‑150 (MQ=255)
                                                tGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTTCGCGTTACTCATCAGATCCTGTGCAAAACCAATCAGGTTATCAAAGTGTTTTTGATTTGCGTTGGTGGCGATCGAGGTGCgct                                                                                          >  4:33664/1‑150 (MQ=255)
                                                tGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCgct                                                                                          <  2:40450/150‑1 (MQ=255)
                                                tGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCgct                                                                                          <  2:1267077/150‑1 (MQ=255)
                                                tGCTCAGGCATCTTCTGAACGCTCATTCTCTGTCGTGGTAAATACGGAGGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAGCCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCgct                                                                                          <  4:758349/150‑1 (MQ=255)
                                                   tCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCgctgct                                                                                       <  4:82848/150‑1 (MQ=255)
                                                    cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGGCGGTGGCGTTGGCGGTGCGctgctg                                                                                      <  2:122915/150‑1 (MQ=255)
                                                     aGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTgg                                                                                     >  2:854601/1‑150 (MQ=255)
                                                       ggATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAg                                                                                   >  3:811323/1‑150 (MQ=255)
                                                       ggATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAg                                                                                   >  1:248200/1‑150 (MQ=255)
                                                             tctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTg                                                                             >  3:599910/1‑150 (MQ=255)
                                                             tctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTg                                                                             <  4:768645/150‑1 (MQ=255)
                                                              ctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTtg                                                                                                                         <  3:52761/105‑1 (MQ=255)
                                                              ctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTtg                                                                                                                         >  4:52761/1‑105 (MQ=255)
                                                              ctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGa                                                                            >  1:974163/1‑150 (MQ=255)
                                                                 aaCGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGctgctg                                                                                      >  2:1130802/1‑137 (MQ=255)
                                                                 aaCGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGctgctg                                                                                      <  1:1130802/137‑1 (MQ=255)
                                                                 aaCGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTgg                                                                                     <  1:1202351/138‑1 (MQ=255)
                                                                 aaCGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTgg                                                                                     >  2:1202351/1‑138 (MQ=255)
                                                                      tCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTtg                                                                                                                         >  4:224993/1‑97 (MQ=255)
                                                                      tCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTtg                                                                                                                         <  3:224993/97‑1 (MQ=255)
                                                                      tCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAg                                                                    >  4:760883/1‑150 (MQ=255)
                                                                      tCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGAAGCAACTGAAGCGTCAg                                                                    <  1:1312948/150‑1 (MQ=255)
                                                                      gcaaggtctTTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAg                                                                    >  4:44602/7‑150 (MQ=255)
                                                                         aTCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCaa                                                                 <  4:612052/150‑1 (MQ=255)
                                                                                tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAgctggc                                                          <  3:796239/150‑1 (MQ=255)
                                                                                   tGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTga                                                       >  2:197844/1‑150 (MQ=255)
                                                                                     gTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGATGGCGGTGCGCTGCTGTAGCAACTGAAGCGTCAGCAAAGCTGGCTgaag                                                     >  1:647113/1‑150 (MQ=255)
                                                                                        aaTAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCACCTGAGGCGTCAGCAAAGCTGGCTGAAGAAt                                                  <  4:576513/150‑1 (MQ=255)
                                                                                          tAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATaa                                                <  4:556292/150‑1 (MQ=255)
                                                                                          tAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATaa                                                >  1:1234943/1‑150 (MQ=255)
                                                                                                tgatgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACatat                                          <  2:754059/150‑1 (MQ=255)
                                                                                                  atgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGGAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCg                                        >  1:1038051/1‑150 (MQ=255)
                                                                                                   tgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGa                                       <  4:452839/150‑1 (MQ=255)
                                                                                                     cGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACt                                     >  2:454832/1‑150 (MQ=255)
                                                                                                      gACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACtt                                    >  1:84551/1‑150 (MQ=255)
                                                                                                       aCCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTgaag                                                     <  1:442379/132‑1 (MQ=255)
                                                                                                       aCCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTgaag                                                     >  2:442379/1‑132 (MQ=255)
                                                                                                          aCTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACgt                                >  1:191419/1‑150 (MQ=255)
                                                                                                                 tGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTg                           <  1:322958/148‑1 (MQ=255)
                                                                                                                 tGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTg                           >  2:322958/1‑148 (MQ=255)
                                                                                                                 tGCGCGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCg                         <  4:599910/150‑1 (MQ=255)
                                                                                                                  gcgcGTTACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCgg                        >  4:31529/1‑150 (MQ=255)
                                                                                                                        tACTCATCAGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTAGGTGTCTGCGGTGTTGc                  <  3:662546/150‑1 (MQ=255)
                                                                                                                           tcatcaGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCaa               >  2:560632/1‑150 (MQ=255)
                                                                                                                           tcatcaGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCaa               <  1:1130463/150‑1 (MQ=255)
                                                                                                                                aGATGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGa          <  3:760883/150‑1 (MQ=255)
                                                                                                                                   tGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCg                         <  4:263694/132‑1 (MQ=255)
                                                                                                                                   tGCTGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCg                         >  3:263694/1‑132 (MQ=255)
                                                                                                                                     cTGTTCAAAACCGATCAGGTTATCGGAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGct     <  2:1038051/150‑1 (MQ=255)
                                                                                                                                      tGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGctc    >  4:370/1‑150 (MQ=255)
                                                                                                                                      tGTTCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGctc    >  1:531148/1‑150 (MQ=255)
                                                                                                                                        ttCAAAACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTg  <  3:109841/150‑1 (MQ=255)
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GAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTG  >  NC_000913_3_hsa_tpiA/1578‑1863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑