Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA3,946,2890TC53.4% 125.9 / 157.6 116noncoding (2586/2904 nt)rrlC23S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base C (29/33);  total (55/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 116 total aligned reads displayed.

ACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGG  >  NC_000913_3_hsa_tpiA/3946142‑3946377
                                                                                                                                                   |                                                                                        
accccGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAg                                                                                        <  2:889120/147‑1 (MQ=2)
 ccccGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGa                                                                                       <  2:172610/148‑1 (MQ=2)
  cccGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGaa                                                                                      >  1:41321/2‑150 (MQ=2)
  cccGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGaa                                                                                      >  2:521487/2‑150 (MQ=2)
  cccGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGaa                                                                                      <  1:272628/149‑1 (MQ=2)
   ccGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAc                                                                                     >  2:954228/1‑150 (MQ=2)
    cGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcg                                                                                    >  3:283291/1‑150 (MQ=2)
    cGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcg                                                                                    >  2:862112/1‑150 (MQ=2)
    cGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcg                                                                                    >  2:596451/1‑150 (MQ=2)
     ggggATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcgt                                                                                   <  4:71621/150‑1 (MQ=2)
     ggggATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcgt                                                                                   <  3:711335/150‑1 (MQ=2)
     ggggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgt                                                                                   >  2:1229949/1‑150 (MQ=32)
      gggATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcgtc                                                                                  <  1:807432/150‑1 (MQ=2)
      gggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtc                                                                                  <  1:325141/150‑1 (MQ=32)
      gggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtc                                                                                  <  4:178876/150‑1 (MQ=32)
        gagaaCAGGCTGATACCGCCCAAGCGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcgt                                                                                <  4:43320/147‑1 (MQ=17)
        gATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGCGCTGGGTTCAGAAcgtcgg                                                                                >  2:693399/1‑149 (MQ=2)
        gATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcgtcgt                                                                                <  2:688367/150‑1 (MQ=2)
        gATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAAcgtcgt                                                                                <  3:40553/150‑1 (MQ=2)
        gATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcgt                                                                                <  2:162345/150‑1 (MQ=32)
         aTAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTg                                                                               >  4:526396/1‑150 (MQ=2)
          tAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTga                                                                              <  2:561526/150‑1 (MQ=2)
          tAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTga                                                                              <  2:496397/150‑1 (MQ=2)
          tAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTga                                                                              <  1:151923/150‑1 (MQ=2)
          tAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTga                                                                              <  3:720532/150‑1 (MQ=2)
          tAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTga                                                                              <  1:815352/150‑1 (MQ=32)
          tAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTga                                                                              <  4:729058/150‑1 (MQ=32)
          tAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTga                                                                              <  1:284345/150‑1 (MQ=32)
            aCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTgaga                                                                            <  1:292744/150‑1 (MQ=32)
             cAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGAc                                                                           >  2:290565/1‑150 (MQ=32)
              aGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACa                                                                          >  3:773828/1‑150 (MQ=32)
              aGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACa                                                                          >  1:233255/1‑150 (MQ=32)
              aGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACa                                                                          >  2:292681/1‑150 (MQ=32)
              aGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACa                                                                          >  2:201654/1‑150 (MQ=32)
              aGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACa                                                                          >  1:650700/1‑150 (MQ=32)
               ggCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAg                                                                         >  1:1089042/1‑150 (MQ=32)
               ggCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGACATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAg                                                                         >  1:689123/1‑150 (MQ=17)
                      ctcccccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGtttg                                                                     >  1:1236015/5‑145 (MQ=2)
                      ctcccccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGtttg                                                                     <  2:1236015/143‑3 (MQ=2)
                      aCCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTc                                                                  <  1:332606/150‑1 (MQ=32)
                       tcccccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTcc                                                                 >  2:365421/4‑150 (MQ=2)
                       tcccccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTcc                                                                 >  1:376457/4‑150 (MQ=2)
                         ccccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCt                                                               <  1:739599/149‑1 (MQ=2)
                          cccAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTa                                                              >  2:452245/1‑150 (MQ=32)
                          cccAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTa                                                              >  2:916583/1‑150 (MQ=32)
                          cccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTa                                                              >  1:227785/1‑150 (MQ=2)
                           ccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTAt                                                             >  1:644288/1‑150 (MQ=2)
                           ccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTAt                                                             >  1:209901/1‑150 (MQ=2)
                           ccAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTAt                                                             >  3:51346/1‑150 (MQ=2)
                            cAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATc                                                            >  3:58127/1‑150 (MQ=11)
                              agagTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTg                                                          <  1:292681/150‑1 (MQ=32)
                              agagTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGGCCCTATCTg                                                          >  4:431932/1‑150 (MQ=11)
                               gagTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTgt                                                         >  2:473381/1‑149 (MQ=2)
                                  ttCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTgttgt                                                      <  2:98585/150‑5 (MQ=2)
                                   tCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTg                                                     <  2:111481/150‑1 (MQ=32)
                                    tatatCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTgg                                                    <  2:791900/149‑1 (MQ=11)
                                    cATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTgg                                                    >  2:1145392/1‑150 (MQ=32)
                                     acatCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTggg                                                   <  3:571595/148‑1 (MQ=11)
                                             ggggaggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGgaa                                           <  4:58127/144‑2 (MQ=2)
                                             ggcggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGgag                                           >  1:587804/1‑150 (MQ=32)
                                               ggaggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGaaaa                                         >  1:559795/5‑150 (MQ=11)
                                               ggaggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGaaaa                                         <  1:124631/146‑1 (MQ=2)
                                               ggaggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGaaaa                                         <  1:311970/146‑1 (MQ=2)
                                               cggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGaa                                         <  1:41523/150‑1 (MQ=32)
                                                ggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAAc                                        >  3:373228/1‑150 (MQ=32)
                                                gaggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGaaaa                                         <  4:230515/146‑1 (MQ=2)
                                                gaggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGaaaa                                         >  3:230515/4‑149 (MQ=2)
                                                 gggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGgaaaatt                                       <  2:1027826/148‑6 (MQ=11)
                                                 aggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGgaaaatt                                       <  4:726950/148‑6 (MQ=2)
                                                 aggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGgaaaatt                                       <  3:96243/148‑6 (MQ=2)
                                                 aggtTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGaaaatt                                       <  3:7622/148‑3 (MQ=2)
                                                  tgtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGtt                                                                       <  2:1225510/117‑1 (MQ=33)
                                                  tgtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGtt                                                                       >  1:1225510/1‑117 (MQ=33)
                                                  tgtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTg                                      >  2:51204/1‑150 (MQ=32)
                                                  tgtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTg                                      >  2:582217/1‑150 (MQ=32)
                                                     ttGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTcc                                                                 <  1:182944/120‑1 (MQ=33)
                                                        gCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGaaaaga                                >  3:317007/1‑145 (MQ=11)
                                                        gCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGaaaaga                                >  3:360919/1‑145 (MQ=2)
                                                          aCCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGaaaagagc                              >  1:947289/1‑143 (MQ=2)
                                                            cTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCt                                                           <  2:883134/119‑1 (MQ=11)
                                                              cGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGgcgtt                                               <  2:217429/129‑3 (MQ=2)
                                                               ggtGTCGGCGCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGGGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTc                         <  4:589269/148‑1 (MQ=12)
                                                                aTGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTcc                        <  2:757217/150‑1 (MQ=32)
                                                                     ggCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTa                   >  4:739443/1‑150 (MQ=32)
                                                                        tcatcgCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGAAAAGAGCTGTTCCTAGTACga                >  1:1078398/1‑150 (MQ=2)
                                                                        tcatcaCATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACga                <  1:409321/150‑1 (MQ=32)
                                                                         catcgCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGAAAAGAGCTGTTCCTAGTACgag               <  1:479126/150‑1 (MQ=2)
                                                                           tcaCATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGagag             >  2:25160/1‑150 (MQ=32)
                                                                           tcaCATCCTGGAGCTGAAGTAGGGCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGagag             <  2:1226489/150‑1 (MQ=17)
                                                                              caTCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGga                                            >  2:794893/1‑116 (MQ=32)
                                                                              caTCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGga                                            <  1:794893/116‑1 (MQ=33)
                                                                                tCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGAAAAGAGCTGTTCCTAGTACGAGAGGACCg        <  3:401268/150‑1 (MQ=2)
                                                                                  cTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGGACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGa      >  4:113226/1‑150 (MQ=17)
                                                                                  cTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGa      >  4:407946/1‑150 (MQ=32)
                                                                                  cTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGa      >  4:407963/1‑150 (MQ=32)
                                                                                  cTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGa      <  4:430854/150‑1 (MQ=32)
                                                                                   tGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGAAAAGAGCTGTTCCTAGTACGAGAGGACCGGaa     >  1:1065499/1‑149 (MQ=2)
                                                                                    ggAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGt    <  1:916583/150‑1 (MQ=32)
                                                                                     gAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGACCTGTGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTg   <  3:359422/150‑1 (MQ=14)
                                                                                      aGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTTGGTCCCTATCTGTTGTGGGCGTTGGAAAATTGAAAAGAGCTGTTCCTAGTACGAGAGGACCGGAATgg  <  2:1065499/150‑1 (MQ=2)
                                                                                                                                                   |                                                                                        
ACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGG  >  NC_000913_3_hsa_tpiA/3946142‑3946377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑