Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,726,372	A→T	D117V (GAT→GTT)	nemR →	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,726,372	0	A	T	100.0%	315.0 / NA	89	D117V (GAT→GTT)	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (46/43);  total (46/43)

CAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCA  >  NC_000913_3_hsa_tpiA/1726227‑1726517                                                                                                                                                  |                                                                                                                                                  cAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTg                                                                                                                                               <  3:436547/150‑1 (MQ=255)  aGCGACGGACCGGGTTGACGCCATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGt                                                                                                                                              <  2:669655/150‑1 (MQ=255)   gCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTc                                                                                                                                             <  3:308984/150‑1 (MQ=255)           ctgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCagaag                                                                                                                                        >  4:117412/1‑147 (MQ=255)           ctgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCagaag                                                                                                                                        <  3:117412/147‑1 (MQ=255)            tgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGatat                                                                                                                                    <  4:469050/150‑1 (MQ=255)            tgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGatat                                                                                                                                    <  4:469017/150‑1 (MQ=255)            tgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGatat                                                                                                                                    >  2:801740/1‑150 (MQ=255)             gaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATg                                                                                                                                   >  4:647039/1‑150 (MQ=255)                    tgcAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAgcg                                                                                                                            >  2:319774/1‑150 (MQ=255)                      cAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGcgcg                                                                                                                          >  2:962062/1‑150 (MQ=255)                       aaTCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGa                                                                                                                         >  3:52513/1‑150 (MQ=255)                        aTCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCagaag                                                                                                                                        >  4:293673/1‑134 (MQ=255)                        aTCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCagaag                                                                                                                                        <  3:293673/134‑1 (MQ=255)                         tCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATg                                                                                                                       <  1:18712/150‑1 (MQ=255)                          ccGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATgg                                                                                                                      <  2:495482/150‑1 (MQ=255)                           cGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGa                                                                                                                     >  3:648600/1‑150 (MQ=255)                                aaGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAg                                                                                                                <  3:727685/150‑1 (MQ=255)                                   gTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTg                                                                                                             >  3:568963/1‑150 (MQ=255)                                        tcccGAGACCACATACGGGCTGATTACGAGCAAGCACTGAACCAGAGTTGCCGACATGGAACGATCCATTGATGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTcgc                                                                                                        >  2:1232813/3‑150 (MQ=255)                                         aCCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTcgcg                                                                                                       >  3:263235/1‑150 (MQ=255)                                             cgACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGt                                                                                                   <  1:767440/150‑1 (MQ=255)                                               aCCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGa                                                                                                 <  4:243686/150‑1 (MQ=255)                                                 cGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAATCTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATc                                                                                               <  2:323868/150‑1 (MQ=255)                                                 cGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGAAAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATc                                                                                               <  2:1181218/150‑1 (MQ=255)                                                    aTACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGcc                                                                                            <  3:647039/150‑1 (MQ=255)                                                      aCTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCt                                                                                          <  1:985362/150‑1 (MQ=255)                                                      aCTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCt                                                                                          <  2:764368/150‑1 (MQ=255)                                                            ttattaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctctc                                                                                    <  2:1085014/150‑1 (MQ=255)                                                            ttattaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctctc                                                                                    <  4:341013/150‑1 (MQ=255)                                                              attaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCtctctc                                                                                  >  2:1086086/1‑150 (MQ=255)                                                                taCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAg                                                                                <  1:962062/150‑1 (MQ=255)                                                                taCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCATTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctct                                                                                     <  4:386317/145‑1 (MQ=255)                                                                taCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCATTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctct                                                                                     >  3:386317/1‑145 (MQ=255)                                                                   cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGgcg                                                                             >  4:88709/1‑150 (MQ=255)                                                                   cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGgcg                                                                             >  1:817301/1‑150 (MQ=255)                                                                   cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGgcg                                                                             >  1:1133087/1‑150 (MQ=255)                                                                             tGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATg                                                                   <  4:309305/150‑1 (MQ=255)                                                                                aCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGcc                                                                >  3:758524/1‑150 (MQ=255)                                                                                 ccAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCaga                                                                                                                                          >  1:1057074/1‑75 (MQ=255)                                                                                 ccAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCaga                                                                                                                                          <  2:1057074/75‑1 (MQ=255)                                                                                 ccAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCATTCTGTCAGAAGATATGCGCAGCGCGa                                                                                                                         <  4:559812/92‑1 (MQ=255)                                                                                 ccAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCATTCTGTCAGAAGATATGCGCAGCGCGa                                                                                                                         >  3:559812/1‑92 (MQ=255)                                                                                   aGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCTCCCTGCGCTCTCAGGCGCTGGATGATGGCCGTg                                                             >  4:341850/1‑150 (MQ=255)                                                                                   aGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTg                                                             <  1:162357/150‑1 (MQ=255)                                                                                    gTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGt                                                                                                                                              <  4:758663/68‑1 (MQ=255)                                                                                    gTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGt                                                                                                                                              >  3:758663/1‑68 (MQ=255)                                                                                    gTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTga                                                            <  2:437252/150‑1 (MQ=255)                                                                                     ttttGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCGGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTgag                                                           >  1:93042/1‑150 (MQ=255)                                                                                           cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCAt                                                     >  2:496570/1‑150 (MQ=255)                                                                                            aaCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATaa                                                                                                                  <  3:550197/88‑1 (MQ=255)                                                                                            aaCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATaa                                                                                                                  >  4:550197/1‑88 (MQ=255)                                                                                            aaCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCAtt                                                    <  4:435067/150‑1 (MQ=255)                                                                                             aCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTg                                                   >  2:836578/1‑150 (MQ=255)                                                                                                tGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGttt                                                <  4:568963/150‑1 (MQ=255)                                                                                                  gAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAACGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTaa                                              <  2:699656/150‑1 (MQ=255)                                                                                                      cATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCtt                                          <  1:74791/150‑1 (MQ=255)                                                                                                          aGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTtgt                                      <  2:1178829/150‑1 (MQ=255)                                                                                                           gTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTtgtg                                     <  4:501733/150‑1 (MQ=255)                                                                                                            tGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGcgcg                                                                                                                          <  3:422988/64‑1 (MQ=255)                                                                                                            tGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGcgcg                                                                                                                          >  4:422988/1‑64 (MQ=255)                                                                                                            tGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTgg                                    >  4:131555/1‑150 (MQ=255)                                                                                                             ggTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGc                                   <  3:362229/150‑1 (MQ=255)                                                                                                              gTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCg                                  >  3:494736/1‑150 (MQ=255)                                                                                                              gTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCg                                  >  2:996246/1‑150 (MQ=255)                                                                                                               ttGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGa                                 >  4:404204/1‑150 (MQ=255)                                                                                                                   cTGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCg                             >  3:222597/1‑150 (MQ=255)                                                                                                                    tGACAGTAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGc                            >  4:486823/1‑150 (MQ=255)                                                                                                                          tAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAAc                      >  1:1013636/1‑150 (MQ=255)                                                                                                                          tAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAAc                      >  3:616089/1‑150 (MQ=255)                                                                                                                          tAAAACTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAAc                      >  3:576964/1‑150 (MQ=255)                                                                                                                           aaaaCTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATGAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACa                     >  2:308753/1‑150 (MQ=255)                                                                                                                            aaaCTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAg                    >  3:438763/1‑150 (MQ=255)                                                                                                                             aaCTCTCTGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAgg                   >  3:190391/1‑150 (MQ=255)                                                                                                                                  tctGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAGTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACaa              <  1:726134/150‑1 (MQ=255)                                                                                                                                   ctGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCGCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAg             <  3:341850/150‑1 (MQ=255)                                                                                                                                    tGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGt            >  3:402486/1‑150 (MQ=255)                                                                                                                                    tGCCGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGt            >  2:619310/1‑150 (MQ=255)                                                                                                                                      ccGATGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGAATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTTTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCACTGCAACAGGCACAAGTGc          <  2:1125346/150‑1 (MQ=255)                                                                                                                                      ccGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGc          >  3:709379/1‑150 (MQ=255)                                                                                                                                       cGAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCt         >  2:165914/1‑150 (MQ=255)                                                                                                                                        gAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCtt        >  1:405636/1‑150 (MQ=255)                                                                                                                                        gAAGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCtt        <  2:1186253/150‑1 (MQ=255)                                                                                                                                          aGTGTGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTa      <  1:1086086/150‑1 (MQ=255)                                                                                                                                           gtgtGCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTAc     <  3:88709/150‑1 (MQ=255)                                                                                                                                              tgCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCt                                                                           <  4:552616/77‑1 (MQ=255)                                                                                                                                              tgCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCt                                                                           >  3:552616/1‑77 (MQ=255)                                                                                                                                              tgCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCa  >  3:23194/1‑150 (MQ=255)                                                                                                                                              tgCGTTCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCa  >  4:153337/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCA  >  NC_000913_3_hsa_tpiA/1726227‑1726517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑