Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913_3_hsa_tpiA	1,158,725	Δ96 bp	32.8%	coding (857‑952/1434 nt)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1158724	19 (0.590)	11 (0.340)	10/276	NT	32.8%	coding (856/1434 nt)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
	?	NC_000913_3_hsa_tpiA	1158821 =	26 (0.810)					coding (953/1434 nt)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component

TTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/1158585‑1158724 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGC  >  NC_000913_3_hsa_tpiA/1158821‑1158924                                                                                                                                                                                                                                                      TTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCCCGAAAATGGGCGGTATTATGATCTTTGCAGGCC                                                                                                 >  2:275854/1‑149                     CTGGGGGTAAACTGTCTGGTTGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGATAGTGGGCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATT                                                                             <  2:117834/149‑1                      TGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGGATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGGATTC                                                                            >  1:571209/1‑149                               ACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGA                                                                   >  2:340525/1‑149                                       GTGGCTTCCTGTTCAAAATGGACGGTCTGCCAGCTGCCGCAATTGCTATCTGTCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGTCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGAC                                                           <  2:543046/149‑1                                       GTGGCTTCATGTGCAAACGGTACGGTCTGCCGGCTGCGGCAATTGGTATCTGGCACGCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGAC                                                           <  2:215966/149‑1                                                      AAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCG                                            <  1:227762/149‑1                                                        attTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCA                                          <  1:534073/146‑1                                                            ACGGCCAGCCAGTGGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGT                                      <  2:278789/149‑1                                                                                            cacctTGCTAAACCAGAAAACCGCGCGAAAGTTGGCCGTATTATGATCTTGGCACGCCTGGCATTCCCCTTCCGTATCCTCCGGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAG      <  1:84758/144‑1                                                                                                CTGCTAAACCAGAAAACCGCCCGAAAGTGGGCGGTATTATGATCTTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGC  <  1:191358/149‑1                                                                                                                                                                                                                                                      TTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/1158585‑1158724 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGC  >  NC_000913_3_hsa_tpiA/1158821‑1158924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.