Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	264,098	G→T	100%	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	264,098	0	G	T	100.0%	93.0 / NA	34	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/18);  total (16/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGA  >  NC_000913_3_hsa_tpiA/263952‑264237                                                                                                                                                   |                                                                                                                                            cGGGCTCGCAGGCGCAGTGTACTCGCCGGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAg                                                                                                                                           <  1:137894/149‑1 (MQ=255)    gCTCCCAGGCGCAGTGCAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                                                                                                        <  1:309553/149‑1 (MQ=255)        ccgcgcgcAGTGGAATCGCTCGGTGCGGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCATTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                    <  2:289820/145‑1 (MQ=37)        ccAGGCGCAGTGGAATCCCCCGCCGCAGGCCCCTGGTAGGGTTGCTTATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                                    <  1:322333/149‑1 (MQ=39)          aGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                                  <  1:204097/149‑1 (MQ=255)                   gtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCACCACTATTCTCAGTTCAGGGGGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGCTCTCCgg                                                                                                                         >  1:241174/1‑149 (MQ=255)                    tAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTACACTATCCTCAGTTAAGAGTGATGTGCATCACGCAGGAATTGGGTCTATTGTGGACCTGGCGGCCTTCTCCGCATAGGGTTAGAACTACGGCAATATACGGa                                                                                                                        >  2:207109/1‑149 (MQ=255)                                gCATGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTAAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCg                                                                                                            <  2:497124/149‑1 (MQ=255)                                 cAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGGATAGCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCgg                                                                                                           >  1:478998/1‑149 (MQ=255)                                                gTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGc                                                                                            >  1:27442/1‑149 (MQ=255)                                                       aTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTGTAGGGCCCATTGCGGTCGTGGCGGCGTTCGGCGTATACGGCTAGGACTCCGGCGATATTCGGAACGTCCCTGCCGGTGTACTGCCAGACGCTACCGTg                                                                                     >  2:182965/1‑149 (MQ=25)                                                       aTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTg                                                                                     >  1:437346/1‑149 (MQ=255)                                                          aCAGTTCTCCACTCTTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCa                                                                                  <  1:445913/149‑1 (MQ=255)                                                                    aCTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGGACGTCCCTGCCGGTGTAATGCCAGACGCTACCGTGCCACTGGTATTTg                                                                        >  2:501735/1‑149 (MQ=255)                                                                     gtATTGTGAGTTGGGGGGGAGGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGc                                                                       <  2:440129/148‑1 (MQ=21)                                                                        ttGTCAGTTCAGGGTGATGCTCATCAGGCAGGAATCGTGCGCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCgg                                                                    >  1:610626/1‑149 (MQ=21)                                                                        ttGTCAGTTCAGGGTGATGCTCATCAGGCAAGTTCAGCGCCCGTTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCgg                                                                    >  1:610633/1‑149 (MQ=21)                                                                               ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCTATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                             <  1:239681/149‑1 (MQ=255)                                                                               ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                             >  1:38540/1‑149 (MQ=255)                                                                                      tGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAGTTGCCGGTGCAGTGGCGAAAg                                                      >  1:264692/1‑149 (MQ=255)                                                                                          gCTCATCAGGCATGTATAGGGTCCATTTGCGTCCTGTCGTCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTc                                                  <  1:77260/149‑1 (MQ=18)                                                                                            tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                <  1:524527/149‑1 (MQ=255)                                                                                                   ggaggGATAGGGCCCATTGGGGTCCTGGCGGCGTTCGGCTGATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGGGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                         <  2:503023/147‑1 (MQ=18)                                                                                                                ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGCTATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGCAAGATTCAGGGCTCAGGATGCTGGCGGta                            >  2:253784/1‑149 (MQ=17)                                                                                                                   ttGCGGTCCGGGTGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCGGCCGGTGTAATGACAGCCGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTatat                         <  2:24897/149‑1 (MQ=17)                                                                                                                    tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc                        <  1:606658/149‑1 (MQ=255)                                                                                                                         tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCAt                   >  1:528435/1‑149 (MQ=255)                                                                                                                           cTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCGACCGTGCCACTGGGATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGCCACTg                 >  1:365640/1‑149 (MQ=17)                                                                                                                           cTGGCGGCGTTCGGCGTATACCGCTAGGACTACGGCGATATCCGGACCGTCCCTGCCGGTGTCATGACAGACGCTACCGTGCCCCTGGTATTTGCCGGTGCCGTAGCGGAAGATGCGGGGTTCAGTATGGTGGCGGTATATCTTCATTg                 >  2:207106/1‑149 (MQ=17)                                                                                                                               gggcgtgtggcgtgtACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCt             <  2:462090/148‑1 (MQ=255)                                                                                                                                    ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCGGCGtt        >  1:555475/1‑149 (MQ=255)                                                                                                                                      cGGCGTATACCGCTGGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTa      <  1:444330/149‑1 (MQ=255)                                                                                                                                        ggggatgccGCTAGGACTGCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTTCCAGCGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACt    <  1:455425/142‑1 (MQ=14)                                                                                                                                          gTATACCGCTAGGGCTCCGGTGATCTCCGGAACGTCCCGGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATGTGACGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGa  <  1:503320/149‑1 (MQ=14)                                                                                                                                                   |                                                                                                                                            CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGA  >  NC_000913_3_hsa_tpiA/263952‑264237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑