Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 2,817,170 C→A 100% E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,817,1700CA100.0% 104.7 / NA 37E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/19);  total (18/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_hsa_tpiA/2817029‑2817315
                                                                                                                                             |                                                                                                                                                 
gTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGATTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTTCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcccc                                                                                                                                            >  2:298273/1‑149 (MQ=255)
 ttGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGCACTGATGGTGATTGAAGTGATCGGCCGCGACGACGGCGGCCAAATACTCGAGTAATCCCAGCTGCGCCGGCAATGACTCAACGATGGCACTATAAGTCCCCg                                                                                                                                           >  2:565502/1‑149 (MQ=255)
         ccATACGCTGCGCGCACAACAAAAATGTGTCTGGAGCGGGTTTATGGTGTTTGACGTGATCCGCAGCGCCGCCGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACa                                                                                                                                   >  2:252841/1‑149 (MQ=255)
         ccATACGCTGCGCGAACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGATTGACGTGAACGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTTGGCAAGCAATGCCTCAGCGCTGGCGCTTTAACTCCCCGATCCTAct                                                                                                                                   >  2:10182/1‑148 (MQ=255)
           agaCGCCGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGc                                                                                                                                 <  1:78221/147‑1 (MQ=255)
                            caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                >  1:78092/1‑149 (MQ=255)
                            caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                <  1:370314/149‑1 (MQ=255)
                            caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAACGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                <  2:531348/149‑1 (MQ=255)
                                 aTGTGGCGGGCGCCGGTTTATGGTGTTTGACGTCATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCaa                                                                                                           <  1:151234/149‑1 (MQ=255)
                                      tCTGGCGGGGGTTTATGGGGTTTGGCGCGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtttt                                                                                                      <  2:504779/149‑1 (MQ=255)
                                            gcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcaccac                                                                                                <  1:527521/149‑1 (MQ=255)
                                                     tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTGAAACGCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAag                                                                                       >  1:295846/1‑149 (MQ=255)
                                                      ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                      <  1:460828/149‑1 (MQ=255)
                                                           ctGACGTGATCGGCAGCGACGACGGGCTCAAAATAATGGCGTACTCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTCAACTCCCCGTTCCTACAGCCATTGGGCGACGCCCAAGCAAACTTTTCACCCCATTAACAAGAGGAAg                                                                                 <  2:610583/148‑1 (MQ=255)
                                                              aCGTGATCGTCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg                                                                              <  2:113903/149‑1 (MQ=255)
                                                                 tGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCACTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACCTCAACAAGAGGAAGCGGTTc                                                                           >  1:392560/1‑149 (MQ=255)
                                                                    tCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                        <  1:240394/149‑1 (MQ=255)
                                                                       gCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATACCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATCCCACGTTTACCCCACATCACCAAGAGGAACCGGATCGACGCt                                                                     >  1:92731/1‑149 (MQ=255)
                                                                              cgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagc                                                              <  2:79969/149‑1 (MQ=255)
                                                                                ccgGCGTCAAAATAATGGCGTAACACCAGGTGCCCCAGCAATGCCTCAGCGATGGCGCTTTACCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGTAAGCGGTTCGACGCTATCcagcag                                                            <  2:431769/148‑1 (MQ=255)
                                                                                        aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACGTCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATCcttc                                                    >  2:196977/1‑149 (MQ=255)
                                                                                            taatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAAAGGAGGCGGTTCGGCGCTATCCAGCAGCATACTTCTTAc                                                >  1:415282/1‑149 (MQ=255)
                                                                                              atGGAGTAATCCCAGGTGCGCCAGCAATACCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGGCGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACGTCTTACTg                                              >  2:468657/1‑149 (MQ=255)
                                                                                               tGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGc                                             >  2:586708/1‑149 (MQ=255)
                                                                                                  cGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTc                                          <  1:70046/149‑1 (MQ=255)
                                                                                                  cGTAATCCCAGGTGCGCCAGCAATGACTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTc                                          >  1:237576/1‑149 (MQ=255)
                                                                                                     actCCCAGGTGCGCTAGCAATGCCTCAGCCGTGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGt                                       <  2:76599/147‑1 (MQ=255)
                                                                                                      aTCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGAAAGCTGTTAGACGGTATCCAGCAGCATACTTCTTACTGCTTCTGtt                                      >  1:321656/1‑149 (MQ=255)
                                                                                                        cccAGGTGCGCCAGCAATGCCCCGGCGATTGCGCTTTAACTCCCCGTTCCTACAGCCATTGGACGAAGACCATGCCAACTTTTCACCACAAAAACCAGAGGCAGCGGATCCATGCTATCCCACAGCCTACTACTCACTACTACTGtttt                                    >  2:230/1‑149 (MQ=255)
                                                                                                        cccAGGGGCGCCAGCAATGCCTCCGCGATGGCGCTTTACCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttt                                    <  2:534570/149‑1 (MQ=255)
                                                                                                              tGCGCCAGCAATGGCTCAGCCATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGTAGCATAGTGCTTACTGCTTCTGTTTTTTCAcg                              <  1:301110/149‑1 (MQ=255)
                                                                                                                   cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTa                         >  1:395041/1‑149 (MQ=255)
                                                                                                                    aGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGACGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTaa                        >  1:505322/1‑149 (MQ=255)
                                                                                                                         tGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCAt                   >  1:599880/1‑149 (MQ=255)
                                                                                                                                gCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAACCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTc            <  1:467412/149‑1 (MQ=255)
                                                                                                                                       cgcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCg     <  2:426878/149‑1 (MQ=255)
                                                                                                                                          tttAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc  <  1:502825/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_hsa_tpiA/2817029‑2817315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑