Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	389,925	(T)6→7	intergenic (‑198/‑326)	hemB ← / → yaiT	5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	389,919	1	.	T	100.0%	110.5 / NA	32	intergenic (‑192/‑332)	hemB/yaiT	5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (17/15);  total (17/15)

CAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑TTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGA  >  NC_000913_3_hsa_tpiA/389774‑390057                                                                                                                                                   |                                                                                                                                           cAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑ttt                                                                                                                                         <  1:247745/149‑1 (MQ=255)      tAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttg                                                                                                                                    <  2:335812/149‑3 (MQ=255)           aTGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGATATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGtt                                                                                                                                <  2:382713/149‑1 (MQ=255)            tGTTTTACGAAAGTTGTCGGGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGttt                                                                                                                               <  2:6880/149‑1 (MQ=255)             gTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTg                                                                                                                              >  1:352856/1‑149 (MQ=255)               tttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGt                                                                                                                            >  1:154548/1‑149 (MQ=255)                ttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATACACACTGAATTTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGtt                                                                                                                           <  1:188073/149‑1 (MQ=255)                  actaaaGTTGGCGCGAGGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTCATAGCTCTTTGTTATTATTTTTTTGGAGTTTGGTTgc                                                                                                                         <  1:208549/146‑1 (MQ=255)                   cGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTgcg                                                                                                                        <  1:226063/149‑1 (MQ=255)                            tCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaa                                                                                                               >  2:353187/1‑149 (MQ=255)                                    ttGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaata                                                                                                       >  1:131512/1‑149 (MQ=255)                                    ggtaaaagaagAGGATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaata                                                                                                       <  2:9369/144‑1 (MQ=255)                                       aCAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGACTAAtt                                                                                                    >  1:263885/1‑149 (MQ=255)                                       aCAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAAtt                                                                                                    >  1:360950/1‑149 (MQ=255)                                              gagaATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTTAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGATATAAATTGAATAATTAAAACtt                                                                                             >  1:354693/1‑149 (MQ=255)                                                gaATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttg                                                                                           >  1:303400/1‑149 (MQ=255)                                                 aaTGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTGATTTTGTTTGCGCTGCTATAACTTGAATTATTAACACtttcg                                                                                          >  2:86068/1‑147 (MQ=255)                                                                aGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGa                                                                           <  2:154548/149‑1 (MQ=255)                                                                        tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgttg                                                                                        >  1:389197/1‑128 (MQ=255)                                                                        tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgttg                                                                                        <  2:389197/128‑1 (MQ=255)                                                                        tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGATGCATATTCTTAGGATATTTATc                                                                   >  1:354309/1‑149 (MQ=255)                                                                        tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTAGTTATTATTTTTTTGGAGTTTGGTTGCGCTGTTATAAATTGAATACATAAAAATTTTTGTCATATCAATTGGATACTTATc                                                                   >  2:164823/1‑149 (MQ=255)                                                                         ttttATTAGAATCAGCAATAATTTTATATTCTCTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCt                                                                  <  2:187501/149‑1 (MQ=255)                                                                                aGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgt                                                           <  1:382576/149‑1 (MQ=255)                                                                                 gAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtg                                                          >  1:382626/1‑149 (MQ=255)                                                                                                      tCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGt                                     <  2:303400/149‑1 (MQ=255)                                                                                                            aaaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCtt                               >  2:274304/1‑149 (MQ=255)                                                                                                               atatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaa                            >  2:31654/1‑149 (MQ=255)                                                                                                                    tttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTtattat                                         <  2:85391/131‑1 (MQ=255)                                                                                                                    tttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTtattat                                         >  1:85391/1‑131 (MQ=255)                                                                                                                    tttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACa                       <  2:360950/149‑1 (MQ=255)                                                                                                                                      cTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGt     >  1:96326/1‑149 (MQ=255)                                                                                                                                      cTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGt     <  1:94868/149‑1 (MQ=255)                                                                                                                                         ttGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGa  <  2:184865/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                           CAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑TTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGA  >  NC_000913_3_hsa_tpiA/389774‑390057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑