New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 137328419 (0.800)4 (0.180) 4/270 2.6 17.4% coding (63/882 nt) ycjO putative sugar ABC transporter permease
?NC_000913_3_hsa_tpiA = 1373292 20 (0.890)coding (71/882 nt) ycjO putative sugar ABC transporter permease
Rejected: Frequency below/above cutoff threshold.

ACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCT  >  NC_000913_3_hsa_tpiA/1373148‑1373439
                                                                                                                                                |                                                                                                                                                   
actacGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTTACATTTTCCTTTGTCTCTGCTGATTCTCGCGCCCAGCTAATTCCTgctg                                                                                                                                                 <  2:150483‑M2/149‑5 (MQ=255)
                  cAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGgtggcgtgg                                                                                                                               <  1:256381/149‑1 (MQ=255)
                          aCTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGACGTTCAGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGccattgtt                                                                                                                       >  2:329367/1‑143 (MQ=255)
                            tGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCTTGTCGTTCCGATATGCCCTTGGCGCCGCTCCTACCCGCCCCCAGCTTATTACTGCCGGGCGGTCTGATGGCGCGGCCGATGgtgc                                                                                                                     >  2:56291/1‑148 (MQ=255)
                                        tCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATcccca                                                                                                         >  2:316134/1‑145 (MQ=255)
                                                cACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGGAATCAGtttt                                                                                                 >  1:38100/1‑149 (MQ=255)
                                                         caggacaggaATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTg                                                                                        <  2:160786/149‑1 (MQ=255)
                                                             acaggaATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGc                                                                                    >  1:280828/1‑149 (MQ=255)
                                                                 gaATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCCGCTTATTACTGCTGGGCGGTCTGGTGGCGGGGCCGATGGTGTCGAATAGCGAAATCAGTTTTTTCAGTCCGCCGCTCaa                                                                                >  2:409608/1‑149 (MQ=255)
                                                                              aCAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCATCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAg                                                                   <  1:308585/149‑1 (MQ=255)
                                                                                aGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGATTTCAGTGTTTTACGTCTGCCGCTGCATCCCAACATCGAGTc                                                                 >  1:244242/1‑149 (MQ=255)
                                                                                  gCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCCGTTTTTTACGTCTGCCGCTCCATCTCAAAATCGCGTccg                                                               >  2:223015/1‑147 (MQ=255)
                                                                                  gCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCaa                                                               >  2:298162/1‑149 (MQ=255)
                                                                                          cAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTttgtt                                                       <  1:298162/149‑1 (MQ=255)
                                                                                                           cccTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCGCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTAtgt                                      <  2:280828/149‑1 (MQ=255)
                                                                                                                  cgcTGCTGCTTCTCGCGCCCAGCTTATTGCTCCGGAGCCGTCTGGTGGAGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATc                               <  1:56291/149‑1 (MQ=255)
                                                                                                                     tgctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCctc                            >  2:288305/1‑149 (MQ=255)
                                                                                                                              tcGCGCCCAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagcctgttcattg                                                                                    <  2:285228‑M2/84‑66 (MQ=255)
                                                                                                                              tcGCGCCCAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagcctgttcattg                                                                                    >  1:285228‑M2/1‑19 (MQ=255)
                                                                                                                                  gcCCAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagcctgttcattgcggattcctgtcctgggtttagtgctgttcgatcagttcatccagttttttctggctcgcgtgcagcgt               <  1:369274‑M2/149‑135 (MQ=255)
                                                                                                                                   cccAGCTTATTACTgctgggcgcgagaagcagcagcgcaaagggcatatcggaacgacctgaaaaaagccggttcattgcggattcctgtcctgggtttagtgctgttcgatcagttcatccagttttttatggctcgcgtgcggattc              <  2:225678‑M2/149‑136 (MQ=255)
                                                                                                                                     cAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCt            >  1:181856/1‑149 (MQ=255)
                                                                                                                                     cAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCt            >  2:295368/1‑149 (MQ=255)
                                                                                                                                      aGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGCTCCCGGCTTCTg           >  2:73331/1‑149 (MQ=255)
                                                                                                                                               ctgctgGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCt  <  2:282096/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCT  >  NC_000913_3_hsa_tpiA/1373148‑1373439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.