Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,756,430	G→T	G245C (GGC→TGC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,756,430	0	G	T	100.0%	52.4 / NA	17	G245C (GGC→TGC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/11);  total (6/11)

TGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGC  >  NC_000913_3_hsa_tpiA/1756291‑1756564                                                                                                                                            |                                                                                                                                       cgagcttATCGGAATCCCTGCGCTACCGACAGGGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGATAACTAGGCAGGCCATAGCTACGTCGACTCCATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTgg                                                                                                                               <  2:89561/144‑1 (MQ=255)      ttATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTa                                                                                                                          <  2:307005/149‑1 (MQ=255)           gAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGTCATCATCGTTGCGCGTTGCGATCTGCGTGTAAAAAt                                                                                                                     >  2:157657/1‑149 (MQ=255)              aTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATccc                                                                                                                  <  2:88052/149‑1 (MQ=255)                         gcTGGAAGCGCACGGCGGCGAAGACAGCCGCGTCAGCTCCAAAATCGAAAGCCAGGAAGGCCTCAACAGCTTCGACGAAATGCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGaagaag                                                                                                       <  2:181531/148‑1 (MQ=255)                            gAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAACCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCGGGAAGAAGAAGTTa                                                                                                    >  1:132437/1‑149 (MQ=255)                               aGCGTAGGGCGGTGAAAACATCCCCATTACATCCAAAAAGGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAGTCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCt                                                                                                 <  2:2993/149‑1 (MQ=255)                               aGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCt                                                                                                 <  1:152356/149‑1 (MQ=255)                                            gAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTc                                                                                               >  1:24614/1‑138 (MQ=255)                                            gAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTc                                                                                               <  2:24614/138‑1 (MQ=255)                                              aaaCCTACACATCAGCTGCAAAAGCGAAAACCAGGAAGGCGTCAACAACTTTGACGAAATCCTCGAAGCGTCTGACGGCAGCATGGTTGCGCGTTGCGACCTGGGGGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga                                                                                  <  2:167151/149‑1 (MQ=255)                                                      acaTCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCTCCGAGAAGATGATGATCGaa                                                                          >  1:64224/1‑149 (MQ=255)                                                              tcCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGAGGATGATCGAAAAATGTAt                                                                  >  1:148322/1‑149 (MQ=255)                                                                                        tgacatcTTCGACGAAATCCTCGAAGCCTCGGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                        <  2:210690/147‑1 (MQ=255)                                                                                                                aGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGa                <  2:244531/149‑1 (MQ=255)                                                                                                                aGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGa                <  2:148322/149‑1 (MQ=255)                                                                                                                              tcatGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCAAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTGAGCACTGCGACCCAGATGCTGGATTCCATGGTCAAAAACgcgcgc  >  1:352069/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                       TGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGC  >  NC_000913_3_hsa_tpiA/1756291‑1756564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑