Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,679,5250GT96.7% 205.7 / ‑6.1 62A1074D (GCT→GAT) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (31/28);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT  >  NC_000913_3_hsa_tpiA/2679379‑2679669
                                                                                                                                                   |                                                                                                                                                 
tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCg                                                                                                                                                >  2:9073/1‑150 (MQ=255)
      ttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTaa                                                                                                                                          >  3:73667/1‑150 (MQ=255)
       tttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGCATTact                                                                                                                                         >  2:509732/1‑148 (MQ=255)
        ttattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATc                                                                                                                                        >  1:251264/1‑150 (MQ=255)
          atacacaacGGCTAATTCTAATTATTTTGTGTTGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGc                                                                                                                                      <  1:78428/146‑1 (MQ=255)
                            tAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGc                                                                                                                    >  1:431269/1‑150 (MQ=255)
                             aaTTGTTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGcc                                                                                                                   >  2:813374/1‑149 (MQ=255)
                             aaTTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGATAAAAATAAAAGGCATCACGGTGGTGAATAATGCGCCTTTCAGCCAAACCGGATGAATCTCCCTCAATTCACCGCGACCa                                                                                                                   >  2:788835/1‑150 (MQ=255)
                               ttattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                                                                                                                 >  2:801628/1‑150 (MQ=255)
                               ttattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                                                                                                                 >  2:811497/1‑150 (MQ=255)
                                        gatgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTg                                                                                                       >  1:635946/1‑150 (MQ=255)
                                        gatgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTg                                                                                                       >  1:89526/1‑150 (MQ=255)
                                         ttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTgc                                                                                                      >  1:550096/2‑150 (MQ=255)
                                         atgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGGATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTgc                                                                                                      <  2:636955/150‑1 (MQ=255)
                                          tgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTgcg                                                                                                     >  3:478961/1‑150 (MQ=255)
                                           gaGCGGCAGCGCG‑TGCCGCTAAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTgcgc                                                                                                    <  2:721875/150‑1 (MQ=255)
                                              cGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAAc                                                                                                 >  1:595522/1‑150 (MQ=255)
                                              cGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAAc                                                                                                 >  1:97940/1‑150 (MQ=255)
                                                gCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAACGGCCTAACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCt                                                                                               >  1:233741/1‑150 (MQ=255)
                                                  aGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTcc                                                                                             >  3:528159/1‑150 (MQ=255)
                                                       g‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAg                                                                                        <  2:333328/150‑1 (MQ=255)
                                                          gCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGTCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTc                                                                                      <  1:656963/150‑1 (MQ=255)
                                                              cTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCcgc                                                                                  <  3:221540/150‑1 (MQ=255)
                                                              cTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCcgc                                                                                  <  1:63812/150‑1 (MQ=255)
                                                               tCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCcgcg                                                                                 <  2:595522/150‑1 (MQ=255)
                                                                 aaaTATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGa                                                                               >  2:842838/1‑150 (MQ=255)
                                                                   atatTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACg                                                                             >  2:833624/1‑150 (MQ=255)
                                                                           gTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATc                                                                     >  3:25853/1‑150 (MQ=255)
                                                                           gTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATc                                                                     <  1:463288/150‑1 (MQ=255)
                                                                            ttAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCg                                                                    >  3:169243/1‑150 (MQ=255)
                                                                              aaTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGcc                                                                  <  1:9073/150‑1 (MQ=255)
                                                                              aaTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGAGACCATA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGcc                                                                  <  1:843475/150‑1 (MQ=255)
                                                                              aaTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGACGTCAACTGCGCGAGCGCAGAGCACA‑ATTTCAAAACCTCCCCCGGTTCAGCGGACGCCAGCATCGcc                                                                  >  3:378471/1‑150 (MQ=255)
                                                                                tAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGccac                                                                <  2:251264/150‑1 (MQ=255)
                                                                                  gggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGccacca                                                              <  1:811497/150‑1 (MQ=255)
                                                                                   ggTTTAGTGAACGCGGTTATAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGATAAGCCGGTTTAATCGCCGTCAAGTCAGCGCGAGCAGA‑CTCATATTCCACAACCTCCCCTAGACCCGCTTACACCAGCATCGGCACCAt                                                             >  4:116220/1‑150 (MQ=255)
                                                                                   ggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCAt                                                             <  4:82209/150‑1 (MQ=255)
                                                                                     tttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCa                                                           >  4:114957/1‑150 (MQ=255)
                                                                                      ttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCCATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAg                                                          >  4:342998/1‑150 (MQ=255)
                                                                                          tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGTGTGCCGCGGACGCCAGCATCGCCACCATCAGGCAt                                                      <  2:431269/150‑1 (MQ=255)
                                                                                            aaCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAttt                                                    <  1:344697/150‑1 (MQ=255)
                                                                                            aaCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAttt                                                    >  1:582925/1‑150 (MQ=255)
                                                                                            aaCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAttt                                                    >  4:1523/1‑150 (MQ=255)
                                                                                               gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGcca                                                                 <  2:428603/134‑1 (MQ=255)
                                                                                               gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CGCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGcca                                                                 >  1:428603/1‑134 (MQ=255)
                                                                                                cgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCt                                                <  3:1523/150‑1 (MQ=255)
                                                                                                  gNTAAAAATAAAAGGCATCACGGTGGNGAATAATGCCGCTTTNGGCNAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCttt                                              >  1:574236/1‑150 (MQ=255)
                                                                                                    tAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAt                                            <  4:73667/150‑1 (MQ=255)
                                                                                                                    cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATc                            >  4:473023/1‑150 (MQ=255)
                                                                                                                       ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGcc                         <  4:528657/150‑1 (MQ=255)
                                                                                                                          ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATa                      <  2:84588/150‑1 (MQ=255)
                                                                                                                             gAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATGCATCGCTATCATA‑CTCATAGTGCTCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAg                   <  1:509732/150‑1 (MQ=255)
                                                                                                                              aataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGc                  <  1:801628/150‑1 (MQ=255)
                                                                                                                                taatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGa                >  3:431689/1‑150 (MQ=255)
                                                                                                                                    gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGacaac            <  1:833624/150‑1 (MQ=255)
                                                                                                                                      cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa          <  2:537722/150‑1 (MQ=255)
                                                                                                                                       gCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAg         <  1:366817/150‑1 (MQ=255)
                                                                                                                                           tCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCCGATGTTGTAAATTGTCATACAGCGACAACAAGTCAg     <  4:134372/150‑1 (MQ=255)
                                                                                                                                            cGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAgg    <  2:233741/150‑1 (MQ=255)
                                                                                                                                             ggCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGt   <  1:282736/150‑1 (MQ=255)
                                                                                                                                              gCCAATCCTGATCAATCGCAGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCAGCGCCACCATCAGCCCTTTTTCTTTATGCTCCTGTCGTAAATCTCCATACAGCGACAACAAGTCAGGtt  >  4:123305/1‑150 (MQ=255)
                                                                                                                                              gCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGCCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTACATCGCCATCCAGCGACAACAAGTCAGGtt  >  2:484590/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGA‑CTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT  >  NC_000913_3_hsa_tpiA/2679379‑2679669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑