Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,183,195 A→T T657S (ACC→TCC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,183,1950AT98.7% 272.5 / ‑3.6 78T657S (ACC→TCC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (40/37);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

TATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAA  >  NC_000913_3_hsa_tpiA/4183050‑4183341
                                                                                                                                                 |                                                                                                                                                  
tATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCca                                                                                                                                                <  3:433653/150‑1 (MQ=255)
 aTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCcag                                                                                                                                               >  1:96439/1‑150 (MQ=255)
  tCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCcagc                                                                                                                                              <  2:71436/150‑1 (MQ=255)
  tCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCcagc                                                                                                                                              <  2:398652/150‑1 (MQ=255)
   cGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGNCGCNACCAGGTTNACTACATGGACGTATCCTCCcagca                                                                                                                                             <  3:496338/150‑1 (MQ=255)
   cGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACGTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCcagca                                                                                                                                             <  1:514365/150‑1 (MQ=255)
    gaagaaGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCcagcag                                                                                                                                            >  1:326108/1‑150 (MQ=255)
      agaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAggt                                                                                                                                          >  4:245627/1‑150 (MQ=255)
      agaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAggt                                                                                                                                          <  3:274470/150‑1 (MQ=255)
       gaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAggtg                                                                                                                                         <  1:749964/150‑1 (MQ=255)
        aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAggtgg                                                                                                                                        >  2:821077/1‑150 (MQ=255)
           gCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAACAGGTGGTAt                                                                                                                                     >  3:169493/1‑150 (MQ=255)
             aaCTACGTTATCGCCTAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGTCTACATGGACGTATCCTCCCAGCAGGTGGTATcc                                                                                                                                   >  4:134979/1‑150 (MQ=255)
             aaCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATcc                                                                                                                                   >  4:442743/1‑150 (MQ=255)
              aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCg                                                                                                                                  >  1:660926/1‑150 (MQ=255)
               cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTAtatat                                                                                                                                 >  1:12296/1‑146 (MQ=255)
                    ttATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTg                                                                                                                            >  1:378147/1‑150 (MQ=255)
                          cccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                                      <  2:346385/150‑1 (MQ=255)
                          cccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                                      >  3:512636/1‑150 (MQ=255)
                               gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATc                                                                                                                 >  4:469667/1‑150 (MQ=255)
                               gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATc                                                                                                                 <  3:442743/150‑1 (MQ=255)
                                cGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATcc                                                                                                                <  1:773614/150‑1 (MQ=255)
                                        aaCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTg                                                                                                        >  2:155128/1‑150 (MQ=255)
                                            tGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAac                                                                                                    <  2:660926/150‑1 (MQ=255)
                                             ggATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAaca                                                                                                   <  2:96439/150‑1 (MQ=255)
                                               aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACg                                                                                                 >  1:11299/1‑150 (MQ=255)
                                                 gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTAGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAt                                                                                               >  3:402932/1‑150 (MQ=255)
                                                   agaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGa                                                                                             <  1:155128/150‑1 (MQ=255)
                                                         ccACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                                                                                       >  2:548219/1‑150 (MQ=255)
                                                          cACTTCGTAGAAGCCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAc                                                                                      <  2:334386/150‑1 (MQ=255)
                                                           aCTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAcc                                                                                     <  4:354294/150‑1 (MQ=255)
                                                            cTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                                                                                         >  3:462088/1‑129 (MQ=255)
                                                            cTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTGGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCt                                                                                                         <  4:462088/129‑1 (MQ=255)
                                                                 tAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGCTCCTGGCACACGATGACGCCAACCGTGCAt                                                                               >  2:402160/1‑150 (MQ=255)
                                                                  agaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAggtggt                                                                                                                                       <  3:125110/93‑1 (MQ=255)
                                                                  agaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAggtggt                                                                                                                                       >  4:125110/1‑93 (MQ=255)
                                                                  agaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                              >  3:342430/1‑150 (MQ=255)
                                                                  agaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGAGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                              >  4:424295/1‑150 (MQ=255)
                                                                   gaagaCCTGGTAACTTGCCGTAGCAAAGGCGACTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTg                                                                             >  2:468297/1‑150 (MQ=255)
                                                                     agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGTACACGATGACGCCAACCGTGCATTGAt                                                                           <  1:619694/150‑1 (MQ=255)
                                                                          tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                      >  2:282176/1‑150 (MQ=255)
                                                                            gTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCg                                                                    >  4:197199/1‑150 (MQ=255)
                                                                             tAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                                   <  2:652944/150‑1 (MQ=255)
                                                                                 ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                               >  3:118886/1‑150 (MQ=255)
                                                                                 ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTAGCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                               <  1:526050/150‑1 (MQ=255)
                                                                                 ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACTCCAACCGTGCATTGATGGGTGCGAACAt                                                               >  3:476996/1‑150 (MQ=255)
                                                                                    ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACg                                                                                           >  1:47428/1‑119 (MQ=255)
                                                                                    ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACg                                                                                           <  2:47428/119‑1 (MQ=255)
                                                                                         gCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTc                                                       >  1:512553/1‑150 (MQ=255)
                                                                                          cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                           <  1:568514/129‑1 (MQ=255)
                                                                                          cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                           >  2:568514/1‑129 (MQ=255)
                                                                                          cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                                      >  2:211588/1‑150 (MQ=255)
                                                                                                       aGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                         >  1:402134/1‑150 (MQ=255)
                                                                                                         cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActct                                       >  1:578318/1‑150 (MQ=255)
                                                                                                         cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTAACCCCGTCCTGGGAACACGATCACGCCAACCGTGCAGTGATGGGTGCGAACATGCAACGTCACGCCGTTCCGActct                                       >  3:403141/1‑150 (MQ=255)
                                                                                                          ttGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTg                                      >  4:203343/1‑150 (MQ=255)
                                                                                                           tGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgc                                     <  3:469667/150‑1 (MQ=255)
                                                                                                              tCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                        >  1:320048/1‑128 (MQ=255)
                                                                                                              tCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                        <  2:320048/128‑1 (MQ=255)
                                                                                                              tCAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcg                                  >  4:153056/1‑150 (MQ=255)
                                                                                                               cAGCCGCGTCCGGGTTGACTGAATGGACGTGCACTCCCAGCAGGTGGTATCCGTGGGTGCGTCCCTGATCCCGTTCATGCAACACGATGACGCCAACCGCGCATTGATGGGTGCGAACCTGCAACGTCAGGCCGCTCCGCCTCTgcgcgc                                 <  4:403141/150‑1 (MQ=255)
                                                                                                               cAGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcgc                                 <  1:263564/150‑1 (MQ=255)
                                                                                                                aGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                           >  3:268671/1‑139 (MQ=255)
                                                                                                                aGCCGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                           <  4:268671/139‑1 (MQ=255)
                                                                                                                  ccGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCACCGTCAGGCCGTTCCGACTCTGCGCGCTGa                              <  2:512553/150‑1 (MQ=255)
                                                                                                                  ccGCGACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGa                              <  1:282176/150‑1 (MQ=255)
                                                                                                                   cgcgACCAGGTTGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGAt                             >  3:76997/1‑150 (MQ=255)
                                                                                                                          aGGTTGACTACATGGACGTATCCTCCCAGCAGGTAGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGc                      <  4:402932/150‑1 (MQ=255)
                                                                                                                              tGACTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                  <  4:342430/150‑1 (MQ=255)
                                                                                                                                 cTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                               <  2:346209/102‑1 (MQ=255)
                                                                                                                                 cTACATGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                               >  1:346209/1‑102 (MQ=255)
                                                                                                                                     aTGGACGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACt           <  3:203343/150‑1 (MQ=255)
                                                                                                                                      tGGACNTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTg          <  3:134979/150‑1 (MQ=255)
                                                                                                                                      tGGACGTATCCTCCCAACAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTg          <  4:169493/150‑1 (MQ=255)
                                                                                                                                          cGTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTAt      <  4:512636/150‑1 (MQ=255)
                                                                                                                                           gTATCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATg     <  4:92712/150‑1 (MQ=255)
                                                                                                                                             aTCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa   >  3:261397/1‑150 (MQ=255)
                                                                                                                                             aTCCTCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa   >  1:356545/1‑150 (MQ=255)
                                                                                                                                              tcctccCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGaa  >  2:574007/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAA  >  NC_000913_3_hsa_tpiA/4183050‑4183341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑