Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 3,535,612 (GA)4→5 coding (257/1353 nt) envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA3,535,6031.A88.8% 551.3 / 63.0 169coding (266/1353 nt)envZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base . (9/10);  new base A (72/78);  total (81/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913_3_hsa_tpiA3,535,6032.G88.7% 526.9 / 68.2 169coding (266/1353 nt)envZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base . (9/10);  new base G (72/77);  total (81/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

GCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑AGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGG  >  NC_000913_3_hsa_tpiA/3535454‑3535743
                                                                                                                                                      ||                                                                                                                                                
 ccAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑a                                                                                                                                                 >  1:336570/1‑150 (MQ=255)
 ccAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑a                                                                                                                                                 >  2:308715/1‑150 (MQ=255)
  cAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑ag                                                                                                                                                <  3:243160/150‑1 (MQ=255)
   aGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑aaa                                                                                                                                               >  1:1306977/1‑148 (MQ=255)
     gTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑aga‑‑ga                                                                                                                                           <  4:678235/150‑1 (MQ=255)
           aGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagaga                                                                                                                                     <  2:1351433/150‑3 (MQ=255)
             ccAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCGCTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatc                                                                                                                                   <  4:547520/150‑5 (MQ=255)
             ccAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatc                                                                                                                                   >  2:10946/1‑146 (MQ=255)
             ccAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatc                                                                                                                                   <  2:336570/150‑5 (MQ=255)
             ccAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatc                                                                                                                                   <  4:476048/150‑5 (MQ=255)
             ccAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatc                                                                                                                                   >  4:974770/1‑146 (MQ=255)
               aGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatccc                                                                                                                                 <  2:1630651/150‑7 (MQ=255)
               aGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatccc                                                                                                                                 >  4:831371/1‑144 (MQ=255)
               aGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑Aga‑‑gagagagatccc                                                                                                                                 >  3:399231/1‑144 (MQ=255)
                      aGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑AGAACGAGCGA‑‑TCCCCCGCTCa                                                                                                                          >  2:1432199/1‑150 (MQ=255)
                       ggCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCAc                                                                                                                         >  2:1403550/1‑150 (MQ=255)
                        gCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACg                                                                                                                        >  3:108196/1‑150 (MQ=255)
                        gCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACg                                                                                                                        >  3:74394/1‑150 (MQ=255)
                         cGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACgg                                                                                                                       <  1:409107/150‑1 (MQ=255)
                         cGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACgg                                                                                                                       <  4:74394/150‑1 (MQ=255)
                           aaCTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTa                                                                                                                     <  3:796481/150‑1 (MQ=255)
                              ttttGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGAt                                                                                                                  >  3:753623/1‑150 (MQ=255)
                              ttttGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGAt                                                                                                                  >  3:753825/1‑150 (MQ=255)
                              ttttGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGAt                                                                                                                  >  2:198905/1‑150 (MQ=255)
                                  gttgACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTcc                                                                                                              <  2:59080/150‑1 (MQ=255)
                                       ccTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACg                                                                                                         >  1:1032563/1‑150 (MQ=255)
                                       ccTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACg                                                                                                         <  1:1411901/150‑1 (MQ=255)
                                        cTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACgg                                                                                                        >  1:626547/1‑150 (MQ=255)
                                        cTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACgg                                                                                                        >  3:984893/1‑150 (MQ=255)
                                          cAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGaa                                                                                                      >  3:1018504/1‑150 (MQ=255)
                                             cgcgCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGc                                                                                                   <  3:465337/150‑1 (MQ=255)
                                             cgcgCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑aga‑‑gaga                                                                                                                                         <  3:906068/112‑1 (MQ=255)
                                              gcgcTCTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCg                                                                                                  <  4:993604/150‑1 (MQ=255)
                                              gcgcACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCg                                                                                                  <  2:535187/150‑1 (MQ=255)
                                                 cACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGa                                                                                               <  1:198905/150‑1 (MQ=255)
                                                 cACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGa                                                                                               <  1:270623/150‑1 (MQ=255)
                                                    ttCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                                                            >  3:544652/1‑150 (MQ=255)
                                                    ttCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                                                            >  2:937726/1‑150 (MQ=255)
                                                     tCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGca                                                                                           >  4:191112/1‑150 (MQ=255)
                                                      ccGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                                                          >  2:1286716/1‑150 (MQ=255)
                                                         tCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCAGGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaac                                                                                       >  1:1331172/1‑150 (MQ=255)
                                                            ggCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaaccaa                                                                                    >  1:1504091/1‑150 (MQ=255)
                                                             gccgccCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑AGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTg                                                                                 <  3:231845/150‑1 (MQ=255)
                                                                     gTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGcc                                                                           >  4:877550/1‑150 (MQ=255)
                                                                        gctgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                                                        <  3:998419/150‑1 (MQ=255)
                                                                         ctgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcc                                                                       >  1:596516/1‑150 (MQ=255)
                                                                            cgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCGGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctcc                                                                    <  2:1512461/150‑1 (MQ=255)
                                                                             gcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCa                                                                   <  1:643960/150‑1 (MQ=255)
                                                                              ccATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAg                                                                  <  1:10946/150‑1 (MQ=255)
                                                                               cATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                                                                 >  1:1057471/1‑150 (MQ=255)
                                                                               cATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                                                                 <  4:108196/150‑1 (MQ=255)
                                                                                  cTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGc                                                              <  3:191112/150‑1 (MQ=255)
                                                                                   tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGAc                                                                                                          >  1:881113/1‑105 (MQ=255)
                                                                                   tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc                                                                            >  1:579729/1‑135 (MQ=255)
                                                                                   tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCa                                                             >  1:804119/1‑150 (MQ=255)
                                                                                      tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGtt                                                          >  1:695464/1‑150 (MQ=255)
                                                                                       ggCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGa                                                                                               >  2:1323964/1‑112 (MQ=255)
                                                                                         cTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGt                                                       <  3:309636/150‑1 (MQ=255)
                                                                                          ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAgg                                                                                             <  4:585487/111‑1 (MQ=255)
                                                                                          ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAgg                                                                                             >  3:585487/1‑111 (MQ=255)
                                                                                          ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTc                                                      >  2:829171/1‑150 (MQ=255)
                                                                                          ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAAATGCGTGCCGTCCTCCAGTTGCAGTTTGTc                                                      <  1:943647/150‑1 (MQ=255)
                                                                                           tAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCg                                                     <  3:839749/150‑1 (MQ=255)
                                                                                            aaGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCgg                                                    >  1:455198/1‑150 (MQ=255)
                                                                                              gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc                                                  <  1:1383352/150‑1 (MQ=255)
                                                                                              gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc                                                  >  2:571121/1‑150 (MQ=255)
                                                                                              gAATGCATAGTGTTGCGCCCAACGCAGGCGTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑AGA‑‑GAGAGAGGTCCCCAGCTCACCGTAGATCTCCCGACGGAAGGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc                                                  <  2:1150329/150‑1 (MQ=255)
                                                                                                 ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc                                               <  3:877550/150‑1 (MQ=255)
                                                                                                  tCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatca                                              <  2:1504091/150‑1 (MQ=255)
                                                                                                   cATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑AGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa                                           >  3:933001/1‑150 (MQ=255)
                                                                                                    aTAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAAc                                            <  4:399231/150‑1 (MQ=255)
                                                                                                    aTAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAAc                                            <  3:652252/150‑1 (MQ=255)
                                                                                                    aTAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAAc                                            <  1:671368/150‑1 (MQ=255)
                                                                                                    aTAGTGTTGCGCCCAACGCAGACCTGCCTCTGCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAAc                                            <  2:62292/150‑1 (MQ=255)
                                                                                                      aGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt                                          >  4:584758/1‑150 (MQ=255)
                                                                                                      aGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt                                          >  4:1064487/1‑150 (MQ=255)
                                                                                                       gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa                                         <  1:1443443/150‑1 (MQ=255)
                                                                                                         gtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACg                                       <  2:1489196/150‑1 (MQ=255)
                                                                                                          ttGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCt                                                                                                                            <  4:85165/64‑1 (MQ=255)
                                                                                                             cgcCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCAAGTTGCAGTTTGTCGGTCATCAACATACGCACt                                   >  4:423810/1‑150 (MQ=255)
                                                                                                               cccAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGa                                                                                               >  2:993043/1‑88 (MQ=255)
                                                                                                               cccAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTc                                 >  1:919093/1‑150 (MQ=255)
                                                                                                                ccAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCg                                >  1:198096/1‑150 (MQ=255)
                                                                                                                ccAACGCAGACCTGCCTCTTCGGCAGCATCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCg                                >  1:1310436/1‑150 (MQ=255)
                                                                                                                 cAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGt                               <  4:412380/150‑1 (MQ=255)
                                                                                                                 cAACGCAGACCTGCATCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAATGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGt                               <  2:979266/150‑1 (MQ=255)
                                                                                                                     gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTAcgc                           >  1:523860/1‑150 (MQ=255)
                                                                                                                       aGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGa                         <  1:512912/150‑1 (MQ=255)
                                                                                                                           cTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGAc                     >  2:50960/1‑150 (MQ=255)
                                                                                                                            tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCa                                                             <  2:402633/109‑1 (MQ=255)
                                                                                                                                 ctTCGGCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTttatt               <  1:1767284/150‑1 (MQ=255)
                                                                                                                                      gCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGATGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAAct          >  4:118836/1‑150 (MQ=255)
                                                                                                                                      gCAGCCTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCg                                >  4:770868/1‑128 (MQ=255)
                                                                                                                                          ccTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGtt                                                                >  1:986302/1‑92 (MQ=255)
                                                                                                                                           cTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTcc                                                                                                              <  3:417630/45‑1 (MQ=255)
                                                                                                                                           cTCGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAActgctg      <  4:103133/149‑1 (MQ=255)
                                                                                                                                           cTCGTTGGAGT‑‑AGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAg   <  1:1239456/150‑1 (MQ=255)
                                                                                                                                             cGTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAg   <  1:231670/150‑1 (MQ=255)
                                                                                                                                              gTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCg                                                     <  1:541975/99‑1 (MQ=255)
                                                                                                                                              gTTGGAGTAGAGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTCTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTCAACTGCTGGAgg  >  2:1404924/1‑150 (MQ=255)
                                                                                                                                                      ||                                                                                                                                                
GCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGT‑‑AGA‑‑GAGAGA‑‑TCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGG  >  NC_000913_3_hsa_tpiA/3535454‑3535743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑