Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,159,133 C→A A221E (GCG→GAG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,159,1330CA88.6% 569.4 / 63.5 202A221E (GCG→GAG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (13/10);  new base A (93/85);  total (107/95)
Fisher's exact test for biased strand distribution p-value = 8.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01

Only 100 of 211 total aligned reads displayed.

ATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCC  >  NC_000913_3_hsa_tpiA/4158986‑4159279
                                                                                                                                                   |                                                                                                                                                  
   aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCa                                                                                                                                               >  1:1909180/1‑150 (MQ=255)
     ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGc                                                                                                                                             <  2:2373544/150‑1 (MQ=255)
     ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGc                                                                                                                                             <  2:1676044/150‑1 (MQ=255)
      cGTGGGCGAACCGCGAATGCGTACCGAGGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGcc                                                                                                                                            <  2:1450947/150‑1 (MQ=255)
       gTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCt                                                                                                                                           >  1:1324402/1‑150 (MQ=255)
          ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGa                                                                                                                                        <  3:554916/150‑1 (MQ=255)
           gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGaa                                                                                                                                       >  3:835608/1‑150 (MQ=255)
           gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGaa                                                                                                                                       >  3:534525/1‑150 (MQ=255)
            cGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGaaa                                                                                                                                      <  1:2035344/150‑1 (MQ=255)
              aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct                                                                                                                                    >  4:676057/1‑150 (MQ=255)
                 cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTg                                                                                                                                 >  2:1237331/1‑150 (MQ=255)
                  gcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTgc                                                                                                                                >  4:358117/1‑150 (MQ=255)
                  gcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTgc                                                                                                                                <  4:176835/150‑1 (MQ=255)
                   cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTgcg                                                                                                                               >  1:2299634/1‑150 (MQ=255)
                          gTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCaccctg                                                                                                                        >  3:1206811/1‑145 (MQ=255)
                           tACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACAtgg                                                                                                                       <  1:982157/150‑1 (MQ=255)
                            aCCTATGGCCGATCTGGCAGGGGAAAAACTGCTGATGTTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTCTCTGTTTTGAAGCCGGGGCGGACGAAGATACACACTTCCGCGAGACCAGCCTGGAAACACTGCGCAACAtggt                                                                                                                      >  2:1785655/1‑150 (MQ=255)
                               gATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTggc                                                                                                                   >  2:680581/1‑150 (MQ=255)
                                aTGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTggcg                                                                                                                  >  1:397501/1‑150 (MQ=255)
                                   gCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCgaga                                                                                                                                                  <  1:1388370/115‑1 (MQ=255)
                                   gCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCgaga                                                                                                                                                  >  2:1388370/1‑115 (MQ=255)
                                    ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                                                                                              >  2:228415/1‑150 (MQ=255)
                                    ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                                                                                              <  3:1050898/150‑1 (MQ=255)
                                    ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                                                                                              <  2:220828/150‑1 (MQ=255)
                                     cGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAgg                                                                                                             <  4:955742/150‑1 (MQ=255)
                                     cGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                                                                                              >  2:1224699/1‑149 (MQ=255)
                                     cGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGGTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAgg                                                                                                             >  3:962032/1‑150 (MQ=255)
                                      gATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGt                                                                                                            >  1:1806488/1‑150 (MQ=255)
                                       aTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCCGCGCAACATGGTGGCGGCCGGTa                                                                                                           >  1:56976/1‑150 (MQ=255)
                                        tCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAg                                                                                                          >  2:337516/1‑150 (MQ=255)
                                         cTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGc                                                                                                         <  3:693789/150‑1 (MQ=255)
                                            gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACAtg                                                                                                                        <  3:1526034/132‑1 (MQ=255)
                                            gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg                                                                                                      >  1:1032597/1‑150 (MQ=255)
                                              gggggAAAAACTGCGGGTGCTGGAAGATGGTCACGGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAACCTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                                                                                    <  4:1447593/149‑1 (MQ=255)
                                              aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                                                                                    >  1:1278420/1‑150 (MQ=255)
                                                  gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGACCACt                                                                                                >  2:2155758/1‑150 (MQ=255)
                                                   aaaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGCTCACtt                                                                                               >  4:362387/1‑150 (MQ=255)
                                                    aaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACttt                                                                                              <  1:1188097/150‑1 (MQ=255)
                                                     aaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGTGATGTGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa                                                                                             <  1:24339/150‑1 (MQ=255)
                                                     aaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa                                                                                             >  3:799123/1‑150 (MQ=255)
                                                            tgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAg                                                                                      <  4:534525/150‑1 (MQ=255)
                                                             gATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgc                                                                                     >  1:1019919/1‑150 (MQ=255)
                                                              aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGa                                                                                                                                        >  2:2004716/1‑98 (MQ=255)
                                                              aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgcg                                                                                    >  1:1096883/1‑150 (MQ=255)
                                                                  tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg                                                                                >  2:2341150/1‑150 (MQ=255)
                                                                    gAAGATGGTCACTGTTTGCGCGATCAGGCACTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggct                                                                              >  2:1586650/1‑150 (MQ=255)
                                                                     aaGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggctg                                                                             <  4:734684/150‑1 (MQ=255)
                                                                       gATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgcgc                                                                                   >  3:342656/1‑142 (MQ=255)
                                                                            tCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCCGGTCGCGGGATCACTTTACTGCCAGCGCTGGCTGTggcgcc                                                                      >  4:944108/1‑150 (MQ=255)
                                                                             cACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGccgccg                                                                     >  2:649601/1‑150 (MQ=255)
                                                                             cACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACAGCCAGCgctggcagtaaa                                                                         >  2:1842751/1‑140 (MQ=255)
                                                                                tGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAg                                                                  >  1:768730/1‑150 (MQ=255)
                                                                                tGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAg                                                                  <  2:1417874/150‑1 (MQ=255)
                                                                                 gTTTGCGTGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                 <  3:1441499/150‑1 (MQ=255)
                                                                                 gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGTAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCGGCGCTGGCTGTGCCGCCGGAgc                                                                 <  4:485041/150‑1 (MQ=255)
                                                                                 gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                 <  1:121316/150‑1 (MQ=255)
                                                                                 gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATAAACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAGCTCTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                 <  1:1247951/150‑1 (MQ=255)
                                                                                  tttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                                                                <  3:1591745/150‑1 (MQ=255)
                                                                                   ttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcgc                                                               <  4:799123/150‑1 (MQ=255)
                                                                                     gcgcGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaa                                                             >  2:813255/1‑150 (MQ=255)
                                                                                     gcgcGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaa                                                             >  3:153904/1‑150 (MQ=255)
                                                                                       gcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAc                                                           <  1:881713/150‑1 (MQ=255)
                                                                                         gATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAcgc                                                         <  2:1278420/150‑1 (MQ=255)
                                                                                          aTCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAcgcg                                                        >  1:1941036/1‑150 (MQ=255)
                                                                                           tCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCCGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTAc                                                                                            >  1:1235976/1‑113 (MQ=255)
                                                                                           tCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGa                                                       <  4:239245/150‑1 (MQ=255)
                                                                                            ggggCAATGGGTTTCTGTTGGGAAGCCGGGGCGGATGAGGATACACACTGCCGGGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCGGGTAGCGGGGTCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt                                                      <  2:1307868/148‑1 (MQ=255)
                                                                                             aGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                                                                <  3:208652/139‑1 (MQ=255)
                                                                                             aGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATg                                                     <  4:962187/150‑1 (MQ=255)
                                                                                              ggCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATgg                                                    >  1:1589191/1‑150 (MQ=255)
                                                                                              ggCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgcc                                                                         <  2:288709/129‑1 (MQ=255)
                                                                                              ggCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgcc                                                                         >  1:288709/1‑129 (MQ=255)
                                                                                                   tGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCAGTGCCGCCGGAGCGCAAACGCGATGGGgttg                                               >  4:312452/1‑150 (MQ=255)
                                                                                                       tttCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTa                                           >  2:1191998/1‑150 (MQ=255)
                                                                                                       tttCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTa                                           >  2:1092299/1‑150 (MQ=255)
                                                                                                          cTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTa                                           <  4:1336274/147‑1 (MQ=255)
                                                                                                          cTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTa                                           >  3:1336274/1‑147 (MQ=255)
                                                                                                          cTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCt                                        <  3:471356/150‑1 (MQ=255)
                                                                                                           tGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTg                                       <  4:841554/150‑1 (MQ=255)
                                                                                                               ttGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGt                                   <  1:1703916/150‑1 (MQ=255)
                                                                                                                tGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                                                                                   >  1:2499014/1‑85 (MQ=255)
                                                                                                                tGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                                                                                   <  2:2499014/85‑1 (MQ=255)
                                                                                                                 gAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc                                 <  1:337516/150‑1 (MQ=255)
                                                                                                                    gCCGGGGCGGATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAtt                              <  1:2234018/150‑1 (MQ=255)
                                                                                                                            ggATGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGa                      >  4:1547749/1‑150 (MQ=255)
                                                                                                                             gATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGaa                     <  4:1110777/150‑1 (MQ=255)
                                                                                                                              aTGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGcc                         >  1:1646571/1‑145 (MQ=255)
                                                                                                                              aTGAAGATACACACTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAAc                    >  1:656097/1‑150 (MQ=255)
                                                                                                                                      acacacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCtgt                                                                            >  1:1226145/1‑86 (MQ=255)
                                                                                                                                      acacacTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAc                                                                                                 <  2:1028955/65‑1 (MQ=255)
                                                                                                                                       cacacTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGTCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCa           <  3:1226989/150‑1 (MQ=255)
                                                                                                                                        acacTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATgggg                                                  <  1:937285/110‑1 (MQ=255)
                                                                                                                                        acacTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATgggg                                                  >  2:937285/1‑110 (MQ=255)
                                                                                                                                        acacTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGAGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCAc          >  2:1140412/1‑150 (MQ=255)
                                                                                                                                        acacTTCCGCGAGACCAGCCGGGAAACTCTGCGCAACATGGTGGCGGCAGGCAGCGGGATCACTTTACTGCCAGCGCTGGCTCTGCTGCCGGAGCGCAAACGCGATGGGGCTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCAc          >  4:21491/1‑150 (MQ=255)
                                                                                                                                         cacTTCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACt         <  1:2356870/150‑1 (MQ=255)
                                                                                                                                            ttCCGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTAtt      >  2:193250/1‑150 (MQ=255)
                                                                                                                                              ccGCGAGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTgg    <  2:2397641/150‑1 (MQ=255)
                                                                                                                                               cgcgagACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGc   <  2:397501/150‑1 (MQ=255)
                                                                                                                                               cgcgagACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGc   <  3:995123/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCC  >  NC_000913_3_hsa_tpiA/4158986‑4159279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: