Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 1,158,559 2 bp→TC coding (691‑692/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,158,5590CT95.7% 376.5 / 4.1 117Q231S (CAG→TCG) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (62/50);  total (65/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01
*NC_000913_3_hsa_tpiA1,158,5600AC95.7% 393.8 / 3.9 117Q231S (CAG→TCG) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (60/50);  total (65/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 118 total aligned reads displayed.

TCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGG  >  NC_000913_3_hsa_tpiA/1158415‑1158709
                                                                                                                                                ||                                                                                                                                                     
tctcAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGtt                                                                                                                                                   <  4:805529/150‑1 (MQ=255)
 ctcAGTGGGCTGCTTACCAGAGCCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGttt                                                                                                                                                  <  2:1352852/150‑1 (MQ=255)
    aGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTcc                                                                                                                                               <  4:190289/150‑1 (MQ=255)
    aGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTcc                                                                                                                                               <  1:810543/150‑1 (MQ=255)
      tGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCAc                                                                                                                                             >  2:1204843/1‑150 (MQ=255)
        ggCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGGGAATACACCAACGCAGCAGGTTCGGTTTTCCACgg                                                                                                                                           <  4:337493/150‑1 (MQ=255)
             ctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACa                                                                                                                                      <  3:12984/150‑1 (MQ=255)
                 ccAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTcc                                                                                                                                  >  2:1034835/1‑150 (MQ=255)
                 ccAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTcc                                                                                                                                  >  4:435616/1‑150 (MQ=255)
                     aaCCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGt                                                                                                                              >  3:920065/1‑150 (MQ=255)
                        ccGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTtat                                                                                                                           <  4:529862/150‑1 (MQ=255)
                               ttGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCgg                                                                                                                    >  1:1348661/1‑150 (MQ=255)
                                   gTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGa                                                                                                                >  1:680428/1‑150 (MQ=255)
                                    tttGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGt                                                                                                                              >  1:63478/1‑135 (MQ=255)
                                    tttGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGt                                                                                                                              <  2:63478/135‑1 (MQ=255)
                                    tttGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGAc                                                                                                               >  1:235033/1‑150 (MQ=255)
                                      tGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGAccc                                                                                                             <  4:106909/150‑1 (MQ=255)
                                        gCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCcg                                                                                                                                 <  4:655921/128‑1 (MQ=255)
                                           tttACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGtt                                                                                                                             <  1:588026/129‑1 (MQ=255)
                                           tttACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGtt                                                                                                                             >  2:588026/1‑129 (MQ=255)
                                           tttACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTg                                                                                                        >  1:432110/1‑150 (MQ=255)
                                              aCGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                                     <  3:357781/150‑1 (MQ=255)
                                                ggTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGt                                                                                                   >  1:1375680/1‑150 (MQ=255)
                                                  tttCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTaa                                                                                                 >  2:208184/1‑150 (MQ=255)
                                                  tttCATCGAACGTTGCCTGGTACCGGTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGATCGGTTTTCCACGGCGACATTCCGCGTTCGATGGCGGGTGACCCTACTGCGGGGaa                                                                                                 >  4:400783/1‑150 (MQ=255)
                                                    tCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAAc                                                                                               <  2:68004/150‑1 (MQ=255)
                                                    tCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAAc                                                                                               >  2:425838/1‑150 (MQ=255)
                                                       tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGt                                                                                            >  3:679286/1‑150 (MQ=255)
                                                       tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGt                                                                                            >  1:152765/1‑150 (MQ=255)
                                                       tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAAAGCAATAGATTGGGATATGCACAGAAGCATACAGTGTTATATAGTGGGGGACCAGACTGAGGGTAAACTGt                                                                                            >  3:796838/1‑150 (MQ=255)
                                                        cGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTc                                                                                           >  1:931936/1‑150 (MQ=255)
                                                         gAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGCCCGTCCCTTTCCCGATGCAAATTGGTTAATGTACCAGCGTAGGAGGTTGGGCTTTGCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCt                                                                                          >  1:298521/1‑150 (MQ=255)
                                                              ttGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTaaa                                                                                                >  1:1445613/1‑139 (MQ=255)
                                                              ttGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTaaa                                                                                                <  2:1445613/139‑1 (MQ=255)
                                                                 ccTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCt                                                                                          >  4:541530/1‑142 (MQ=255)
                                                                 ccTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCt                                                                                          <  3:541530/142‑1 (MQ=255)
                                                                  cTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                 <  3:113777/150‑1 (MQ=255)
                                                                  cTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                 <  1:731676/150‑1 (MQ=255)
                                                                   tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTcc                                                                                >  1:102224/1‑150 (MQ=255)
                                                                      tACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGt                                                                             <  1:1204843/150‑1 (MQ=255)
                                                                       aCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGTGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGtt                                                                            <  2:869965/150‑1 (MQ=255)
                                                                           tttGGTCTGCACCACATCTGGAACGTACCTTTCCAGCTGCAGATTGGTGAATACCCCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTAATATGGAGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaaa                                                                        >  4:163209/1‑150 (MQ=255)
                                                                            ttGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaaaa                                                                       <  2:660554/150‑1 (MQ=255)
                                                                                tCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTa                                                                   <  4:83314/150‑1 (MQ=255)
                                                                                  tGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACg                                                                 >  2:828700/1‑150 (MQ=255)
                                                                                  tGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACg                                                                 >  2:610388/1‑150 (MQ=255)
                                                                                   gCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACgg                                                                >  2:1037190/1‑150 (MQ=255)
                                                                                     accacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTc                                                              <  2:931936/150‑1 (MQ=255)
                                                                                      ccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGAc                                                                                                          <  2:1050345/105‑1 (MQ=255)
                                                                                      ccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGAc                                                                                                          >  1:1050345/1‑105 (MQ=255)
                                                                                       cacaTCTGGAACGTACCTTTACAGATGCAAATTGGTGAATACACCAACGCGGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCTACTGCGGGTAAACTGTCTGGGGGCTTCCTGTTCAAAATGTACGGACTg                                                            >  4:763410/1‑150 (MQ=255)
                                                                                        gcaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGtttt                                                                                                                                                 >  3:854776/2‑64 (MQ=255)
                                                                                        gcaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGtttt                                                                                                                                                 <  4:854776/63‑1 (MQ=255)
                                                                                          aTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCa                                                         >  1:54151/1‑150 (MQ=255)
                                                                                           tCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAg                                                        <  2:1375680/150‑1 (MQ=255)
                                                                                            cTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGc                                                       <  2:680428/150‑1 (MQ=255)
                                                                                             tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCt                                                      >  3:739736/1‑150 (MQ=255)
                                                                                             tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCt                                                      >  1:414848/1‑150 (MQ=255)
                                                                                              ggAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTg                                                     >  3:599701/1‑150 (MQ=255)
                                                                                                aaCGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGcc                                                   <  1:828700/150‑1 (MQ=255)
                                                                                                    tACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                               >  2:270067/1‑150 (MQ=255)
                                                                                                    tACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                               >  3:522219/1‑150 (MQ=255)
                                                                                                     aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAt                                              <  1:73414/150‑1 (MQ=255)
                                                                                                      ccTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAtt                                             >  2:548266/1‑150 (MQ=255)
                                                                                                       cTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                            >  2:705189/1‑150 (MQ=255)
                                                                                                       cTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                            <  2:270390/150‑1 (MQ=255)
                                                                                                        tttCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGc                                                           <  4:715353/134‑1 (MQ=255)
                                                                                                        tttCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGc                                                           >  3:715353/1‑134 (MQ=255)
                                                                                                         ttCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTc                                                                           <  4:738077/117‑1 (MQ=255)
                                                                                                         ttCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTc                                                                           >  3:738077/1‑117 (MQ=255)
                                                                                                          tCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTa                                         >  2:1345149/1‑150 (MQ=255)
                                                                                                              gATGCAGATTGGTGAATACCCCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTg                                     <  2:1482176/150‑1 (MQ=255)
                                                                                                                 gCAGATTGGTGAGTACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                                  <  4:761196/150‑1 (MQ=255)
                                                                                                                 gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                 >  2:972027/1‑103 (MQ=255)
                                                                                                                 gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                                 <  1:972027/103‑1 (MQ=255)
                                                                                                                 gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGc                                                       <  1:930040/129‑1 (MQ=255)
                                                                                                                 gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTGTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGGGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                                  >  4:465751/1‑150 (MQ=255)
                                                                                                                   aGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggt                                                                                       >  1:1003146/1‑95 (MQ=255)
                                                                                                                   aGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggt                                                                                       >  1:1003150/1‑95 (MQ=255)
                                                                                                                   aGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggt                                                                                       <  2:1003150/95‑1 (MQ=255)
                                                                                                                   aGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggt                                                                                       <  2:1003146/95‑1 (MQ=255)
                                                                                                                    gATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCActc                               >  3:295369/1‑150 (MQ=255)
                                                                                                                        ggTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCt                           >  3:167335/1‑150 (MQ=255)
                                                                                                                         gTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTa                          <  4:295369/150‑1 (MQ=255)
                                                                                                                              tACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCa                     >  2:635461/1‑150 (MQ=255)
                                                                                                                              tACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCa                     >  1:588379/1‑150 (MQ=255)
                                                                                                                               acacCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCGAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAg                    <  2:380673/150‑1 (MQ=255)
                                                                                                                               acacCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAg                    <  2:591894/150‑1 (MQ=255)
                                                                                                                               acacCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAg                    <  1:1034835/150‑1 (MQ=255)
                                                                                                                                   cAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCt                                                                               >  1:1316272/1‑87 (MQ=255)
                                                                                                                                   cAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCt                                                                               <  2:1316272/87‑1 (MQ=255)
                                                                                                                                   cAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaaaa                <  1:1315335/150‑1 (MQ=255)
                                                                                                                                    aaCGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTg                                                     <  4:22205/112‑1 (MQ=255)
                                                                                                                                      cGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcg             >  2:292881/1‑150 (MQ=255)
                                                                                                                                      cGCAGCAGGGTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcg             >  2:292888/1‑150 (MQ=255)
                                                                                                                                       gcagcaGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAc                       >  4:621699/1‑139 (MQ=255)
                                                                                                                                       gcagcaGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAc                       <  3:621699/139‑1 (MQ=255)
                                                                                                                                         agcagGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcgc          <  1:1345149/150‑1 (MQ=255)
                                                                                                                                         agcagGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcgc          >  2:797241/1‑150 (MQ=255)
                                                                                                                                                 aggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTgg  >  2:1450519/1‑150 (MQ=255)
                                                                                                                                                ||                                                                                                                                                     
TCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGG  >  NC_000913_3_hsa_tpiA/1158415‑1158709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑